PMID- 29659875
OWN - NLM
STAT- MEDLINE
DCOM- 20181218
LR  - 20191210
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 42
IP  - 7
DP  - 2018 Sep 1
TI  - Sensitive and simultaneous determination of nine anticoagulant rodenticides in 
      human blood by UPLC-MS-MS with phospholipid removal pretreatment.
PG  - 459-466
LID - 10.1093/jat/bky024 [doi]
AB  - A sensitive and rapid method for the simultaneous determination of nine 
      anticoagulant rodenticides (ARs) in human blood is reported herein. The method 
      involves phospholipid removal pretreatment for reduced matrix effect (ME) and 
      detection with ultra-performance liquid chromatography coupled with tandem mass 
      spectrometry. Satisfactory recoveries were achieved ranging from 80.6% to 113.1% 
      for the nine analytes, with the intra-day relative standard deviations (RSDs) in 
      the range of 3.4-7.9% and inter-day RSDs in the range of 4.1-8.3%, indicating 
      good precision. Linear relationships with correlation coefficients above 0.998 (n 
      = 6) were found in the range of 1-2,000 ng/mL. High sensitivity was achieved with 
      limits of detection ranging from 0.02 to 0.3. The application of phospholipid 
      removal step significantly optimized the ME, and the reduction of ME ranged from 
      6.1% to 15.5%. This method was successfully applied to the determination of ARs 
      for blood samples from real forensic cases. These results prove that this method 
      is reliable for rapid forensic and clinical diagnosis. The removal capabilities 
      for five representative phospholipids that are abundant in blood were evaluated 
      individually with Phree phospholipid removal plates. While significant 
      capabilities for phospholipid removal were confirmed, the results showed that the 
      removal capability for certain phospholipid could be improved.
FAU - Guo, Hao
AU  - Guo H
AD  - Chongqing Institute of Forensic Science, Chongqing, China.
FAU - Wang, Junwei
AU  - Wang J
AD  - Chongqing Institute of Forensic Science, Chongqing, China.
FAU - Wu, Yuhong
AU  - Wu Y
AD  - Department of Forensic Science and Technology, Chongqing Police College, 
      Chongqing, China.
FAU - Liu, Wenwen
AU  - Liu W
AD  - Chongqing Institute of Forensic Science, Chongqing, China.
FAU - Bu, Juan
AU  - Bu J
AD  - Department of Forensic Science and Technology, Chongqing Police College, 
      Chongqing, China.
FAU - Zhao, Qingbiao
AU  - Zhao Q
AD  - Key Laboratory of Polar Materials and Devices, Department of Electronic 
      Engineering, Ministry of Education, East China Normal University, Shanghai, 
      China.
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Anticoagulants)
RN  - 0 (Phospholipids)
RN  - 0 (Rodenticides)
SB  - IM
MH  - Anticoagulants/*blood/poisoning
MH  - Blood Coagulation/drug effects
MH  - Child, Preschool
MH  - Chromatography, Liquid/*methods
MH  - Epistaxis/chemically induced
MH  - Female
MH  - Forensic Toxicology/*methods
MH  - Gingival Hemorrhage/chemically induced
MH  - Humans
MH  - Phospholipids/*blood
MH  - Poisoning/blood/diagnosis
MH  - Reproducibility of Results
MH  - Rodenticides/*blood/poisoning
MH  - *Tandem Mass Spectrometry
EDAT- 2018/04/17 06:00
MHDA- 2018/12/19 06:00
CRDT- 2018/04/17 06:00
PHST- 2017/12/12 00:00 [received]
PHST- 2018/03/18 00:00 [accepted]
PHST- 2018/04/17 06:00 [pubmed]
PHST- 2018/12/19 06:00 [medline]
PHST- 2018/04/17 06:00 [entrez]
AID - 4962185 [pii]
AID - 10.1093/jat/bky024 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2018 Sep 1;42(7):459-466. doi: 10.1093/jat/bky024.