PMID- 29627479
OWN - NLM
STAT- MEDLINE
DCOM- 20190307
LR  - 20190307
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 250
DP  - 2018 May 2
TI  - Classical swine fever virus induces pyroptosis in the peripheral lymphoid organs 
      of infected pigs.
PG  - 37-42
LID - S0168-1702(17)30956-5 [pii]
LID - 10.1016/j.virusres.2018.04.004 [doi]
AB  - Classical swine fever virus (CSFV) causes a highly lethal disease in pigs, which 
      is characterized by immunosuppression. Leukopenia is known to be a possible 
      mechanism of immunosuppression during CSFV infection. As a new and specialized 
      form of cell death, pyroptosis is the key response of the innate immune system to 
      pathogens, and is widely involved in the occurrence and development of infectious 
      diseases. However, the relationship between CSFV and pyroptosis has not been 
      explored. In this study, we investigated the occurrence of pyroptosis in pigs 
      following CSFV infection. According to qRT-PCR assay results, the prevalence of 
      this virus in peripheral lymphoid organs (tonsils, lymph nodes, and spleen) was 
      much higher than that in other organs. Severe bleeding, necrosis, and a 
      significant reduction in lymphocytes were found in the peripheral lymphoid organs 
      of CSFV-infected pigs based on histological examination. In-depth studies showed 
      that an increased ratio of deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL)-positive cells were present in the peripheral lymphoid organs of 
      the CSFV-infected group according to immunohistochemistry. Meanwhile, the p10 
      subunit and activity of caspase-1, which is a regulator of pyroptosis, the 
      N-terminal domain of gasdermin D, which is an executor of pyroptosis, and the 
      cleavage and secretion of IL-1b, which is a product of pyroptosis were increased 
      in the peripheral lymphoid organs of the CSFV-infected group. Together, these 
      results demonstrated that pyroptosis is involved in CSFV-induced cell death in 
      vivo, which provides a new understanding of the mechanism associated with 
      lymphocyte depletion and immunosuppression in pigs infected with this virus.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Yuan, Jin
AU  - Yuan J
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou; 
      People's Republic of China.
FAU - Zhu, Mengjiao
AU  - Zhu M
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou; 
      People's Republic of China.
FAU - Deng, Shaofeng
AU  - Deng S
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou; 
      People's Republic of China.
FAU - Fan, Shuangqi
AU  - Fan S
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou; 
      People's Republic of China.
FAU - Xu, Hailuan
AU  - Xu H
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou; 
      People's Republic of China.
FAU - Liao, Jiedan
AU  - Liao J
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou; 
      People's Republic of China.
FAU - Li, Peng
AU  - Li P
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou; 
      People's Republic of China.
FAU - Zheng, Jingfang
AU  - Zheng J
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou; 
      People's Republic of China.
FAU - Zhao, Mingqiu
AU  - Zhao M
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou; 
      People's Republic of China. Electronic address: zmingqiu@scau.edu.cn.
FAU - Chen, Jinding
AU  - Chen J
AD  - College of Veterinary Medicine, South China Agricultural University, Guangzhou; 
      People's Republic of China. Electronic address: jdchen@scau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180405
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Interleukin-1beta)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Animals
MH  - Caspase 1/metabolism
MH  - Classical Swine Fever/immunology/*pathology
MH  - Classical Swine Fever Virus/*pathogenicity
MH  - Immunocompromised Host
MH  - Interleukin-1beta/immunology
MH  - Leukocytes, Mononuclear/virology
MH  - Leukopenia/immunology/*veterinary/virology
MH  - Lymph Nodes/cytology/*virology
MH  - *Pyroptosis
MH  - Swine
OTO - NOTNLM
OT  - Classical swine fever virus (CSFV)
OT  - IL-1b
OT  - In vivo
OT  - Peripheral lymphoid organ
OT  - Pyroptosis
EDAT- 2018/04/09 06:00
MHDA- 2019/03/08 06:00
CRDT- 2018/04/09 06:00
PHST- 2018/01/12 00:00 [received]
PHST- 2018/03/29 00:00 [revised]
PHST- 2018/04/03 00:00 [accepted]
PHST- 2018/04/09 06:00 [pubmed]
PHST- 2019/03/08 06:00 [medline]
PHST- 2018/04/09 06:00 [entrez]
AID - S0168-1702(17)30956-5 [pii]
AID - 10.1016/j.virusres.2018.04.004 [doi]
PST - ppublish
SO  - Virus Res. 2018 May 2;250:37-42. doi: 10.1016/j.virusres.2018.04.004. Epub 2018 
      Apr 5.