PMID- 29642953 OWN - NLM STAT- MEDLINE DCOM- 20190110 LR - 20230816 IS - 1757-6512 (Electronic) IS - 1757-6512 (Linking) VI - 9 IP - 1 DP - 2018 Apr 11 TI - Impact of bone marrow mesenchymal stem cell immunomodulation on the osteogenic effects of laponite. PG - 100 LID - 10.1186/s13287-018-0818-0 [doi] LID - 100 AB - BACKGROUND: With the development of osteoimmunology and bone tissue engineering (BTE), it has been recognized that the immunomodulatory properties of bone biomaterials have considerable impact in determining their fate after implantation. In this regard, the polarization of macrophages secondary to biomaterials is postulated to play a crucial role in modulating their osteogenesis; thus, strategies that may facilitate this process engender increasing levels of attention. Whereas a variety of reports highlight the immunomodulation of bone marrow mesenchymal stem cells (BMSCs) in cell therapy or their osteogenesis in BTE, few have focused on the effect of BMSCs in promoting osteogenesis in BTE through regulating the phenotype of macrophages. Accordingly, there is an urgent need to clarify the immunomodulatory properties of agents such as laponite (Lap), which is comprised of bioactive silicate nanoplatelets with excellent osteogenesis-inducing potential, to enhance their use in BTE. METHODS: In the present study, we analyzed the osteoimmunomodulatory properties of Lap alone, as well as following the introduction of BMSCs into Lap, to determine whether BMSCs could modulate its immunomodulatory properties and promote osteogenesis. RESULTS: It was found that the BMSCs reversed the polarization of murine-derived macrophage RAW 264.7 cells from M1 as induced by pure Lap to M2 and promoted osteogenesis. In vivo study confirmed that BMSCs combined with Lap initiated a less severe immune response and had an improved effect on bone regeneration compared with Lap alone, which corresponded with the in vitro evaluation. CONCLUSION: These results suggest that BMSCs could ameliorate the inflammation induced by Lap and enhance its bone formation. The immunomodulatory characteristics of BMSCs suggest that these might be tailored as a new strategy to promote the osteogenic capacity of biomaterials. FAU - Li, Tao AU - Li T AD - Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Room 701, No. 3 Building, 639 Zhizaoju Road, Shanghai, 200011, People's Republic of China. FAU - Liu, Zhong Long AU - Liu ZL AD - Department of Oral Maxillofacial & Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China. FAU - Xiao, Ming AU - Xiao M AD - Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Room 701, No. 3 Building, 639 Zhizaoju Road, Shanghai, 200011, People's Republic of China. FAU - Yang, Ze Zheng AU - Yang ZZ AD - Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Room 701, No. 3 Building, 639 Zhizaoju Road, Shanghai, 200011, People's Republic of China. FAU - Peng, Ming Zheng AU - Peng MZ AD - Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Room 701, No. 3 Building, 639 Zhizaoju Road, Shanghai, 200011, People's Republic of China. FAU - Li, Cui Di AU - Li CD AD - School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China. FAU - Zhou, Xiao Jun AU - Zhou XJ AD - Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Room 701, No. 3 Building, 639 Zhizaoju Road, Shanghai, 200011, People's Republic of China. xjz362@sina.com. FAU - Wang, Jin Wu AU - Wang JW AD - Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Room 701, No. 3 Building, 639 Zhizaoju Road, Shanghai, 200011, People's Republic of China. jinwuwang123@sina.com. LA - eng GR - R01 AA020308/AA/NIAAA NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180411 PL - England TA - Stem Cell Res Ther JT - Stem cell research & therapy JID - 101527581 RN - 0 (Silicates) RN - D703131383 (laponite) SB - IM MH - Animals MH - Bone Regeneration MH - Cell Line MH - Cells, Cultured MH - Female MH - Guided Tissue Regeneration/methods MH - *Immunomodulation MH - Macrophages/*immunology MH - Mesenchymal Stem Cells/*drug effects/immunology MH - Mice MH - *Osteogenesis MH - Rats MH - Rats, Sprague-Dawley MH - Silicates/*pharmacology PMC - PMC5896058 OTO - NOTNLM OT - Bone marrow mesenchymal stem cell OT - Immunomodulation OT - Laponite OT - Macrophage OT - Osteogenesis COIS- ETHICS APPROVAL: All animal procedures were approved by the Animal Experimental Ethic Committee of the Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine (HKDL [2016]56), and the experimental procedures were in accordance with the Intramural Animal Use and Care Committee of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2018/04/13 06:00 MHDA- 2019/01/11 06:00 PMCR- 2018/04/11 CRDT- 2018/04/13 06:00 PHST- 2017/10/06 00:00 [received] PHST- 2018/02/26 00:00 [accepted] PHST- 2018/01/30 00:00 [revised] PHST- 2018/04/13 06:00 [entrez] PHST- 2018/04/13 06:00 [pubmed] PHST- 2019/01/11 06:00 [medline] PHST- 2018/04/11 00:00 [pmc-release] AID - 10.1186/s13287-018-0818-0 [pii] AID - 818 [pii] AID - 10.1186/s13287-018-0818-0 [doi] PST - epublish SO - Stem Cell Res Ther. 2018 Apr 11;9(1):100. doi: 10.1186/s13287-018-0818-0.