PMID- 29632138
OWN - NLM
STAT- MEDLINE
DCOM- 20190701
LR  - 20220129
IS  - 1470-8736 (Electronic)
IS  - 0143-5221 (Print)
IS  - 0143-5221 (Linking)
VI  - 132
IP  - 12
DP  - 2018 Jun 29
TI  - Effects of dietary salt on gene and protein expression in brain tissue of a model 
      of sporadic small vessel disease.
PG  - 1315-1328
LID - 10.1042/CS20171572 [doi]
AB  - BACKGROUND: The effect of salt on cerebral small vessel disease (SVD) is poorly 
      understood. We assessed the effect of dietary salt on cerebral tissue of the 
      stroke-prone spontaneously hypertensive rat (SHRSP) - a relevant model of 
      sporadic SVD - at both the gene and protein level. Methods: Brains from 
      21-week-old SHRSP and Wistar-Kyoto rats, half additionally salt-loaded (via a 
      3-week regime of 1% NaCl in drinking water), were split into two hemispheres and 
      sectioned coronally - one hemisphere for mRNA microarray and qRT-PCR, the other 
      for immunohistochemistry using a panel of antibodies targeting components of the 
      neurovascular unit. Results: We observed differences in gene and protein 
      expression affecting the acute phase pathway and oxidative stress (ALB, AMBP, 
      APOH, AHSG and LOC100129193, up-regulated in salt-loaded WKY versus WKY, 
      >2-fold), active microglia (increased Iba-1 protein expression in salt-loaded 
      SHRSP versus salt-loaded WKY, p<0.05), vascular structure (ACTB and CTNNB, 
      up-regulated in salt-loaded SHRSP versus SHRSP, >3-fold; CLDN-11, VEGF and VGF 
      down-regulated >2-fold in salt-loaded SHRSP versus SHRSP) and myelin integrity 
      (MBP down-regulated in salt loaded WKY rats versus WKY, >2.5-fold). Changes of 
      salt-loading were more pronounced in SHRSP and occurred without an increase in 
      blood pressure in WKY rats. CONCLUSION: Salt exposure induced changes in gene and 
      protein expression in an experimental model of SVD and its parent rat strain in 
      multiple pathways involving components of the glio-vascular unit. Further studies 
      in pertinent experimental models at different ages would help clarify the short- 
      and long-term effect of dietary salt in SVD.
CI  - (c) 2018 The Author(s).
FAU - Bailey, Emma L
AU  - Bailey EL
AD  - School of Life Sciences, Thomson Building, University of Glasgow, University 
      Avenue, Glasgow G12 8QQ, U.K.
FAU - McBride, Martin W
AU  - McBride MW
AD  - Institute of Cardiovascular and Medical Sciences, University of Glasgow, 
      University Avenue, Glasgow G12 8QQ, U.K.
FAU - McClure, John D
AU  - McClure JD
AD  - Institute of Cardiovascular and Medical Sciences, University of Glasgow, 
      University Avenue, Glasgow G12 8QQ, U.K.
FAU - Beattie, Wendy
AU  - Beattie W
AD  - Institute of Cardiovascular and Medical Sciences, University of Glasgow, 
      University Avenue, Glasgow G12 8QQ, U.K.
FAU - Graham, Delyth
AU  - Graham D
AD  - Institute of Cardiovascular and Medical Sciences, University of Glasgow, 
      University Avenue, Glasgow G12 8QQ, U.K.
FAU - Dominiczak, Anna F
AU  - Dominiczak AF
AD  - Institute of Cardiovascular and Medical Sciences, University of Glasgow, 
      University Avenue, Glasgow G12 8QQ, U.K.
FAU - Smith, Colin
AU  - Smith C
AD  - Academic Department of Neuropathology, Centre for Clinical Brain Sciences, Little 
      France, Edinburgh EH16 4SB, U.K.
FAU - Wardlaw, Joanna M
AU  - Wardlaw JM
AD  - Centre for Clinical Brain Sciences, and UK Dementia Research Institute at the 
      University of Edinburgh, Chancellors Building, 49 Little France Crescent, 
      Edinburgh EH16 4SB, U.K. joanna.wardlaw@ed.ac.uk.
LA  - eng
GR  - CZB/4/517/CSO_/Chief Scientist Office/United Kingdom
GR  - MR/L023784/1/MRC_/Medical Research Council/United Kingdom
GR  - RG/07/005/British Heart Foundation/United Kingdom
GR  - CH98001/British Heart Foundation/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180626
PL  - England
TA  - Clin Sci (Lond)
JT  - Clinical science (London, England : 1979)
JID - 7905731
RN  - 0 (Sodium Chloride, Dietary)
SB  - IM
MH  - Animals
MH  - Blood Pressure/drug effects
MH  - Brain/*metabolism
MH  - Cerebral Small Vessel Diseases/*metabolism
MH  - Disease Models, Animal
MH  - Gene Expression Regulation/*drug effects
MH  - Male
MH  - Oxidative Stress
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Sodium Chloride, Dietary/*pharmacology
MH  - Up-Regulation/drug effects
PMC - PMC6365623
OTO - NOTNLM
OT  - SHRSP
OT  - Salt
OT  - Small Vessel
OT  - White Matter
OT  - stroke
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2018/04/11 06:00
MHDA- 2019/07/02 06:00
PMCR- 2018/06/26
CRDT- 2018/04/11 06:00
PHST- 2017/11/28 00:00 [received]
PHST- 2018/03/13 00:00 [revised]
PHST- 2018/03/14 00:00 [accepted]
PHST- 2018/04/11 06:00 [pubmed]
PHST- 2019/07/02 06:00 [medline]
PHST- 2018/04/11 06:00 [entrez]
PHST- 2018/06/26 00:00 [pmc-release]
AID - CS20171572 [pii]
AID - 10.1042/CS20171572 [doi]
PST - epublish
SO  - Clin Sci (Lond). 2018 Jun 26;132(12):1315-1328. doi: 10.1042/CS20171572. Print 
      2018 Jun 29.