PMID- 29651654
OWN - NLM
STAT- MEDLINE
DCOM- 20190314
LR  - 20200225
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Print)
IS  - 0364-3190 (Linking)
VI  - 44
IP  - 1
DP  - 2019 Jan
TI  - In the Telencephalon, GluN2C NMDA Receptor Subunit mRNA is Predominately 
      Expressed in Glial Cells and GluN2D mRNA in Interneurons.
PG  - 61-77
LID - 10.1007/s11064-018-2526-7 [doi]
AB  - N-methyl-D-aspartate receptors (NMDARs) are widely distributed in the brain with 
      high concentrations in the telencephalon where they modulate synaptic plasticity, 
      working memory, and other functions. While the actions of the predominate GluN2 
      NMDAR subunits, GluN2A and GluN2B are relatively well understood, the function of 
      GluN2C and GluN2D subunits in the telencephalon is largely unknown. To better 
      understand the possible role of GluN2C subunits, we used fluorescence in situ 
      hybridization (FISH) together with multiple cell markers to define the 
      distribution and type of cells expressing GluN2C mRNA. Using a GluN2C-KO mouse as 
      a negative control, GluN2C mRNA expression was only found in non-neuronal cells 
      (NeuN-negative cells) in the hippocampus, striatum, amygdala, and cerebral 
      cortex. For these regions, a significant fraction of GFAP-positive cells also 
      expressed GluN2C mRNA. Overall, for the telencephalon, the globus pallidus and 
      olfactory bulb were the only regions where GluN2C was expressed in neurons. In 
      contrast to GluN2C, GluN2D subunit mRNA colocalized with neuronal and not 
      astrocyte markers or GluN2C mRNA in the telencephalon (except for the globus 
      pallidus). GluN2C mRNA did, however, colocalize with GluN2D in the thalamus where 
      neuronal GluN2C expression is found. These findings strongly suggest that GluN2C 
      has a very distinct function in the telencephalon compared to its role in other 
      brain regions and compared to other GluN2-containing NMDARs. NMDARs containing 
      GluN2C may have a specific role in regulating L-glutamate or D-serine release 
      from astrocytes in response to L-glutamate spillover from synaptic activity.
FAU - Alsaad, Hassan A
AU  - Alsaad HA
AD  - Department of Pharmacology and Experimental Neuroscience, University of Nebraska 
      Medical Center, Omaha, NE, 68198-5800, USA.
FAU - DeKorver, Nicholas W
AU  - DeKorver NW
AD  - Department of Pharmacology and Experimental Neuroscience, University of Nebraska 
      Medical Center, Omaha, NE, 68198-5800, USA.
FAU - Mao, Zhihao
AU  - Mao Z
AD  - Department of Pharmacology and Experimental Neuroscience, University of Nebraska 
      Medical Center, Omaha, NE, 68198-5800, USA.
FAU - Dravid, Shashank M
AU  - Dravid SM
AD  - Department of Pharmacology, Creighton University, Omaha, NE, USA.
FAU - Arikkath, Jyothi
AU  - Arikkath J
AD  - Department of Developmental Neuroscience, Monroe Meyer Institute, University of 
      Nebraska Medical Center, Omaha, NE, USA.
FAU - Monaghan, Daniel T
AU  - Monaghan DT
AUID- ORCID: 0000-0002-3261-1901
AD  - Department of Pharmacology and Experimental Neuroscience, University of Nebraska 
      Medical Center, Omaha, NE, 68198-5800, USA. dtmonagh@unmc.edu.
LA  - eng
GR  - P30 GM110768/GM/NIGMS NIH HHS/United States
GR  - MH60252/National Institutes of Health (US)/
GR  - R01 MH060252/MH/NIMH NIH HHS/United States
GR  - GM110768/National Institutes of Health (US)/
GR  - R21 NS104705/NS/NINDS NIH HHS/United States
PT  - Journal Article
DEP - 20180412
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (NR2C NMDA receptor)
RN  - 0 (NR2D NMDA receptor)
RN  - 0 (Protein Subunits)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Animals
MH  - Gene Expression
MH  - Interneurons/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Neuroglia/*metabolism
MH  - Protein Subunits/biosynthesis/genetics
MH  - RNA, Messenger/*biosynthesis/genetics
MH  - Receptors, N-Methyl-D-Aspartate/*biosynthesis/genetics
MH  - Telencephalon/*metabolism
PMC - PMC6349034
MID - NIHMS979296
OTO - NOTNLM
OT  - Astrocyte
OT  - Cortex
OT  - GluN2C
OT  - GluN2D
OT  - Hippocampus
OT  - L-glutamate
OT  - N-methyl-D-aspartate receptor
OT  - mRNA
EDAT- 2018/04/14 06:00
MHDA- 2019/03/15 06:00
PMCR- 2020/01/01
CRDT- 2018/04/14 06:00
PHST- 2018/02/13 00:00 [received]
PHST- 2018/04/06 00:00 [accepted]
PHST- 2018/03/30 00:00 [revised]
PHST- 2018/04/14 06:00 [pubmed]
PHST- 2019/03/15 06:00 [medline]
PHST- 2018/04/14 06:00 [entrez]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.1007/s11064-018-2526-7 [pii]
AID - 10.1007/s11064-018-2526-7 [doi]
PST - ppublish
SO  - Neurochem Res. 2019 Jan;44(1):61-77. doi: 10.1007/s11064-018-2526-7. Epub 2018 
      Apr 12.