PMID- 29645353
OWN - NLM
STAT- MEDLINE
DCOM- 20190626
LR  - 20190626
IS  - 1744-7917 (Electronic)
IS  - 1672-9609 (Linking)
VI  - 26
IP  - 5
DP  - 2019 Oct
TI  - Discovering genes responsible for silk synthesis in Bombyx mori by piggyBac-based 
      random insertional mutagenesis.
PG  - 821-830
LID - 10.1111/1744-7917.12595 [doi]
AB  - Silkworm mutants are valuable resources for both transgenic breeding and gene 
      discovery. PiggyBac-based random insertional mutagenesis has been widely used in 
      gene functional studies. In order to discover genes involved in silk synthesis, a 
      piggyBac-based random insertional library was constructed using Bombyx mori, and 
      the mutants with abnormal cocoon were particularly screened. By this means, a 
      "thin cocoon" mutant was identified. This mutant revealed thinner cocoon shell 
      and shorter posterior silk gland (PSG) compared with the wild type. The messenger 
      RNA (mRNA) levels of all the three fibroin genes, including Fib-H, Fib-L and P25, 
      were significantly down-regulated in the PSG of mutants. Four piggyBac insertion 
      sites were identified in Aquaporin (AQP), Longitudinals lacking protein-like 
      (Lola), Glutamyl aminopeptidase-like (GluAP) and Loc101744460. The mRNA levels of 
      all the four genes were significantly altered in the silk gland of mutants. In 
      particular, the mRNA amount of AQP, a gene responsible for the regulation of 
      osmotic pressure, decreased dramatically immediately prior to the spinning stage 
      in the anterior silk gland of mutants. The identification of the genes disrupted 
      in the "thin cocoon" mutant in this study provided useful information for 
      understanding silk production and transgenic breeding of silkworms in the future.
CI  - (c) 2018 Institute of Zoology, Chinese Academy of Sciences.
FAU - Feng, Xing-Bao
AU  - Feng XB
AD  - Guangzhou Key Laboratory of Insect Development Regulation and Applied Research, 
      Institute of Insect Science and Technology, School of Life Sciences, South China 
      Normal University, Guangzhou, China.
FAU - Zheng, Zi-Wen
AU  - Zheng ZW
AD  - Guangzhou Key Laboratory of Insect Development Regulation and Applied Research, 
      Institute of Insect Science and Technology, School of Life Sciences, South China 
      Normal University, Guangzhou, China.
FAU - Zhang, Xian
AU  - Zhang X
AD  - Guangzhou Key Laboratory of Insect Development Regulation and Applied Research, 
      Institute of Insect Science and Technology, School of Life Sciences, South China 
      Normal University, Guangzhou, China.
FAU - Gu, Jun
AU  - Gu J
AD  - Guangzhou Key Laboratory of Insect Development Regulation and Applied Research, 
      Institute of Insect Science and Technology, School of Life Sciences, South China 
      Normal University, Guangzhou, China.
FAU - Feng, Qi-Li
AU  - Feng QL
AD  - Guangzhou Key Laboratory of Insect Development Regulation and Applied Research, 
      Institute of Insect Science and Technology, School of Life Sciences, South China 
      Normal University, Guangzhou, China.
FAU - Huang, Li-Hua
AU  - Huang LH
AUID- ORCID: 0000-0002-0444-8537
AD  - Guangzhou Key Laboratory of Insect Development Regulation and Applied Research, 
      Institute of Insect Science and Technology, School of Life Sciences, South China 
      Normal University, Guangzhou, China.
LA  - eng
GR  - 201510010055/Science and Technology Project of Guangzhou City/
GR  - 31672357/National Natural Science Foundation of China/
GR  - 2015A030313390/Natural Science Foundation of Guangdong Province/
PT  - Journal Article
DEP - 20180517
PL  - Australia
TA  - Insect Sci
JT  - Insect science
JID - 101266965
RN  - 0 (Insect Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Silk)
RN  - 9007-76-5 (Fibroins)
SB  - IM
MH  - Animals
MH  - Bombyx/*genetics/growth & development/metabolism
MH  - Fibroins/genetics
MH  - Insect Proteins/genetics
MH  - Larva/genetics/metabolism
MH  - Mutagenesis, Insertional/methods
MH  - RNA, Messenger
MH  - Silk/biosynthesis/*genetics
OTO - NOTNLM
OT  - Bombyx mori
OT  - aquaporin
OT  - development
OT  - mutant
OT  - piggyBac
OT  - silk gland
EDAT- 2018/04/13 06:00
MHDA- 2019/06/27 06:00
CRDT- 2018/04/13 06:00
PHST- 2017/11/22 00:00 [received]
PHST- 2018/03/25 00:00 [revised]
PHST- 2018/03/25 00:00 [accepted]
PHST- 2018/04/13 06:00 [pubmed]
PHST- 2019/06/27 06:00 [medline]
PHST- 2018/04/13 06:00 [entrez]
AID - 10.1111/1744-7917.12595 [doi]
PST - ppublish
SO  - Insect Sci. 2019 Oct;26(5):821-830. doi: 10.1111/1744-7917.12595. Epub 2018 May 
      17.