PMID- 29660438
OWN - NLM
STAT- MEDLINE
DCOM- 20190108
LR  - 20220115
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Linking)
VI  - 352
DP  - 2018 Aug 1
TI  - Prenatal nicotine exposure intergenerationally programs imperfect articular 
      cartilage via histone deacetylation through maternal lineage.
PG  - 107-118
LID - S0041-008X(18)30104-2 [pii]
LID - 10.1016/j.taap.2018.03.018 [doi]
AB  - Accumulating evidence has shown that the impact of prenatal environmental factors 
      on the organs of the offspring could last until the adulthood. Here, we aimed to 
      investigate these effects and the potential mechanism of prenatal nicotine 
      exposure (PNE) on the female adult cartilage of the first generation (PNE-F1) and 
      the second generation (PNE-F2). Pregnant Wistar rats were injected with 
      2.0 mg/kg.d nicotine from gestational day (GD) 9 to 20. Then their F1 generation 
      at GD20 and postnatal week (PW) 12, and F2 generation at PW12 were harvested. The 
      expression of extracellular matrix (ECM) and transforming growth factor beta (TGFbeta) 
      signaling genes were analyzed by real-time quantitative PCR, and the histone 
      acetylation was examined by chromatin immunoprecipitation assay. The results 
      showed that PNE reduced the ECM and TGFbeta signaling gene expressions in both 
      PNE-F1 and PNE-F2 female adult articular cartilage. In the F1 generation, PNE 
      inhibited the acetylation at H3K9 of TGFbeta, TGFbeta receptor 1 (TGFbetaR1), SRY-type 
      high mobility group box 9 (SOX9), a1 chain of type II collagen (COL2A1) and 
      aggrecan (ACAN) gene promoters at both GD20 and PW12. In PNE-F2 at PW12, the 
      obvious deacetylation at H3K9 of the TGFbetaR1 and COL2A1 promoters still existed. 
      Moreover, in rat fetal chondrocytes, corticosterone rather than nicotine directly 
      induced the hypoacetylation of H3K9 of TGFbetaR1 and COL2A1 genes, which might be 
      the main cause of imperfect cartilage for PNE-F2. This study may be helpful to 
      elucidate the developmental variability of articular cartilage quality and useful 
      for the early prevention of articular damage.
CI  - Copyright (c) 2018. Published by Elsevier Inc.
FAU - Xie, Zhe
AU  - Xie Z
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Hubei Provincial Key Laboratory of Developmentally Originated 
      Diseases, 185 Donghu Road, Wuchang District, Wuhan 430071, China.
FAU - Zhao, Zhe
AU  - Zhao Z
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China.
FAU - Yang, Xu
AU  - Yang X
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Hubei Provincial Key Laboratory of Developmentally Originated 
      Diseases, 185 Donghu Road, Wuchang District, Wuhan 430071, China.
FAU - Pei, Linguo
AU  - Pei L
AD  - Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 
      430071, China.
FAU - Luo, Hanwen
AU  - Luo H
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China.
FAU - Ni, Qubo
AU  - Ni Q
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Hubei Provincial Key Laboratory of Developmentally Originated 
      Diseases, 185 Donghu Road, Wuchang District, Wuhan 430071, China.
FAU - Li, Bin
AU  - Li B
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China.
FAU - Qi, Yongjian
AU  - Qi Y
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Hubei Provincial Key Laboratory of Developmentally Originated 
      Diseases, 185 Donghu Road, Wuchang District, Wuhan 430071, China.
FAU - Tie, Kai
AU  - Tie K
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Hubei Provincial Key Laboratory of Developmentally Originated 
      Diseases, 185 Donghu Road, Wuchang District, Wuhan 430071, China.
FAU - Magdalou, Jacques
AU  - Magdalou J
AD  - UMR 7561CNRS, Universite de Lorraine, Faculte de Medicine, Vandoeuvre-les-Nancy, 
      France.
FAU - Chen, Liaobin
AU  - Chen L
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Hubei Provincial Key Laboratory of Developmentally Originated 
      Diseases, 185 Donghu Road, Wuchang District, Wuhan 430071, China. Electronic 
      address: lbchen@whu.edu.cn.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Department of Pharmacology, Basic Medical School of Wuhan 
      University, Wuhan 430071, China; Hubei Provincial Key Laboratory of 
      Developmentally Originated Diseases, 185 Donghu Road, Wuchang District, Wuhan 
      430071, China. Electronic address: wanghui19@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180413
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Aggrecans)
RN  - 0 (COL2A1 protein, rat)
RN  - 0 (Collagen Type II)
RN  - 0 (Histones)
RN  - 0 (Nicotinic Agonists)
RN  - 6M3C89ZY6R (Nicotine)
RN  - EC 2.7.11.30 (Receptor, Transforming Growth Factor-beta Type I)
RN  - EC 2.7.11.30 (Tgfbr1 protein, rat)
SB  - IM
EIN - Toxicol Appl Pharmacol. 2022 Feb 15;437:115865. PMID: 35032483
MH  - Acetylation
MH  - Age Factors
MH  - Aggrecans/genetics/metabolism
MH  - Animals
MH  - Cartilage, Articular/*drug effects/metabolism/pathology
MH  - Cells, Cultured
MH  - Chondrocytes/drug effects/metabolism/pathology
MH  - Chondrogenesis/*drug effects/genetics
MH  - Collagen Type II/genetics/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Gestational Age
MH  - Histones/*metabolism
MH  - Male
MH  - Nicotine/administration & dosage/*toxicity
MH  - Nicotinic Agonists/administration & dosage/*toxicity
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats, Wistar
MH  - Receptor, Transforming Growth Factor-beta Type I/genetics/metabolism
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Articular cartilage
OT  - Histone deacetylation modification
OT  - Intergenerational transmission
OT  - Nicotine prenatal exposure
OT  - Transforming growth factor beta
EDAT- 2018/04/17 06:00
MHDA- 2019/01/09 06:00
CRDT- 2018/04/17 06:00
PHST- 2017/11/11 00:00 [received]
PHST- 2018/02/15 00:00 [revised]
PHST- 2018/03/14 00:00 [accepted]
PHST- 2018/04/17 06:00 [pubmed]
PHST- 2019/01/09 06:00 [medline]
PHST- 2018/04/17 06:00 [entrez]
AID - S0041-008X(18)30104-2 [pii]
AID - 10.1016/j.taap.2018.03.018 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2018 Aug 1;352:107-118. doi: 10.1016/j.taap.2018.03.018. 
      Epub 2018 Apr 13.