PMID- 29637046
OWN - NLM
STAT- MEDLINE
DCOM- 20190410
LR  - 20240322
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 8
DP  - 2018
TI  - Endotyping of Chronic Rhinosinusitis With and Without Polyp Using Transcription 
      Factor Analysis.
PG  - 82
LID - 10.3389/fcimb.2018.00082 [doi]
LID - 82
AB  - Inflammation of the nose and paranasal sinus or rhinosinusitis (RS) is a 
      significant global health problem that is both very common and very costly to 
      treat. Previous reports reveal variability in histology and mechanism of 
      inflammation in patients with chronic rhinosinusitis with and without polyp 
      (CRScNP and CRSsNP, respectively). There are various methods and hypothesis that 
      try to explain this variability. Accordingly, the aim of this study was to 
      investigate the incidence of each type of sinonasal inflammation among patients 
      diagnosed with CRScNP or CRSsNP using transcription factor analysis (TFA). This 
      study included mucosa specimens from nose/paranasal sinuses from patients with 
      chronic rhinitis (CR), CRSsNP, or CRScNP that were obtained at the Department of 
      Otorhinolaryngology, Faculty of Medicine Siriraj Hospital, Mahidol University, 
      Bangkok, Thailand during the June 2009 to May 2012 study period. TFA was employed 
      to measure the following transcription factors: T-box transcription factor 
      (T-bet) for Th1, GATA binding protein 3 (GATA-3) for Th2, retinoic acid-related 
      orphan receptor C (RORC) for Th17, and forkhead box P3 (FOXP3) for Treg. 
      Forty-one subjects (22 males, 19 females) were enrolled, with a mean age of 45.93 
      +/- 13 years. Twenty-six patients were diagnosed with CRScNP, 7 with CRSsNP, and 8 
      with CR (controls). The majority of CRScNP specimens (76.9%) had eosinophil count 
      greater than 100 cells/high-power field (HPF). Mean eosinophil count was 930.08 +/- 
      1,399 cells/HPF (range: 17-5,570). Th2 transcription factor (GATA-3) was 
      statistically significantly higher in the CRScNP group than in the CRS and 
      control groups (p < 0.001); whereas, Treg transcription factor (FOXP3) was 
      statistically significantly lower in the CRScNP group than in the CRSsNP and 
      control groups (p < 0.001). The transcription factors for Th1 and Th17 (T-bet and 
      RORC, respectively) were not significantly different among the three groups. The 
      result of transcription factor analysis revealed hyperfunction of Th2 in patients 
      with CRScNP, which might result in hypereosinophilic infliltration in the polyps. 
      One explanation for this finding is the decreased activity of Treg. Although 
      environment-host interaction is the most probable hypothesis, the etiology of 
      aberrant adaptive immunity needs to be elucidated.
FAU - Tantilipikorn, Pongsakorn
AU  - Tantilipikorn P
AD  - Division of Rhinology and Allergy, Department of Otorhinolaryngology, Faculty of 
      Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Sookrung, Nitat
AU  - Sookrung N
AD  - Office of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, Bangkok, Thailand.
FAU - Muangsomboon, Soranart
AU  - Muangsomboon S
AD  - Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, Bangkok, Thailand.
FAU - Lumyongsatien, Jate
AU  - Lumyongsatien J
AD  - Division of Rhinology and Allergy, Department of Otorhinolaryngology, Faculty of 
      Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Bedavanija, Anan
AU  - Bedavanija A
AD  - Division of Rhinology and Allergy, Department of Otorhinolaryngology, Faculty of 
      Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Suwanwech, Triphoom
AU  - Suwanwech T
AD  - Division of Rhinology and Allergy, Department of Otorhinolaryngology, Faculty of 
      Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180327
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adult
MH  - Chronic Disease
MH  - Female
MH  - Gene Expression Profiling
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polyps/genetics/immunology/pathology
MH  - Rhinitis/psychology
MH  - Sinusitis/*genetics/immunology/pathology
MH  - Th1 Cells/immunology
MH  - Th17 Cells/immunology
MH  - Thailand
MH  - Transcription Factors/*genetics/immunology
MH  - Young Adult
PMC - PMC5880998
OTO - NOTNLM
OT  - Thailand
OT  - chronic rhinitis
OT  - chronic rhinosinusitis
OT  - endotyping
OT  - polyp
OT  - transcription factor analysis
EDAT- 2018/04/11 06:00
MHDA- 2019/04/11 06:00
PMCR- 2018/01/01
CRDT- 2018/04/12 06:00
PHST- 2017/11/06 00:00 [received]
PHST- 2018/02/28 00:00 [accepted]
PHST- 2018/04/12 06:00 [entrez]
PHST- 2018/04/11 06:00 [pubmed]
PHST- 2019/04/11 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2018.00082 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2018 Mar 27;8:82. doi: 10.3389/fcimb.2018.00082. 
      eCollection 2018.