PMID- 29641231
OWN - NLM
STAT- MEDLINE
DCOM- 20190715
LR  - 20240312
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 315
IP  - 2
DP  - 2018 Aug 1
TI  - A role for the CXCR4-CXCL12 axis in the little skate, Leucoraja erinacea.
PG  - R218-R229
LID - 10.1152/ajpregu.00322.2017 [doi]
AB  - The interaction between C-X-C chemokine receptor type 4 (CXCR4) and its cognate 
      ligand C-X-C motif chemokine ligand 12 (CXCL12) plays a critical role in 
      regulating hematopoietic stem cell activation and subsequent cellular 
      mobilization. Extensive studies of these genes have been conducted in mammals, 
      but much less is known about the expression and function of CXCR4 and CXCL12 in 
      non-mammalian vertebrates. In the present study, we identify simultaneous 
      expression of CXCR4 and CXCL12 orthologs in the epigonal organ (the primary 
      hematopoietic tissue) of the little skate, Leucoraja erinacea. Genetic and 
      phylogenetic analyses were functionally supported by significant mobilization of 
      leukocytes following administration of Plerixafor, a CXCR4 antagonist and 
      clinically important drug. Our results provide evidence that, as in humans, 
      Plerixafor disrupts CXCR4/CXCL12 binding in the little skate, facilitating 
      release of leukocytes into the bloodstream. Our study illustrates the value of 
      the little skate as a model organism, particularly in studies of hematopoiesis 
      and potentially for preclinical research on hematological and vascular disorders.
FAU - Hersh, Taylor A
AU  - Hersh TA
AUID- ORCID: 0000-0002-7891-596X
AD  - Mount Desert Island Biological Laboratory , Bar Harbor, Maine.
FAU - Dimond, Alexandria L
AU  - Dimond AL
AD  - Mount Desert Island Biological Laboratory , Bar Harbor, Maine.
AD  - School of Arts and Sciences, Endicott College , Beverly, Massachusetts.
FAU - Ruth, Brittany A
AU  - Ruth BA
AD  - Mount Desert Island Biological Laboratory , Bar Harbor, Maine.
AD  - School of Arts and Sciences, Endicott College , Beverly, Massachusetts.
FAU - Lupica, Noah V
AU  - Lupica NV
AD  - Mount Desert Island Biological Laboratory , Bar Harbor, Maine.
FAU - Bruce, Jacob C
AU  - Bruce JC
AD  - Mount Desert Island Biological Laboratory , Bar Harbor, Maine.
FAU - Kelley, John M
AU  - Kelley JM
AD  - School of Arts and Sciences, Endicott College , Beverly, Massachusetts.
AD  - Beth Israel Deaconess Medical Center, Program in Placebo Studies, Harvard Medical 
      School , Boston, Massachusetts.
FAU - King, Benjamin L
AU  - King BL
AD  - Department of Molecular and Biomedical Sciences, University of Maine , Orono, 
      Maine.
FAU - Lutton, Bram V
AU  - Lutton BV
AD  - Mount Desert Island Biological Laboratory , Bar Harbor, Maine.
AD  - School of Arts and Sciences, Endicott College , Beverly, Massachusetts.
LA  - eng
GR  - P20 GM103423/GM/NIGMS NIH HHS/United States
GR  - P20 GM104318/GM/NIGMS NIH HHS/United States
GR  - R25 ES016254/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20180411
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Benzylamines)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cyclams)
RN  - 0 (Fish Proteins)
RN  - 0 (Heterocyclic Compounds)
RN  - 0 (Receptors, CXCR4)
RN  - S915P5499N (plerixafor)
SB  - IM
MH  - Animals
MH  - Benzylamines
MH  - Chemokine CXCL12/genetics/*metabolism
MH  - Cyclams
MH  - Fish Proteins/genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Hematopoietic Stem Cells/drug effects/*metabolism
MH  - Heterocyclic Compounds/pharmacology
MH  - Leukocytes/drug effects/*metabolism
MH  - *Leukopoiesis/drug effects/genetics
MH  - Phylogeny
MH  - Receptors, CXCR4/antagonists & inhibitors/genetics/*metabolism
MH  - Signal Transduction
MH  - Skates, Fish/genetics/*metabolism
MH  - Transcriptome
PMC - PMC6139610
OTO - NOTNLM
OT  - C-X-C chemokine ligand 12
OT  - C-X-C chemokine receptor type 4
OT  - Plerixafor/AMD3100
OT  - elasmobranch
OT  - mobilization
EDAT- 2018/04/12 06:00
MHDA- 2019/07/16 06:00
PMCR- 2019/08/01
CRDT- 2018/04/12 06:00
PHST- 2018/04/12 06:00 [pubmed]
PHST- 2019/07/16 06:00 [medline]
PHST- 2018/04/12 06:00 [entrez]
PHST- 2019/08/01 00:00 [pmc-release]
AID - R-00322-2017 [pii]
AID - 10.1152/ajpregu.00322.2017 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2018 Aug 1;315(2):R218-R229. doi: 
      10.1152/ajpregu.00322.2017. Epub 2018 Apr 11.