PMID- 29663927
OWN - NLM
STAT- MEDLINE
DCOM- 20190424
LR  - 20190424
IS  - 1550-7033 (Print)
IS  - 1550-7033 (Linking)
VI  - 14
IP  - 3
DP  - 2018 Mar 1
TI  - Encapsulation of 5-Flurouracil into PLGA Nanofibers and Enhanced Anticancer 
      Effect in Combination with Ajwa-Dates-Extract (Phoenix dactylifera L.).
PG  - 553-563
LID - 10.1166/jbn.2018.2515 [doi]
AB  - Side effects connected with chemotherapeutic agents used in cancer treatment has 
      led to alternative modalities of combinatorial therapies in an attempt to reduce 
      the drug dosage and associated risks. In the current study we evaluated the 
      potential use of Ajwa Dates Extract (ADE), reported to have anti-cancer effects, 
      as an adjuvant therapy in combination with 5-flurouracil (5FU) against the 
      human-breast-adenocarcinoma cell line (MFC-7) in vitro. The effects of ADE alone 
      and in combination with 5-FU were evaluated in terms of cell viability and 
      cytotoxicity. For drug delivery purpose, we successfully encapsulated 5FU in both 
      presence and absence of ADE through electrospinning together with poly 
      lactic-co-glycolic acid (PLGA) in different combinations. Physicochemical 
      properties of 5FU and ADE incorporated into PLGA nanofibers remained unaltered as 
      confirmed by Fourier-Transform-Infrared (FTIR), Raman-spectroscopies and X-ray 
      Diffraction (XRD) techniques. The morphological characterization of nanofibers 
      was done using scanning electron microscopy (SEM) and atomic force microscopy 
      (AFM). The surface roughness of PLGA and PLGA + ADE nanofibers increased by 
      incorporation of 5FU. PLGA + ADE nanofibers were in hydrophilic range (<90 degrees ) 
      while nanofibers prepared from both PLGA + 5FU and PLGA + 5FU + ADE combinations 
      were in hydrophobic range ( approximately 112 degrees ). The percentage inhibition of MCF-7 
      proliferation at 72 hrs showed an enhanced combinatorial anti-cancer effect of 
      5FU and ADE on the cells seeded on PLGA + 5FU + ADE mat (47% decrease) while PLGA 
      + 5FU and PLGA + ADE demonstrated only 23% and 16% decrease respectively as 
      compared to controls. The hydrophobicity induced by 5FU can further be 
      investigated to get improved cellular adherence and efficient 
      controlled-drug-release.
FAU - Khan, Fazal
AU  - Khan F
FAU - Aldhahri, Musab
AU  - Aldhahri M
FAU - Hussain, Mohammad Asif
AU  - Hussain MA
FAU - Gauthaman, Kalamegam
AU  - Gauthaman K
FAU - Memic, Adnan
AU  - Memic A
FAU - Abuzenadah, Adel
AU  - Abuzenadah A
FAU - Kumosani, Taha
AU  - Kumosani T
FAU - Barbour, Elie
AU  - Barbour E
FAU - Alothmany, Nazeeh Shuja
AU  - Alothmany NS
FAU - Aldhaheri, Rabah Wasil
AU  - Aldhaheri RW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
RN  - 0 (Glycols)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
SB  - IM
MH  - Glycols
MH  - Humans
MH  - *Nanofibers
MH  - Phoeniceae
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
EDAT- 2018/04/18 06:00
MHDA- 2019/04/25 06:00
CRDT- 2018/04/18 06:00
PHST- 2018/04/18 06:00 [entrez]
PHST- 2018/04/18 06:00 [pubmed]
PHST- 2019/04/25 06:00 [medline]
AID - 10.1166/jbn.2018.2515 [doi]
PST - ppublish
SO  - J Biomed Nanotechnol. 2018 Mar 1;14(3):553-563. doi: 10.1166/jbn.2018.2515.