PMID- 29631685
OWN - NLM
STAT- MEDLINE
DCOM- 20180911
LR  - 20191210
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 64
IP  - 4
DP  - 2018 Mar 31
TI  - DNA repair and apoptosis: Roles in radiotherapy-related acute reactions in breast 
      cancer patients.
PG  - 64-70
AB  - Normal tissue reactions are therapy limiting factor for the effectiveness of the 
      radiotherapy in cancer patients. DNA repair and apoptosis are estimated to be 
      critical players of adverse effects in response to radiotherapy. Our aim was to 
      define the association of DNA repair (ERCC1 and XPC) and apoptotic (BCL2, CASP3 
      and NFKB1) gene expression, DNA damage levels, apoptosis changes and DNA repair 
      gene variations with the risk of acute side effects in breast cancer patients. 
      The study included 100 women with newly diagnosed breast cancer; an experimental 
      case group (n=50) with acute side effects and the control group (n=50) without 
      side effects. Gene expression was analyzed by reverse transcriptase-quantitative 
      polymerase chain reaction (RT-qPCR). Micronucleus (MN) and 
      8-hydroxy-2'-deoxyguanosine (8-OHdG) assays were performed to compare the DNA 
      damage levels. Apoptosis was examined by TDT-mediated dUTP-biotin nick 
      end-labeling (TUNEL) staining. ERCC1 rs3212986 and XPC rs3731055 polymorphisms 
      were genotyped by real-time PCR technique. No significantly correlation of DNA 
      repair and apoptosis gene expression and DNA damage levels with acute side 
      effects in response to radiotherapy. Also, there was no association between 
      apoptosis levels and acute effects. ERCC1 rs3212986 CC genotype showed a 
      protective effect against radiotherapy-induced acute reactions (p<0.001; OR: 
      0.21; 95% CI= 0.08-0.52). Our results suggest that apoptosis and DNA damage 
      levels are not associated with acute radiosensitivity. DNA repair may affect the 
      risk of acute reactions. Further studies are needed to validate the current 
      findings.
FAU - Batar, Bahadir
AU  - Batar B
AD  - Department of Medical Biology, Namik Kemal University Medical Faculty, Tekirdag, 
      Turkey.
FAU - Mutlu, Tuba
AU  - Mutlu T
AD  - Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, 
      Istanbul, Turkey.
FAU - Bostanci, Merve
AU  - Bostanci M
AD  - Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, 
      Istanbul, Turkey.
FAU - Akin, Mustafa
AU  - Akin M
AD  - Department of Clinical Radiation Oncology, Cerrahpasa Medical Faculty, Istanbul 
      University, Istanbul, Turkey.
FAU - Tuncdemir, Matem
AU  - Tuncdemir M
AD  - Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, 
      Istanbul, Turkey.
FAU - Bese, Nuran
AU  - Bese N
AD  - Department of Clinical Radiation Oncology, Cerrahpasa Medical Faculty, Istanbul 
      University, Istanbul, Turkey.
FAU - Guven, Mehmet
AU  - Guven M
AD  - Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, 
      Istanbul, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20180331
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (BCL2 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NF-kappa B p50 Subunit)
RN  - 0 (NFKB1 protein, human)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 156533-34-5 (XPC protein, human)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - EC 3.1.- (ERCC1 protein, human)
RN  - EC 3.1.- (Endonucleases)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - G9481N71RO (Deoxyguanosine)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Adult
MH  - Apoptosis/radiation effects
MH  - Breast Neoplasms/genetics/metabolism/pathology/*radiotherapy
MH  - Carcinoma, Ductal/genetics/metabolism/pathology/*radiotherapy
MH  - Carcinoma, Lobular/genetics/metabolism/pathology/*radiotherapy
MH  - Caspase 3/genetics/metabolism
MH  - DNA Fragmentation/radiation effects
MH  - DNA Repair/radiation effects
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Deoxyguanosine/analogs & derivatives/metabolism
MH  - Endonucleases/*genetics/metabolism
MH  - Female
MH  - Gamma Rays/*adverse effects
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Micronucleus Tests
MH  - Middle Aged
MH  - NF-kappa B p50 Subunit/genetics/metabolism
MH  - Polymorphism, Single Nucleotide
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
MH  - Radiation Tolerance
MH  - Signal Transduction
MH  - Skin/metabolism/pathology/*radiation effects
OTO - NOTNLM
OT  - Acute side effects
OT  - Apoptosis
OT  - Breast cancer
OT  - DNA damage
OT  - DNA repair
OT  - ERCC1
OT  - Radiotherapy.
EDAT- 2018/04/11 06:00
MHDA- 2018/09/12 06:00
CRDT- 2018/04/11 06:00
PHST- 2017/11/02 00:00 [received]
PHST- 2018/02/12 00:00 [accepted]
PHST- 2018/02/16 00:00 [revised]
PHST- 2018/04/11 06:00 [entrez]
PHST- 2018/04/11 06:00 [pubmed]
PHST- 2018/09/12 06:00 [medline]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2018 Mar 31;64(4):64-70.