PMID- 29659673
OWN - NLM
STAT- MEDLINE
DCOM- 20190506
LR  - 20220408
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 149
IP  - 6
DP  - 2018 Apr 25
TI  - Evaluation of the Utility of Bone Marrow Morphology and Ancillary Studies in 
      Pediatric Patients Under Surveillance for Myelodysplastic Syndrome.
PG  - 499-513
LID - 10.1093/ajcp/aqy007 [doi]
AB  - OBJECTIVES: To evaluate the utility of flow cytometry, karyotype, and a 
      fluorescence in situ hybridization (FISH) panel in screening children for 
      myelodysplastic syndrome (MDS). METHODS: Bone marrow morphology, flow cytometry, 
      karyotype, and FISH reports from 595 bone marrow specimens (246 patients) were 
      analyzed. RESULTS: By morphology, 8.7% of cases demonstrated at least unilineage 
      dysplasia and/or increased blasts. Flow cytometry identified definitive 
      abnormalities in 2.8% of cases, all of which had abnormal morphology. Of the 42 
      cases (7.2%) with acquired karyotypic abnormalities, 26 had no morphologic 
      dysplasia. With a 98.2% concordance between karyotype and MDS FISH, FISH only 
      identified two additional cases, both with low-level (<4%) abnormalities. 
      Peripheral blood count evaluation only identified the absence of thrombocytopenia 
      to correlate with an absence of abnormal ancillary tests. CONCLUSIONS: The 
      combination of morphologic evaluation and karyotype with judicious use of flow 
      cytometry and MDS FISH is sufficient to detect abnormalities for these 
      indications.
FAU - Chisholm, Karen M
AU  - Chisholm KM
AD  - Department of Laboratories, Seattle, WA.
AD  - Department of Laboratory Medicine, University of Washington, Seattle.
FAU - Xu, Min
AU  - Xu M
AD  - Department of Laboratories, Seattle, WA.
AD  - Department of Laboratory Medicine, University of Washington, Seattle.
FAU - Davis, Billy
AU  - Davis B
AD  - Department of Laboratories, Seattle, WA.
FAU - Ogi, Amy
AU  - Ogi A
AD  - Department of Laboratories, Seattle, WA.
FAU - Pacheco, M Cristina
AU  - Pacheco MC
AD  - Department of Laboratories, Seattle, WA.
AD  - Department of Pathology, University of Washington, Seattle.
FAU - Geddis, Amy E
AU  - Geddis AE
AD  - Laboratories Cancer and Blood Disorders Center, Seattle Children's Hospital, 
      Seattle, WA.
AD  - Division of Hematology and Oncology, Department of Pediatrics, University of 
      Washington, Seattle.
FAU - Tsuchiya, Karen D
AU  - Tsuchiya KD
AD  - Department of Laboratories, Seattle, WA.
AD  - Department of Laboratory Medicine, University of Washington, Seattle.
FAU - Rutledge, Joe C
AU  - Rutledge JC
AD  - Department of Laboratories, Seattle, WA.
AD  - Department of Laboratory Medicine, University of Washington, Seattle.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - Ancillary Services, Hospital
MH  - Blood Cell Count/*methods
MH  - Bone Marrow/pathology
MH  - Child
MH  - *Chromosome Aberrations
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Male
MH  - Myelodysplastic Syndromes/*diagnosis/genetics/pathology
MH  - Retrospective Studies
EDAT- 2018/04/17 06:00
MHDA- 2019/05/07 06:00
CRDT- 2018/04/17 06:00
PHST- 2018/04/17 06:00 [pubmed]
PHST- 2019/05/07 06:00 [medline]
PHST- 2018/04/17 06:00 [entrez]
AID - 4962417 [pii]
AID - 10.1093/ajcp/aqy007 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2018 Apr 25;149(6):499-513. doi: 10.1093/ajcp/aqy007.