PMID- 29635137
OWN - NLM
STAT- MEDLINE
DCOM- 20181001
LR  - 20181001
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 167
DP  - 2018 Jul 1
TI  - Improvements in the water dispersibility of paclitaxel by complexing with 
      synthetic peptides derived from beta-casein.
PG  - 144-149
LID - S0927-7765(18)30190-5 [pii]
LID - 10.1016/j.colsurfb.2018.03.040 [doi]
AB  - Recently, digestive peptides prepared as a casein hydrolysate have been found to 
      be an effective dispersant for the poorly water-soluble drug paclitaxel (Ptx). A 
      major hydrophobic peptide in the digested peptides was identified as 
      YQEPVLGPVRGPFPIIV (PepY) by matrix-assisted laser desorption/ionization 
      time-of-flight/time-of-flight mass spectrometry with the "LIFT" technique. In the 
      present study, three peptides PepY, VVVPPFLQPEVMGVSKV (PepV), and 
      KFQSEEQQQTEDELQDK (PepK) were chemically synthesized by Fmoc solid-phase 
      synthesis to compare their function as dispersants for Ptx. PepV and PepK are the 
      most hydrophobic and hydrophilic peptides, respectively, in the sequence of 
      beta-casein, which are the same length as PepY (PepY, PepV, and PepK are abbreviated 
      as Peps). The complex between Ptx and Peps (Ptx-Peps) was prepared by mixing an 
      ethanol solution of Ptx and an aqueous solution of Peps, followed by 
      lyophilization. The complex with PepV, which is estimated to be the most 
      hydrophobic of the peptides, had the greatest ability to improve the water 
      dispersibility of Ptx. The water dispersibility of the complexes between Ptx and 
      PepY and PepV increased as the amount of the peptides increased, whereas PepK was 
      not effective in enhancing the dispersibility of Ptx. Furthermore, a peptide 
      mixture obtained from a casein hydrolysate [Pep (fraction A)] was more effective 
      for the enhancement of Ptx dispersibility than the single peptide PepY. These 
      results suggests that a variety of peptides in the casein hydrolysate contribute 
      toward complexation with Ptx.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Inada, Asuka
AU  - Inada A
AD  - Center for Membrane and Film Technology, Department of Chemical Science and 
      Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, 
      Japan. Electronic address: a.inada@port.kobe-u.ac.jp.
FAU - Sakurai, Yuki
AU  - Sakurai Y
AD  - Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 
      1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan. Electronic address: 
      hg13022@student.miyazaki-u.ac.jp.
FAU - Oshima, Tatsuya
AU  - Oshima T
AD  - Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 
      1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan. Electronic address: 
      oshimat@cc.miyazaki-u.ac.jp.
FAU - Baba, Yoshinari
AU  - Baba Y
AD  - Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 
      1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan. Electronic address: 
      t0g202u@cc.miyazaki-u.ac.jp.
FAU - Matsuyama, Hideto
AU  - Matsuyama H
AD  - Center for Membrane and Film Technology, Department of Chemical Science and 
      Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, 
      Japan. Electronic address: matuyama@kobe-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20180329
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Caseins)
RN  - 0 (Peptides)
RN  - 059QF0KO0R (Water)
RN  - 65072-00-6 (casein hydrolysate)
RN  - P88XT4IS4D (Paclitaxel)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antineoplastic Agents, Phytogenic/chemistry
MH  - Caseins/*chemistry
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Paclitaxel/*chemistry
MH  - Particle Size
MH  - Peptides/chemical synthesis/*chemistry
MH  - Solubility
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Water/*chemistry
OTO - NOTNLM
OT  - Casein hydrolysate
OT  - Complex
OT  - Dispersibility
OT  - Paclitaxel
OT  - Synthetic peptide
EDAT- 2018/04/11 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/04/11 06:00
PHST- 2017/12/05 00:00 [received]
PHST- 2018/03/22 00:00 [revised]
PHST- 2018/03/24 00:00 [accepted]
PHST- 2018/04/11 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/04/11 06:00 [entrez]
AID - S0927-7765(18)30190-5 [pii]
AID - 10.1016/j.colsurfb.2018.03.040 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2018 Jul 1;167:144-149. doi: 
      10.1016/j.colsurfb.2018.03.040. Epub 2018 Mar 29.