PMID- 29654659
OWN - NLM
STAT- MEDLINE
DCOM- 20191213
LR  - 20210109
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 22
IP  - 7
DP  - 2018 Jul
TI  - Human umbilical cord mesenchymal stem cells facilitate the up-regulation of 
      miR-153-3p, whereby attenuating MGO-induced peritoneal fibrosis in rats.
PG  - 3452-3463
LID - 10.1111/jcmm.13622 [doi]
AB  - MiRNAs contribute greatly to epithelial to mesenchymal transition (EMT) of 
      peritoneal mesothelial cells (PMCs), which is a crucial step in peritoneal 
      fibrosis (PF). In this study, we tried to profile whether miRNA expression 
      differences exist after human umbilical cord mesenchymal stem cells (hUCMSCs) 
      treatment in PF rats and investigate the possible role of miR-153-3p involved in 
      anti-EMT process. We randomly assigned 34 rats into three groups: control group 
      (Group Control), MGO-induced PF rats (Group MGO) and hUCMSCs-treated rats (Group 
      MGO + hUCMSCs). MiRNA microarrays and real-time PCR analyses were conducted in 
      three groups. alpha-SMA, Snail1 and E-cadherin expression were detected by Western 
      blot. Luciferase reporter assays were used to detect the effects of miR-153-3p 
      overexpression on Snai1 in rat peritoneal mesothelial cells (RPMCs). We 
      identified differentially expressed miRNAs related to EMT, in which miR-153-3p 
      demonstrated the greatest increase in Group MGO + hUCMSCs. Transient 
      cotransfection of miR-153-3p mimics with luciferase expression plasmids resulted 
      in a significant repression of Snai1 3'-untranslated region luciferase activity 
      in RPMCs. These studies suggest that miR-153-3p is a critical molecule in 
      anti-EMT effects of hUCMSCs in MGO-induced PF rats. MiR-153-3p might exert its 
      beneficial effect through directly targeting Snai1.
CI  - (c) 2018 The Authors. Journal of Cellular and Molecular Medicine published by John 
      Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
FAU - Li, Dong
AU  - Li D
AD  - Department of Nephrology, General Hospital of Tianjin Medical University, 
      Tianjin, China.
FAU - Lu, Zhenyu
AU  - Lu Z
AD  - Tianjin Precell Biotechnology Co., Ltd., Huayuan Industrial District, Tianjin, 
      China.
FAU - Li, Xiyuan
AU  - Li X
AD  - Precision Medical Center, General Hospital of Tianjin Medical University, 
      Tianjin, China.
FAU - Xu, Zhongwei
AU  - Xu Z
AD  - Central Laboratory, Logistics University of the Chinese People's Armed Police 
      Force, Tianjin, China.
FAU - Jiang, Jianqing
AU  - Jiang J
AD  - Department of Nephrology, General Hospital of Tianjin Medical University, 
      Tianjin, China.
FAU - Zheng, Zhenfeng
AU  - Zheng Z
AD  - Department of Nephrology, General Hospital of Tianjin Medical University, 
      Tianjin, China.
FAU - Jia, Junya
AU  - Jia J
AD  - Department of Nephrology, General Hospital of Tianjin Medical University, 
      Tianjin, China.
FAU - Lin, Shan
AU  - Lin S
AUID- ORCID: 0000-0002-7679-3145
AD  - Department of Nephrology, General Hospital of Tianjin Medical University, 
      Tianjin, China.
FAU - Yan, Tiekun
AU  - Yan T
AD  - Department of Nephrology, General Hospital of Tianjin Medical University, 
      Tianjin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180414
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (MIRN153 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Snail Family Transcription Factors)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (snai1 protein, rat)
RN  - 722KLD7415 (Pyruvaldehyde)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/pharmacology
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - Gene Expression Regulation
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*metabolism
MH  - MicroRNAs/*genetics
MH  - Peritoneal Fibrosis/chemically induced/*genetics/pathology/*therapy
MH  - Pyruvaldehyde
MH  - Rats, Wistar
MH  - Snail Family Transcription Factors/genetics
MH  - Transforming Growth Factor beta1/pharmacology
MH  - Umbilical Cord/cytology
MH  - Up-Regulation
PMC - PMC6010808
OTO - NOTNLM
OT  - epithelial to mesenchymal transition
OT  - human umbilical cord mesenchymal stem cells
OT  - microRNA
OT  - peritoneal fibrosis
OT  - peritoneal mesothelial cells
EDAT- 2018/04/15 06:00
MHDA- 2019/12/18 06:00
PMCR- 2018/07/01
CRDT- 2018/04/15 06:00
PHST- 2018/01/16 00:00 [received]
PHST- 2018/03/03 00:00 [accepted]
PHST- 2018/04/15 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2018/04/15 06:00 [entrez]
PHST- 2018/07/01 00:00 [pmc-release]
AID - JCMM13622 [pii]
AID - 10.1111/jcmm.13622 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2018 Jul;22(7):3452-3463. doi: 10.1111/jcmm.13622. Epub 2018 Apr 
      14.