PMID- 29633064
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 70
IP  - 4
DP  - 2018 Aug
TI  - Expression profile of long non-coding RNAs during the differentiation of human 
      umbilical cord derived mesenchymal stem cells into cardiomyocyte-like cells.
PG  - 1247-1260
LID - 10.1007/s10616-018-0217-5 [doi]
AB  - We aimed to investigate the differentially expressed long non-coding RNAs 
      (lncRNAs) during the differentiation of human umbilical cord derived mesenchymal 
      stem cells (hUCMSCs) into cardiomyocyte-like cells induced by 5-aza. hUCMSCs were 
      isolated and purified from umbilical cords. After treated with 10 mumol/L 5-Aza 
      for 24 h, hUCMSCs wereas continued to be cultured for 14 days. Comparison of 
      cardiac specific genes and the expression profile of lncRNAs on hUCMSCs between 
      day 14 and day 0 was performed using immunofluorescence staining, 
      immunohistochemistry, Western blot assay, RT-PCR and lncRNA microarray. Results 
      show that well-organized sarcomeric structure and more cTnI and MLC2a staining 
      were seen in hUCMSCs of day 14 after 5-aza-induced compared to those in day 0. 
      Expression of Desmin, Nkx2.5, cTnI and MLC2a of hUCMSCs was much higher on day 14 
      compared with day 0 (P < 0.01). 41 differentially expressed lncRNAs were found on 
      day 14 hUCMSCs compared those of day 0 were identified. Among them, 25 
      upregulated and 16 downregulated. Four out of the five upregulated lncRNAs 
      (P = 0.00035, 0.014, 0.016 and 0.005 for uc010vei.1, X72487, BC064139, AK092074) 
      and four out of the five downregulated lncRNAs (P = 0.038, 0.0014, 0.00026 and 
      0.004 for X85157, uc007keu.1, AK309872, NR_029399) showed significantly different 
      expressions in further validation using RT-PCR. Our results illustrated that 
      there was a dysregulation of the lncRNA profile during the differentiation of 
      hUCMSCs into cardiomyocyte-like cells, which will provide the foundation for 
      further study of the biological functions and mechanism of lncRNAs in the 
      differentiation of hUCMSCs into cardiomyocyte-like cells.
FAU - Ruan, Zhong-Bao
AU  - Ruan ZB
AD  - Department of Cardiology, Jiangsu Taizhou People's Hospital, Taizhou, 225300, 
      People's Republic of China. tzcardiac@163.com.
FAU - Chen, Ge-Cai
AU  - Chen GC
AD  - Department of Cardiology, Jiangsu Taizhou People's Hospital, Taizhou, 225300, 
      People's Republic of China.
FAU - Ren, Yin
AU  - Ren Y
AD  - Department of Cardiology, Jiangsu Taizhou People's Hospital, Taizhou, 225300, 
      People's Republic of China.
FAU - Zhu, Li
AU  - Zhu L
AD  - Department of Cardiology, Jiangsu Taizhou People's Hospital, Taizhou, 225300, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20180409
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC6081922
OTO - NOTNLM
OT  - Cardiomyocyte-like cells
OT  - Differentiation
OT  - Expression profile
OT  - Human umbilical cord derived mesenchymal stem cells
OT  - Long non-coding RNAs
COIS- CONFLICT OF INTEREST: The authors have no conflicts of interest. ETHICS APPROVAL 
      AND CONSENT TO PARTICIPATE: This study is approved by institutional ethics 
      committee of Jiangsu Taizhou People's Hospital and written informed consent was 
      obtained from all patients.
EDAT- 2018/04/11 06:00
MHDA- 2018/04/11 06:01
PMCR- 2019/08/01
CRDT- 2018/04/11 06:00
PHST- 2018/01/14 00:00 [received]
PHST- 2018/03/30 00:00 [accepted]
PHST- 2018/04/11 06:00 [pubmed]
PHST- 2018/04/11 06:01 [medline]
PHST- 2018/04/11 06:00 [entrez]
PHST- 2019/08/01 00:00 [pmc-release]
AID - 10.1007/s10616-018-0217-5 [pii]
AID - 217 [pii]
AID - 10.1007/s10616-018-0217-5 [doi]
PST - ppublish
SO  - Cytotechnology. 2018 Aug;70(4):1247-1260. doi: 10.1007/s10616-018-0217-5. Epub 
      2018 Apr 9.