PMID- 29663692
OWN - NLM
STAT- MEDLINE
DCOM- 20181108
LR  - 20181108
IS  - 1932-846X (Electronic)
IS  - 1932-8451 (Linking)
VI  - 78
IP  - 7
DP  - 2018 Jul
TI  - Profiles of gene expression in the hippocampal formation of rats with 
      experimentally-induced brain dysplasia.
PG  - 718-735
LID - 10.1002/dneu.22595 [doi]
AB  - Malformations of cortical development (MCD) are a common cause of intractable 
      seizures in humans. Among these, focal cortical dysplasia (FCD) poses an 
      outstanding challenge. There are several subtypes of FCD that show significant 
      variation in pathology and clinical presentation. All types exhibit disturbed 
      cortical cytoarchitecture and increased propensity for seizures. The etiology is 
      likely heterogenous, with mutations, specifically in genes related to mammalian 
      target of rapamycin (mTOR), identified in only a subset of cases. A more complex 
      mechanism, in which underlying genetic background interacts with early, pre- or 
      perinatal injury or stress, has been proposed. Here, we used a well-established 
      animal model of developmental malformations similar to MCD, induced by prenatal 
      gamma irradiation. Previously, a significant variation between times of treatment 
      has been shown, resulting in distinct and lasting patterns of dysplasia and 
      differentially altered seizure propensity. We set out to describe the molecular 
      background of these patterns by performing microarray analyses of hippocampal 
      samples obtained from adult rats previously irradiated at distinct time points 
      during gestation: E13, E15, E17 or E19 as well as controls. The analysis was 
      performed in three conditions: naive, during latent phase after 
      pilocarpine-induced status epilepticus and after 21 days of transauricular 
      electric shocks. A set of 22 transcripts, some with known functions related to 
      brain development, epilepsy and reaction to injury, was found to be altered 
      between these groups across all treatments. We discuss the functional implication 
      of these molecular differences, in an attempt to provide broader temporal and 
      developmental context. (c) 2018 Wiley Periodicals, Inc. Develop Neurobiol 78: 
      718-735, 2018.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Kielbinski, Michal
AU  - Kielbinski M
AUID- ORCID: 0000-0002-8825-179X
AD  - Department of Neuroanatomy, Institute of Zoology and Biomedical Research, 
      Jagiellonian University, Gronostajowa 9, Krakow, 30-387, Poland.
FAU - Setkowicz, Zuzanna
AU  - Setkowicz Z
AUID- ORCID: 0000-0002-7213-8194
AD  - Department of Neuroanatomy, Institute of Zoology and Biomedical Research, 
      Jagiellonian University, Gronostajowa 9, Krakow, 30-387, Poland.
FAU - Gzielo, Kinga
AU  - Gzielo K
AD  - Department of Neuroanatomy, Institute of Zoology and Biomedical Research, 
      Jagiellonian University, Gronostajowa 9, Krakow, 30-387, Poland.
FAU - Janeczko, Krzysztof
AU  - Janeczko K
AD  - Department of Neuroanatomy, Institute of Zoology and Biomedical Research, 
      Jagiellonian University, Gronostajowa 9, Krakow, 30-387, Poland.
LA  - eng
PT  - Journal Article
DEP - 20180421
PL  - United States
TA  - Dev Neurobiol
JT  - Developmental neurobiology
JID - 101300215
RN  - 01MI4Q9DI3 (Pilocarpine)
SB  - IM
MH  - Animals
MH  - Electroshock
MH  - Hippocampus/*growth & development/*metabolism
MH  - Male
MH  - Malformations of Cortical Development/*metabolism
MH  - Microarray Analysis
MH  - Pilocarpine
MH  - Polymerase Chain Reaction
MH  - Radiation Injuries, Experimental/metabolism
MH  - Random Allocation
MH  - Rats, Wistar
MH  - Status Epilepticus/*metabolism
OTO - NOTNLM
OT  - animal model
OT  - dysplasia
OT  - epilepsy
OT  - hippocampus
OT  - microarray
EDAT- 2018/04/18 06:00
MHDA- 2018/11/09 06:00
CRDT- 2018/04/18 06:00
PHST- 2017/11/15 00:00 [received]
PHST- 2018/03/28 00:00 [revised]
PHST- 2018/04/06 00:00 [accepted]
PHST- 2018/04/18 06:00 [pubmed]
PHST- 2018/11/09 06:00 [medline]
PHST- 2018/04/18 06:00 [entrez]
AID - 10.1002/dneu.22595 [doi]
PST - ppublish
SO  - Dev Neurobiol. 2018 Jul;78(7):718-735. doi: 10.1002/dneu.22595. Epub 2018 Apr 21.