PMID- 29651947
OWN - NLM
STAT- MEDLINE
DCOM- 20190409
LR  - 20190409
IS  - 1875-5631 (Electronic)
IS  - 1566-5232 (Linking)
VI  - 18
IP  - 1
DP  - 2018
TI  - Transplantation of BDNF Gene Recombinant Mesenchymal Stem Cells and Adhesive 
      Peptide-modified Hydrogel Scaffold for Spinal Cord Repair.
PG  - 29-39
LID - 10.2174/1566523218666180413150023 [doi]
AB  - INTRODUCTION: Mesenchymal Stem Cells (MSCs) are promising candidates for nerve 
      tissue engineering. Brain Derived Neurotrophic Factor (BDNF) secreted by MSCs can 
      function to increase neural differentiation and relieve inflammation response. 
      Gene transfection technology is an efficient strategy to increase the secretion 
      levels of cytokines and enhance cellular functions. However, transfection and in 
      vivo gene expression of environmentally sensitive stem cells have been one of the 
      most challenging subjects due to the requirement in both safety and transfection 
      efficiency. In this study, gene transfection technology was applied to prepare 
      BDNF gene recombinant MSCs based on our previously reported liposomal vector 
      ScreenFect(R) A. To improve cellular survival and gene expression after in situ 
      implantation of MSCs, an adhesive peptide modified hydrogel scaffold was 
      constructed using hyaluronic acid. The scaffold was optimized and modified with 
      an adhesive peptide PPFLMLLKGSTR. The transfected MSCs exhibited improved 
      cellular survival and sustained gene expression in the three-Dimentional (3D) 
      scaffold in vitro. Compared to untransfected MSCs, gene recombinant MSCs 
      effectively improved spinal tissue integrity, inhibited glial scar formation and 
      alleviated inflammatory response. These effects were found discounted when cells 
      were implanted without the scaffold. CONCLUSION: The study developed a promising 
      implantation system for therapy of severe spinal cord injury and provided the 
      first understanding of Screenfect(R) A about its functions on stem cell therapy for 
      nerve tissue repair as well as three-dimentional gene expression.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.org.
FAU - Li, Li-Ming
AU  - Li LM
AD  - Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang 
      University, Hangzhou, Zhejiang, China.
AD  - Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, 
      Zhejiang University, Hangzhou, Zhejiang, China.
FAU - Huang, Ling-Ling
AU  - Huang LL
AD  - Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang 
      University, Hangzhou, Zhejiang, China.
FAU - Jiang, Xin-Chi
AU  - Jiang XC
AD  - Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang 
      University, Hangzhou, Zhejiang, China.
FAU - Chen, Jia-Chen
AU  - Chen JC
AD  - Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang 
      University, Hangzhou, Zhejiang, China.
FAU - OuYang, Hong-Wei
AU  - OuYang HW
AD  - Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, 
      Zhejiang University, Hangzhou, Zhejiang, China.
FAU - Gao, Jian-Qing
AU  - Gao JQ
AD  - Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang 
      University, Hangzhou, Zhejiang, China.
AD  - Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, 
      Zhejiang University, Hangzhou, Zhejiang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United Arab Emirates
TA  - Curr Gene Ther
JT  - Current gene therapy
JID - 101125446
RN  - 0 (Adhesives)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Hydrogels)
RN  - 0 (Peptides)
SB  - IM
MH  - Adhesives/chemistry
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Female
MH  - Genetic Therapy/*methods
MH  - Humans
MH  - Hydrogels/chemistry
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Peptides/chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/genetics/*therapy
MH  - Tissue Scaffolds/chemistry
MH  - Transfection
OTO - NOTNLM
OT  - Adhesive peptide
OT  - Brain derived neurotrophic factor
OT  - Gene transfection
OT  - Hydrogel scaffold
OT  - Mesenchymal stem cells
OT  - Spinal cord injury.
EDAT- 2018/04/14 06:00
MHDA- 2019/04/10 06:00
CRDT- 2018/04/14 06:00
PHST- 2017/07/07 00:00 [received]
PHST- 2017/11/24 00:00 [revised]
PHST- 2017/11/30 00:00 [accepted]
PHST- 2018/04/14 06:00 [pubmed]
PHST- 2019/04/10 06:00 [medline]
PHST- 2018/04/14 06:00 [entrez]
AID - CGT-EPUB-89715 [pii]
AID - 10.2174/1566523218666180413150023 [doi]
PST - ppublish
SO  - Curr Gene Ther. 2018;18(1):29-39. doi: 10.2174/1566523218666180413150023.