PMID- 29636867 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220330 IS - 1943-8141 (Print) IS - 1943-8141 (Electronic) IS - 1943-8141 (Linking) VI - 10 IP - 3 DP - 2018 TI - Microarray expression profiling and bioinformatics analysis of circular RNA expression in lung squamous cell carcinoma. PG - 771-783 AB - Circular RNAs (circRNAs) are novel noncoding RNAs with a wide range of physiological and pathological activities. However, the expression profile and roles in lung squamous cell carcinoma (LSCC) remain largely unknown. Therefore, we investigated the expression profile of circRNAs in three LSCC and matched adjacent normal tissues using microarray. Total 216 differentially expressed circRNAs were identified, including 135 upregulated and 81 downregulated ones in LSCC tissues. Bioinformatics analysis revealed that these differentially expressed circRNAs were potentially implicated in carcinogenesis using Gene ontology (GO) and KEGG pathway analyses. By constructing miRNA-circRNA interaction network, a total of ten key circRNAs, including 6 upregulated and 4 downregulated circRNAs were further screened and then confirmed using qRT-PCR analysis in another 40 paired of LSCC tissues and adjacent normal tissues. In addition, Kaplan-Meier survival analysis demonstrated that the overall survival time of LSCC patients with high hsa_circRNA_103827 expression and low hsa_circRNA_000122 was significantly shorter (P<0.001). In conclusion, this study provides evidence that circRNAs are differentially expressed in LSCC and closely related to the carcinogenesis of LSCC. Among these, hsa_circRNA_103827 and hsa_circRNA_000122 might be served as potential prognostic biomarkers and therapeutic target for LSCC. FAU - Xu, Jiali AU - Xu J AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Shu, Yongqian AU - Shu Y AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Xu, Tongpeng AU - Xu T AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Zhu, Wei AU - Zhu W AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Qiu, Tianzhu AU - Qiu T AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Li, Jun AU - Li J AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Zhang, Meiling AU - Zhang M AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Xu, Jing AU - Xu J AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Guo, Renhua AU - Guo R AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Lu, Kaihua AU - Lu K AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Zhu, Lingjun AU - Zhu L AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Yin, Yongmei AU - Yin Y AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Gu, Yanhong AU - Gu Y AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Liu, Lianke AU - Liu L AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Liu, Ping AU - Liu P AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. FAU - Wang, Rong AU - Wang R AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province HospitalNo. 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, People's Republic of China. LA - eng PT - Journal Article DEP - 20180315 PL - United States TA - Am J Transl Res JT - American journal of translational research JID - 101493030 PMC - PMC5883118 OTO - NOTNLM OT - Lung squamous cell carcinoma OT - biomarker OT - circRNA OT - circRNA-miRNA network OT - microarray analysis COIS- None. EDAT- 2018/04/11 06:00 MHDA- 2018/04/11 06:01 PMCR- 2018/03/15 CRDT- 2018/04/12 06:00 PHST- 2018/01/02 00:00 [received] PHST- 2018/02/02 00:00 [accepted] PHST- 2018/04/12 06:00 [entrez] PHST- 2018/04/11 06:00 [pubmed] PHST- 2018/04/11 06:01 [medline] PHST- 2018/03/15 00:00 [pmc-release] PST - epublish SO - Am J Transl Res. 2018 Mar 15;10(3):771-783. eCollection 2018.