PMID- 29662857
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220317
IS  - 2287-979X (Print)
IS  - 2288-0011 (Electronic)
IS  - 2287-979X (Linking)
VI  - 53
IP  - 1
DP  - 2018 Mar
TI  - Cryohemolysis, erythrocyte osmotic fragility, and supplementary hematimetric 
      indices in the diagnosis of hereditary spherocytosis.
PG  - 10-17
LID - 10.5045/br.2018.53.1.10 [doi]
AB  - BACKGROUND: Hereditary spherocytosis (HS) is a chronic hemolytic anemia 
      characterized by microspherocytes in the peripheral blood and increased 
      erythrocyte osmotic fragility (EOF). This study evaluated the cryohemolysis test 
      (CHT); initial hemolysis (IH); immediate and incubated hemolysis percentage in 
      5.5 g/L NaCl (H5.5); mean corpuscular hemoglobin concentration (MCHC); red blood 
      cell distribution width (RDW); and Hb/MCHC, Hb/RDW, and MCHC/RDW ratios for the 
      diagnosis of HS. METHODS: Data from 13 patients with HS were evaluated at the 
      Instituto de Bioquimica Aplicada and compared with data from 14 unaffected 
      individuals and 11 patients with anemia due to another etiology. Total blood and 
      reticulocyte counts, CHT, and immediate and incubated EOF were performed in all 
      subjects; sensitivity, specificity, efficiency, and Youden index (YI) were 
      calculated. RESULTS: Eight patients with HS had MCHC >/=345 g/L, 10 had RDW >/=14.5%, 
      12 had IH >5.0 g/L, 11 had immediate H5.5 >/=5%, and 13 had incubated H5.5 >/=50% 
      (the cut-off value to consider HS). The efficiency and YI were: immediate H5.5 
      (0.94-0.85), incubated H5.5 (0.89-0.82), IH (0.89-0.78), MCHC (0.87-0.62), CHT 
      (0.84-0.54), and Hb/MCHC (0.71-0.56), respectively. The calculated ratios could 
      distinguish subjects with HS from unaffected individuals (P<0.05), but not those 
      with anemia of another etiology (P>0.05). CONCLUSION: Although the CHT and 
      supplementary hematimetric indexes were useful to differentiate individuals with 
      SH from healthy controls, they cannot distinguish from anemias of other etiology. 
      CHT and MCHC, in addition to EOF, are recommended for diagnosing HS patients 
      because of their low cost and efficiency.
FAU - Emilse, Ledesma Achem Miryam
AU  - Emilse LAM
AD  - Instituto de Bioquimica Aplicada, Facultad de Bioquimica, Quimica y Farmacia, 
      Universidad Nacional de Tucuman, Tucuman, Argentina.
FAU - Cecilia, Haro
AU  - Cecilia H
AD  - Instituto de Bioquimica Aplicada, Facultad de Bioquimica, Quimica y Farmacia, 
      Universidad Nacional de Tucuman, Tucuman, Argentina.
FAU - Maria, Teran Magdalena
AU  - Maria TM
AD  - Instituto de Bioquimica Aplicada, Facultad de Bioquimica, Quimica y Farmacia, 
      Universidad Nacional de Tucuman, Tucuman, Argentina.
FAU - Eugenia, Monaco Maria
AU  - Eugenia MM
AD  - Instituto de Biologia, Facultad de Bioquimica, Quimica y Farmacia, Universidad 
      Nacional de Tucuman, Tucuman, Argentina.
FAU - Alicia, Isse Blanca
AU  - Alicia IB
AD  - Instituto de Bioquimica Aplicada, Facultad de Bioquimica, Quimica y Farmacia, 
      Universidad Nacional de Tucuman, Tucuman, Argentina.
FAU - Lazarte, Sandra Stella
AU  - Lazarte SS
AD  - Instituto de Bioquimica Aplicada, Facultad de Bioquimica, Quimica y Farmacia, 
      Universidad Nacional de Tucuman, Tucuman, Argentina.
LA  - eng
PT  - Journal Article
DEP - 20180327
PL  - Switzerland
TA  - Blood Res
JT  - Blood research
JID - 101605247
PMC - PMC5898988
OTO - NOTNLM
OT  - Cryohemolysis
OT  - Erythrocyte osmotic fragility
OT  - Hematimetric indexes
OT  - Hereditary spherocytosis
COIS- Authors' Disclosures of Potential Conflicts of Interest: No potential conflicts 
      of interest relevant to this article were reported.
EDAT- 2018/04/18 06:00
MHDA- 2018/04/18 06:01
PMCR- 2018/03/01
CRDT- 2018/04/18 06:00
PHST- 2017/06/13 00:00 [received]
PHST- 2017/07/20 00:00 [revised]
PHST- 2017/08/30 00:00 [accepted]
PHST- 2018/04/18 06:00 [entrez]
PHST- 2018/04/18 06:00 [pubmed]
PHST- 2018/04/18 06:01 [medline]
PHST- 2018/03/01 00:00 [pmc-release]
AID - 10.5045/br.2018.53.1.10 [doi]
PST - ppublish
SO  - Blood Res. 2018 Mar;53(1):10-17. doi: 10.5045/br.2018.53.1.10. Epub 2018 Mar 27.