PMID- 29626977
OWN - NLM
STAT- MEDLINE
DCOM- 20190130
LR  - 20210308
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Linking)
VI  - 64
DP  - 2018 Apr
TI  - IFNtau mediates chemotaxis, motility, metabolism and CK18 downregulation in bovine 
      trophoblast cells in vitro via STAT1 and MAPK42/44 signaling.
PG  - 17-26
LID - S0143-4004(18)30059-6 [pii]
LID - 10.1016/j.placenta.2018.02.004 [doi]
AB  - INTRODUCTION: IFNtau is the ruminant pregnancy recognition signal. In the study we 
      investigated the autocrine influence of IFNtau on bovine F3 trophoblast cells. In 
      detail chemotaxis, motility, metabolism, cell polarisation (CK18; ezrin) and the 
      underlying classical (STAT1) and non-classical (MAPK42/44) signaling pathways 
      were examined. METHODS: Cellular signaling was analysed by densitometric Western 
      blot (STAT1, MAPK42/44, proteinkinase B) and RT-PCR (IFNAR1, -2). Cellular assays 
      were carried out for chemotaxis (agarose spot assay), cell motility (live cell 
      imaging), metabolism (MTT) and cell polarisation (CK18; ezrin). In vivo-produced 
      conceptuses of gestational days (GD) 20-39 underwent immunohistochemistry (CK18; 
      ezrin) to set the in vitro findings (cell polarisation) in proportion to the 
      in vivo situation. RESULTS: IFNtau (10-1000 ng/ml) mediated dose-dependent effects. 
      10 ng/ml IFNtau induced chemotaxis and motility, whilst 1000 ng/ml led to reduced 
      chemotaxis, motility and a 92-fold activation of MAPK44. Stimulation of cells 
      with 10-1000 ng/ml IFNtau promoted metabolism (1.4-fold), increased the gene 
      expression of IFNAR1/2 (24 h) and downregulated CK18 but not ezrin. All described 
      in vitro effects were significant. Signaling, motility and metabolism could be 
      blocked by specific inhibitors (PD98059, LY294002). CK18 and ezrin expression 
      patterns in the trophoblast of in vivo conceptuses differed depending on GD. 
      DISCUSSION: IFNtau is a major factor for bovine F3 trophoblast cells and mediates a 
      variety of cellular actions ranging from chemotaxis to polarisation. IFNtau exerts 
      its effects via classical (STAT1) and non-classical (MAPK42/44) signaling 
      pathways in a dose-dependent way. We hypothesize that (dose-dependent) IFNtau 
      regulation of the cellular effects could also be essential for bovine elongation 
      and implantation.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Loch, C
AU  - Loch C
AD  - Department of Anatomy, University of Veterinary Medicine Hannover, Germany.
FAU - Haeger, J-D
AU  - Haeger JD
AD  - Department of Anatomy, University of Veterinary Medicine Hannover, Germany. 
      Electronic address: jan-dirk.haeger@tiho-hannover.de.
FAU - Pfarrer, C
AU  - Pfarrer C
AD  - Department of Anatomy, University of Veterinary Medicine Hannover, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180221
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Interferon Type I)
RN  - 0 (Keratin-18)
RN  - 0 (Pregnancy Proteins)
RN  - 0 (Receptors, Interferon)
RN  - 0 (interferon tau)
SB  - IM
MH  - Animals
MH  - Autocrine Communication
MH  - Cattle
MH  - Cell Line
MH  - *Chemotaxis
MH  - Female
MH  - Interferon Type I/*physiology
MH  - Keratin-18/metabolism
MH  - Pregnancy
MH  - Pregnancy Proteins/*physiology
MH  - Receptors, Interferon/metabolism
MH  - *Signal Transduction
MH  - Trophoblasts/*physiology
OTO - NOTNLM
OT  - Bovine implantation
OT  - Bovine trophoblast
OT  - Cell culture model
OT  - Interferon tau
EDAT- 2018/04/09 06:00
MHDA- 2019/01/31 06:00
CRDT- 2018/04/09 06:00
PHST- 2017/12/22 00:00 [received]
PHST- 2018/02/19 00:00 [revised]
PHST- 2018/02/19 00:00 [accepted]
PHST- 2018/04/09 06:00 [entrez]
PHST- 2018/04/09 06:00 [pubmed]
PHST- 2019/01/31 06:00 [medline]
AID - S0143-4004(18)30059-6 [pii]
AID - 10.1016/j.placenta.2018.02.004 [doi]
PST - ppublish
SO  - Placenta. 2018 Apr;64:17-26. doi: 10.1016/j.placenta.2018.02.004. Epub 2018 Feb 
      21.