PMID- 29663394
OWN - NLM
STAT- MEDLINE
DCOM- 20181015
LR  - 20181015
IS  - 1097-0010 (Electronic)
IS  - 0022-5142 (Linking)
VI  - 98
IP  - 14
DP  - 2018 Nov
TI  - Polymorphism in ovine ANXA9 gene and the physio-chemical properties and the 
      fraction of protein in milk.
PG  - 5396-5400
LID - 10.1002/jsfa.9081 [doi]
AB  - BACKGROUND: Annexin A9 (ANXA9) is a specific fatty acid transport protein. The 
      ANXA9 gene is expressed in various tissues, including secretory tissue and the 
      mammary glands. The association between the three single nucleotide polymorphisms 
      (SNPs) of the ANXA9 gene and sheep's milk composition was assessed. RESULTS: 
      Genotype analysis was performed using the polymerase chain reaction-restriction 
      fragment length polymorphism (PCR-RFLP) method. The ANXA9 polymorphisms that were 
      studied had the following major allele frequencies (MAFs): SNP1: allele G 0,66; 
      SNP2: allele G 0,54; SNP3: allele C 0,57. The study found the most favorable 
      profile of protein fractions, namely increased kappa-casein fractions and a 
      decreased level of whey protein in sheep's milk for the SNP1 and SNP3 
      polymorphisms. Sheep with the SNP1 GA genotype had the highest (P < 0.05) content 
      of fat and dry matter in milk. AXNA9 gene polymorphism did not influence the 
      levels of protein, lactose, or urea in sheep's milk. CONCLUSION: The information 
      contained in this study may be useful for determining the impact of the ANXA9 
      gene on sheep's milk. The ANXA9 SNP1 and SNP3 polymorphism results could be 
      included in breeding programs to select sheep with the genotypes ensuring the 
      highest kappa-casein levels in milk. However, it is worth conducting further 
      research on ANXA9 and milk composition in larger herds of animals and various 
      breeds of sheep. (c) 2018 Society of Chemical Industry.
CI  - (c) 2018 Society of Chemical Industry.
FAU - Pecka-Kielb, Ewa
AU  - Pecka-Kielb E
AD  - Department of Biostructure and Animal Physiology, Wroclaw University of 
      Environmental and Life Sciences, Wroclaw, Poland.
FAU - Czerniawska-Piatkowska, Ewa
AU  - Czerniawska-Piatkowska E
AD  - Department of Genetics and Animal Breeding, West Pomeranian University of 
      Technology, Szczecin, Poland.
FAU - Kowalewska-Luczak, Inga
AU  - Kowalewska-Luczak I
AUID- ORCID: 0000-0001-5613-6079
AD  - Department of Genetics and Animal Breeding, West Pomeranian University of 
      Technology, Szczecin, Poland.
FAU - Vasil, Milan
AU  - Vasil M
AD  - Department of Epizootology and Parasitology, University of Veterinary Medicine 
      and Pharmacy, Kosice, Slovak Republic.
LA  - eng
PT  - Journal Article
DEP - 20180611
PL  - England
TA  - J Sci Food Agric
JT  - Journal of the science of food and agriculture
JID - 0376334
RN  - 0 (Annexins)
SB  - IM
MH  - Animals
MH  - Annexins/*genetics
MH  - Breeding
MH  - Female
MH  - Gene Frequency
MH  - Genotype
MH  - Male
MH  - Milk/*chemistry/metabolism
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polymorphism, Single Nucleotide
MH  - Sheep/*genetics/metabolism
OTO - NOTNLM
OT  - ANXA9 gene
OT  - milk
OT  - polymorphism
OT  - sheep
EDAT- 2018/04/18 06:00
MHDA- 2018/10/16 06:00
CRDT- 2018/04/18 06:00
PHST- 2016/09/06 00:00 [received]
PHST- 2018/04/06 00:00 [revised]
PHST- 2018/04/11 00:00 [accepted]
PHST- 2018/04/18 06:00 [pubmed]
PHST- 2018/10/16 06:00 [medline]
PHST- 2018/04/18 06:00 [entrez]
AID - 10.1002/jsfa.9081 [doi]
PST - ppublish
SO  - J Sci Food Agric. 2018 Nov;98(14):5396-5400. doi: 10.1002/jsfa.9081. Epub 2018 
      Jun 11.