PMID- 29657680
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220317
IS  - 1999-768X (Print)
IS  - 2070-5204 (Electronic)
IS  - 1999-768X (Linking)
VI  - 33
IP  - 2
DP  - 2018 Mar
TI  - Evaluation of the Role of -137G/C Single Nucleotide Polymorphism (rs187238) and 
      Gene Expression Levels of the IL-18 in Patients with Coronary Artery Disease.
PG  - 118-125
LID - 10.5001/omj.2018.23 [doi]
AB  - OBJECTIVES: Interleukin-18 (IL-18) is a proinflammatory and proatherogenic 
      cytokine, and its genetic variations may contribute to the development of 
      coronary artery disease (CAD). We sought to investigate the role of -137G/C 
      polymorphism and gene expression levels of IL-18 in patients with CAD. METHODS: 
      The study population included 100 patients with angiographically proven CAD and 
      100 matched controls. Total RNA and DNA were extracted from leukocytes using 
      appropriate kits. The genotype of -137G/C polymorphism and gene expression level 
      of IL-18 was determined using allele-specific polymerase chain reaction (PCR) and 
      real-time (RT)-PCR assay, respectively. RESULTS: The genotypic and allelic 
      distribution of IL-18 -137G/C polymorphism was not significantly different 
      between the two groups (p > 0.050). Moreover, the -137G/C polymorphism did not 
      increase the risk of CAD in dominant and recessive genetic models (p > 0.050). 
      However, subgroup analysis of CAD patients revealed that the IL-18 -137G/C 
      polymorphism was significantly associated with increased risk of CAD in 
      hypertensive patients (odds ratio (OR) = 7.51; 95% confidence interval (CI): 
      1.24-25.17; p = 0.019) and smokers (OR = 4.90; 95% CI: 1.21-19.70; p = 0.031) but 
      not in the diabetic subpopulation (p = 0.261). The genotype distribution of IL-18 
      -137G/C genetic polymorphism was significantly different among patients with one, 
      two, and three stenotic vessels (p < 0.050). The gene expression level of IL-18 
      was significantly higher in the CAD group than the control group (p < 0.001). 
      Moreover, the carriers of CC genotype had significantly lower gene expression 
      levels of IL-18 than carriers of GG genotype (p < 0.050). CONCLUSIONS: The 
      -137G/C polymorphism of IL-18 may be associated with the CAD risk in hypertensive 
      and smoker subgroup of CAD patients. The -137G/C polymorphism seems to play an 
      important role in determining the severity of CAD. Increased IL-18 gene 
      expression level is a significant risk factor for the development of CAD. The CC 
      genotype of -137G/C polymorphism is associated with lower IL-18 gene expression 
      levels.
FAU - Hoseini, Fatemeh
AU  - Hoseini F
AD  - Department of Genetic, Faculty of Basic Sciences, Islamic Azad University, Zanjan 
      Branch, Zanjan, Iran.
FAU - Mahmazi, Sanaz
AU  - Mahmazi S
AD  - Department of Genetic, Faculty of Basic Sciences, Islamic Azad University, Zanjan 
      Branch, Zanjan, Iran.
FAU - Mahmoodi, Khalil
AU  - Mahmoodi K
AD  - Department of Cardiology, School of Medicine, Zanjan University of Medical 
      Sciences, Zanjan, Iran.
FAU - Jafari, Gholam Ali
AU  - Jafari GA
AD  - Department of Microbiology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Soltanpour, Mohammad Soleiman
AU  - Soltanpour MS
AD  - Department of Medical Laboratory Sciences, School of Paramedical Sciences, Zanjan 
      University of Medical Sciences, Zanjan, Iran.
LA  - eng
PT  - Journal Article
PL  - Oman
TA  - Oman Med J
JT  - Oman medical journal
JID - 101526350
PMC - PMC5889842
OTO - NOTNLM
OT  - Coronary Artery Disease
OT  - Gene Expression
OT  - Interleukin-18
OT  - Polymerase Chain Reaction
OT  - Polymorphism, Restriction Fragment Length
EDAT- 2018/04/17 06:00
MHDA- 2018/04/17 06:01
PMCR- 2018/03/01
CRDT- 2018/04/17 06:00
PHST- 2018/04/17 06:00 [entrez]
PHST- 2018/04/17 06:00 [pubmed]
PHST- 2018/04/17 06:01 [medline]
PHST- 2018/03/01 00:00 [pmc-release]
AID - OMJ-D-17-00117 [pii]
AID - 10.5001/omj.2018.23 [doi]
PST - ppublish
SO  - Oman Med J. 2018 Mar;33(2):118-125. doi: 10.5001/omj.2018.23.