PMID- 29635716
OWN - NLM
STAT- MEDLINE
DCOM- 20191007
LR  - 20191007
IS  - 1520-6777 (Electronic)
IS  - 0733-2467 (Linking)
VI  - 37
IP  - 7
DP  - 2018 Sep
TI  - Beta-3 adrenoceptor expression in the uterosacral ligament in the postmenopausal 
      women with pelvic organ prolapse.
PG  - 2135-2140
LID - 10.1002/nau.23579 [doi]
AB  - AIMS: beta3-adrenoceptor (beta3-AR) agonist is known to relax the detrusor muscles. 
      Considering that both pelvic organ prolapse (POP) and overactive-bladder (OAB) 
      are frequently seen in the elderly population, the effect of beta3-AR agonist on the 
      pelvic floor tissues should be investigated. As a first step, this pilot, 
      experimental, and descriptive study was designed to investigate the presence of 
      beta3-AR expression in the uterosacral-ligaments (USLs) in postmenopausal (PMP) 
      women with POP. METHODS: After informed consent, PMP women undergoing 
      hysterectomy for POP were recruited for the intraoperative collection of distal 
      USL between 5/2016 and 8/2016. H&E analysis for the USLs and immunohistochemistry 
      (IHC) analysis for beta3-AR and alpha-actin smooth muscle (SM) were carried out. Tissue 
      composition and percentage of beta3-AR expression in the USLs were subjectively 
      measured and the staining results were expressed as descriptive analysis. 
      RESULTS: Total 11 USL specimens were collected and nine specimens were included 
      for the final analysis (one specimen: used for antibody concentration adjustment 
      and one specimen: failed proper staining). Under high-power-field, the USLs were 
      composed of SM cells (81.5 +/- 7.47%) and connective tissues (16.5 +/- 7.9%). On IHC 
      analysis, six out of nine specimens expressed beta3-AR with a different level of 
      expression in SM cells in the USLs. CONCLUSIONS: The majority of distal USLs were 
      composed of SM cells. beta3-ARs are expressed in 67% of the study specimens. 
      Considering that both POP and OAB are frequently seen in the elderly population, 
      the effect of beta3-AR agonist on the pelvic floor tissues should be investigated 
      further.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Chong, Woojin
AU  - Chong W
AUID- ORCID: 0000-0001-5388-1049
AD  - Obstetrics, Gynecology and Reproductive Sciences, Mount Sinai Medical 
      Center/Icahn School of Medicine, New York, New York.
FAU - Fantl, John Andrew
AU  - Fantl JA
AD  - Obstetrics, Gynecology and Reproductive Sciences, Mount Sinai Medical 
      Center/Icahn School of Medicine, New York, New York.
FAU - Donovan, Michael
AU  - Donovan M
AD  - Anatomic Pathology, Mount Sinai Medical Center/Icahn School of Medicine, New 
      York, New York.
FAU - Ascher-Walsh, Charles
AU  - Ascher-Walsh C
AD  - Obstetrics, Gynecology and Reproductive Sciences, Mount Sinai Medical 
      Center/Icahn School of Medicine, New York, New York.
LA  - eng
GR  - #0244-1520/Mount Sinai Medical Center/Icahn School of Medicine/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180410
PL  - United States
TA  - Neurourol Urodyn
JT  - Neurourology and urodynamics
JID - 8303326
RN  - 0 (Actins)
RN  - 0 (Receptors, Adrenergic, beta-3)
SB  - IM
MH  - Actins/metabolism
MH  - Aged
MH  - Connective Tissue/metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Ligaments/*metabolism
MH  - Middle Aged
MH  - Myocytes, Smooth Muscle/metabolism
MH  - Pelvic Organ Prolapse/*metabolism
MH  - Pilot Projects
MH  - Postmenopause
MH  - Receptors, Adrenergic, beta-3/*metabolism
MH  - *Sacrococcygeal Region
MH  - Uterus/*metabolism
OTO - NOTNLM
OT  - overactive bladder
OT  - pelvic organ prolapse
OT  - uterosacral ligaments
OT  - beta3-adrenoceptor
EDAT- 2018/04/11 06:00
MHDA- 2019/10/08 06:00
CRDT- 2018/04/11 06:00
PHST- 2018/01/02 00:00 [received]
PHST- 2018/03/06 00:00 [accepted]
PHST- 2018/04/11 06:00 [pubmed]
PHST- 2019/10/08 06:00 [medline]
PHST- 2018/04/11 06:00 [entrez]
AID - 10.1002/nau.23579 [doi]
PST - ppublish
SO  - Neurourol Urodyn. 2018 Sep;37(7):2135-2140. doi: 10.1002/nau.23579. Epub 2018 Apr 
      10.