PMID- 29654626
OWN - NLM
STAT- MEDLINE
DCOM- 20181016
LR  - 20181202
IS  - 1365-2885 (Electronic)
IS  - 0140-7783 (Linking)
VI  - 41
IP  - 4
DP  - 2018 Aug
TI  - Effects of enrofloxacin on antioxidant system, microsomal enzymatic activity, and 
      proteomics in porcine liver.
PG  - 562-571
LID - 10.1111/jvp.12493 [doi]
AB  - Enrofloxacin (EF) is a widely used fluoroquinolone, usually regarded as a safe 
      and effective treatment for bacterial infections. Adverse effects of EF have 
      previously been demonstrated in some species, but so far there have been no 
      studies looking specifically at the impact of EF on pigs. In this study, three 
      different doses of EF (5, 25 and 125 mg kg bw(-1) ) were administrated to Bama 
      pigs. The results showed that lipid peroxidation of pig liver tissue occurred 
      with all EF doses. The 125 mg kg dose of EF induced catalase (CAT) and 
      glutathione peroxidase (GSH-px) and increased CYP450 content in pig liver 
      microsomes. The activity of microsomal NADPH-cytochrome C reductase (NCCR) was 
      elevated at both the 25 and 125 mg kg doses of EF. Microsomal erythromycin 
      N-demethylase (ERND) and aminopyrin N-demethylase (AND) were inhibited by high 
      doses of EF, while aniline-4-hydroxylase (AH) was unaffected. None of the EF 
      treatments affected superoxide dismutase (SOD) or cytochrome b5 content. 
      Antioxidases and microsomal enzymes may work together to resist the adverse 
      effects of EF. Proteomic analysis revealed increased protein expression of 
      carboxylesterase (CES) and alpha-enolase (ENO1) in microsomes as a stress 
      response to EF. These results provide new information about the adverse effect of 
      fluoroquinolones and help guide their usage more effectively in the clinic or 
      animal breeding.
CI  - (c) 2018 John Wiley & Sons Ltd.
FAU - Li, Y
AU  - Li Y
AUID- ORCID: 0000-0003-2629-7941
AD  - School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 
      China.
FAU - Mou, Y
AU  - Mou Y
AD  - School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 
      China.
FAU - Thunders, M
AU  - Thunders M
AD  - College of Health, Massey University, Wellington, New Zealand.
FAU - Wu, Y
AU  - Wu Y
AD  - School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 
      China.
FAU - Ai, X
AU  - Ai X
AD  - School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 
      China.
FAU - Zhou, X
AU  - Zhou X
AD  - School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 
      China.
FAU - Qiu, J
AU  - Qiu J
AD  - School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20180414
PL  - England
TA  - J Vet Pharmacol Ther
JT  - Journal of veterinary pharmacology and therapeutics
JID - 7910920
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Fluoroquinolones)
RN  - 3DX3XEK1BN (Enrofloxacin)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP3A)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.5.3.- (Aminopyrine N-Demethylase)
RN  - EC 1.6.2.4 (NADPH-Ferrihemoprotein Reductase)
SB  - IM
MH  - Aminopyrine N-Demethylase/metabolism
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Antioxidants/*metabolism
MH  - Catalase/metabolism
MH  - Catalepsy
MH  - Cytochrome P-450 CYP3A/metabolism
MH  - Cytochrome P-450 Enzyme System/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enrofloxacin
MH  - Female
MH  - Fluoroquinolones/*pharmacology
MH  - Glutathione Peroxidase/metabolism
MH  - Lipid Peroxidation/drug effects
MH  - Liver/*drug effects/enzymology/metabolism
MH  - Male
MH  - Microsomes, Liver/*drug effects/enzymology/metabolism
MH  - NADPH-Ferrihemoprotein Reductase/metabolism
MH  - Proteomics
MH  - Superoxide Dismutase/metabolism
MH  - Swine
OTO - NOTNLM
OT  - CYP450
OT  - enrofloxacin
OT  - microsome
OT  - pig
OT  - proteomics
EDAT- 2018/04/15 06:00
MHDA- 2018/10/17 06:00
CRDT- 2018/04/15 06:00
PHST- 2017/10/13 00:00 [received]
PHST- 2018/01/06 00:00 [accepted]
PHST- 2018/04/15 06:00 [pubmed]
PHST- 2018/10/17 06:00 [medline]
PHST- 2018/04/15 06:00 [entrez]
AID - 10.1111/jvp.12493 [doi]
PST - ppublish
SO  - J Vet Pharmacol Ther. 2018 Aug;41(4):562-571. doi: 10.1111/jvp.12493. Epub 2018 
      Apr 14.