PMID- 29651154
OWN - NLM
STAT- MEDLINE
DCOM- 20191004
LR  - 20191007
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Apr 12
TI  - Effect of Concentrated Growth Factor (CGF) on the Promotion of Osteogenesis in 
      Bone Marrow Stromal Cells (BMSC) in vivo.
PG  - 5876
LID - 10.1038/s41598-018-24364-5 [doi]
LID - 5876
AB  - The therapeutic method traditionally used in bone defect reconstruction is 
      autologous bone grafting. The most common problems affecting this type of repair 
      approach are bone absorption and donor trauma. The approach taken in this study 
      overcomes these problems. Bone marrow stromal cells (BMSCs) provided the crucial 
      seed cells. Fibrin biological scaffolds were formed by combining the BMSCs with 
      concentrated growth factor (CGF). BMSCs were isolated from Wistar rat femurs; CGF 
      was prepared from rat heart blood. Five repair groups were created for 
      comparative purposes: (A) CGF + BMSCs; (B) CGF; (C) collagen + BMSCs; (D) 
      collagen; (E) blank. After three months, the rats were sacrificed, and 
      histopathology and three-dimensional CT images produced. Bone regeneration was 
      significantly higher in the (A) CGF + BMSC group; osteogenesis was lower in the 
      (B) CGF and (C) collagen + BMSC groups, at very similar levels; the (D) collagen 
      and (E) blank groups scored the lowest results. Our research suggests that 
      combining CGF with BMSCs leads to the formation of fibrin scaffolds that have a 
      powerful effect on osteogenesis as well as a subsidiary angiogenic effect. SEM 
      images of the CGF scaffolds cultured with BMSCs confirmed good CGF 
      biocompatibility. The superior osteoinductive activity of the CGF + BMSC 
      combination makes it an excellent biomaterial for bone regeneration.
FAU - Chen, Xia
AU  - Chen X
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, 
      Shanghai, 200011, China.
FAU - Wang, Jian
AU  - Wang J
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, 
      Shanghai, 200011, China. profwangjian@163.com.
FAU - Yu, Li
AU  - Yu L
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, 
      Shanghai, 200011, China. yuoli@163.com.
FAU - Zhou, Jia
AU  - Zhou J
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, 
      Shanghai, 200011, China.
FAU - Zheng, Danning
AU  - Zheng D
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, 
      Shanghai, 200011, China.
FAU - Zhang, Bo
AU  - Zhang B
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, 
      Shanghai, 200011, China.
LA  - eng
PT  - Journal Article
DEP - 20180412
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 9001-31-4 (Fibrin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Bone Regeneration/drug effects
MH  - Bone Transplantation
MH  - Cell Differentiation/*drug effects/genetics
MH  - Collagen/administration & dosage
MH  - Fibrin/chemistry/metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*administration & dosage
MH  - Mesenchymal Stem Cells/drug effects/*physiology
MH  - Osteogenesis/*drug effects
MH  - Rats
MH  - Tissue Scaffolds
PMC - PMC5897572
COIS- The authors declare no competing interests.
EDAT- 2018/04/14 06:00
MHDA- 2019/10/08 06:00
PMCR- 2018/04/12
CRDT- 2018/04/14 06:00
PHST- 2017/06/07 00:00 [received]
PHST- 2018/03/27 00:00 [accepted]
PHST- 2018/04/14 06:00 [entrez]
PHST- 2018/04/14 06:00 [pubmed]
PHST- 2019/10/08 06:00 [medline]
PHST- 2018/04/12 00:00 [pmc-release]
AID - 10.1038/s41598-018-24364-5 [pii]
AID - 24364 [pii]
AID - 10.1038/s41598-018-24364-5 [doi]
PST - epublish
SO  - Sci Rep. 2018 Apr 12;8(1):5876. doi: 10.1038/s41598-018-24364-5.