PMID- 29662942
OWN - NLM
STAT- MEDLINE
DCOM- 20190111
LR  - 20220331
IS  - 2373-2822 (Electronic)
IS  - 2373-2822 (Linking)
VI  - 5
IP  - 2
DP  - 2018 Mar-Apr
TI  - Measuring and Validating the Levels of Brain-Derived Neurotrophic Factor in Human 
      Serum.
LID - 10.1523/ENEURO.0419-17.2018 [doi]
LID - ENEURO.0419-17.2018
AB  - Brain-derived neurotrophic factor (BDNF) secreted by neurons is a significant 
      component of synaptic plasticity. In humans, it is also present in blood 
      platelets where it accumulates following its biosynthesis in megakaryocytes. BDNF 
      levels are thus readily detectable in human serum and it has been abundantly 
      speculated that they may somehow serve as an indicator of brain function. 
      However, there is a great deal of uncertainty with regard to the range of BDNF 
      levels that can be considered normal, how stable these values are over time and 
      even whether BDNF levels can be reliably measured in serum. Using monoclonal 
      antibodies and a sandwich ELISA, this study reports on BDNF levels in the serum 
      of 259 volunteers with a mean value of 32.69 +/- 8.33 ng/ml (SD). The mean value 
      for the same cohort after 12 months was not significantly different (N = 226, 
      32.97 +/- 8.36 ng/ml SD, p = 0.19). Power analysis of these values indicates that 
      relatively large cohorts are necessary to identify significant differences, 
      requiring a group size of 60 to detect a 20% change. The levels determined by 
      ELISA could be validated by Western blot analyses using a BDNF monoclonal 
      antibody. While no association was observed with gender, a weak, positive 
      correlation was found with age. The overall conclusions are that BDNF levels can 
      be reliably measured in human serum, that these levels are quite stable over one 
      year, and that comparisons between two populations may only be meaningful if 
      cohorts of sufficient sizes are assembled.
FAU - Naegelin, Yvonne
AU  - Naegelin Y
AD  - School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom.
AD  - Department of Neurology, University Hospital Basel, Basel 4031, Switzerland.
FAU - Dingsdale, Hayley
AU  - Dingsdale H
AD  - School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom.
FAU - Sauberli, Katharina
AU  - Sauberli K
AD  - School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom.
FAU - Schadelin, Sabine
AU  - Schadelin S
AD  - Clinical Trial Unit, Department of Clinical Research, University Hospital Basel, 
      Basel 4031, Switzerland.
FAU - Kappos, Ludwig
AU  - Kappos L
AD  - Department of Neurology, University Hospital Basel, Basel 4031, Switzerland.
FAU - Barde, Yves-Alain
AU  - Barde YA
AUID- ORCID: 0000-0002-7627-461X
AD  - School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180322
PL  - United States
TA  - eNeuro
JT  - eNeuro
JID - 101647362
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antibodies, Monoclonal
MH  - Blood Chemical Analysis/*standards
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Reproducibility of Results
MH  - Young Adult
PMC - PMC5898630
OTO - NOTNLM
OT  - BDNF
OT  - ELISA
OT  - Western blotting
OT  - antibodies
OT  - biomarkers
OT  - platelets
EDAT- 2018/04/18 06:00
MHDA- 2019/01/12 06:00
PMCR- 2018/03/22
CRDT- 2018/04/18 06:00
PHST- 2017/12/05 00:00 [received]
PHST- 2018/02/23 00:00 [revised]
PHST- 2018/02/26 00:00 [accepted]
PHST- 2018/04/18 06:00 [entrez]
PHST- 2018/04/18 06:00 [pubmed]
PHST- 2019/01/12 06:00 [medline]
PHST- 2018/03/22 00:00 [pmc-release]
AID - eN-NWR-0419-17 [pii]
AID - 10.1523/ENEURO.0419-17.2018 [doi]
PST - epublish
SO  - eNeuro. 2018 Mar 22;5(2):ENEURO.0419-17.2018. doi: 10.1523/ENEURO.0419-17.2018. 
      eCollection 2018 Mar-Apr.