PMID- 29626590
OWN - NLM
STAT- MEDLINE
DCOM- 20180725
LR  - 20181202
IS  - 1872-8227 (Electronic)
IS  - 0168-8227 (Linking)
VI  - 140
DP  - 2018 Jun
TI  - Altered expression of WFS1 and NOTCH2 genes associated with diabetic nephropathy 
      in T2DM patients.
PG  - 304-313
LID - S0168-8227(18)30064-0 [pii]
LID - 10.1016/j.diabres.2018.03.053 [doi]
AB  - AIM: The increased incidence of type 2 diabetes mellitus (T2DM) and the 
      importance of early identification and management of its complications, 
      especially diabetic nephropathy (DN), have spotted the light on genetic factors 
      that increase risk of T2DM and its related nephropathy. The present study aimed 
      at investigating expression of (KCNJ11, ABCC8, JAZF1, WFS1, PPARG, NOTCH2 and 
      EXOSC4) genes in peripheral blood of T2DM patients. METHOD: The study included 30 
      non-complicated T2DM patients, 30 patients with DN and 40 healthy controls. 
      Quantitative Real Time PCR Array was used to study gene expression. RESULTS: 
      NOTCH2 showed higher expression while KCNJ11, JAZF1, WFS1 and PPARG genes showed 
      lower expression in DN patients compared to non-complicated patients. KCNJ11, 
      JAZF1, WFS1, PPARG, and EXOSC4 expression showed significant negative correlation 
      with microalbumin, while NOTCH2 expression was significantly positively 
      correlated with microalbumin. AS regard HbA1c and studied genes expression, there 
      was significant negative correlation between WFS1 expression and HbA1c, while 
      NOTCH2, KCNJ11, JAZF1, PPARG, EXOSC4 expression didn't show significant 
      correlation with HbA1c. Risk ratio of studied genes expression showed that WFS1 
      and NOTCH2 had highest risk ratio (30) and highest sensitivity and specificity, 
      in relation to DN and they were the best predictors in the group of studied genes 
      at cut off value of </=0.861 for WFS1 and >/=0.678 for NOTCH2. CONCLUSION: Altered 
      expression of WFS1 and NOTCH2 genes may play a role in pathogenesis and 
      development of DN in patients with T2DM. These results may contribute in early 
      identification and management of DN.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Sharaf, Sahar A
AU  - Sharaf SA
AD  - Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo 
      University, Cairo, Egypt.
FAU - Kantoush, Nagwa A
AU  - Kantoush NA
AD  - Clinical and Chemical Pathology Department, National Research Centre, Cairo, 
      Egypt.
FAU - Ayoub, Dina F
AU  - Ayoub DF
AD  - Clinical and Chemical Pathology Department, National Research Centre, Cairo, 
      Egypt.
FAU - Ibrahim, Alshaymaa A
AU  - Ibrahim AA
AD  - Clinical and Chemical Pathology Department, National Research Centre, Cairo, 
      Egypt. Electronic address: shaymaa_t@hotmail.com.
FAU - Abdelaal, Amaal A
AU  - Abdelaal AA
AD  - Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo 
      University, Cairo, Egypt.
FAU - Aziz, Rokaya Abdel
AU  - Aziz RA
AD  - Internal Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.
FAU - ElHefnawi, Mahmoud M
AU  - ElHefnawi MM
AD  - Biomedical Informatics and Chemoinformatics Group, Informatics and Systems 
      Department, Division of Engineering Research, National Research Centre, Cairo, 
      Egypt.
FAU - Ahmed, Amira N
AU  - Ahmed AN
AD  - Clinical and Chemical Pathology Department, National Research Centre, Cairo, 
      Egypt.
LA  - eng
PT  - Journal Article
DEP - 20180404
PL  - Ireland
TA  - Diabetes Res Clin Pract
JT  - Diabetes research and clinical practice
JID - 8508335
RN  - 0 (Membrane Proteins)
RN  - 0 (NOTCH2 protein, human)
RN  - 0 (Receptor, Notch2)
RN  - 0 (wolframin protein)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Diabetes Mellitus, Type 2/*complications
MH  - Diabetic Nephropathies/*etiology/pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Membrane Proteins/*metabolism
MH  - Middle Aged
MH  - Receptor, Notch2/*metabolism
OTO - NOTNLM
OT  - Diabetic nephropathy
OT  - Gene expression
OT  - Real Time PCR Array
OT  - T2DM
EDAT- 2018/04/08 06:00
MHDA- 2018/07/26 06:00
CRDT- 2018/04/08 06:00
PHST- 2018/02/19 00:00 [received]
PHST- 2018/03/18 00:00 [revised]
PHST- 2018/03/29 00:00 [accepted]
PHST- 2018/04/08 06:00 [pubmed]
PHST- 2018/07/26 06:00 [medline]
PHST- 2018/04/08 06:00 [entrez]
AID - S0168-8227(18)30064-0 [pii]
AID - 10.1016/j.diabres.2018.03.053 [doi]
PST - ppublish
SO  - Diabetes Res Clin Pract. 2018 Jun;140:304-313. doi: 
      10.1016/j.diabres.2018.03.053. Epub 2018 Apr 4.