PMID- 29663988
OWN - NLM
STAT- MEDLINE
DCOM- 20181018
LR  - 20181018
IS  - 2095-4352 (Print)
VI  - 30
IP  - 4
DP  - 2018 Apr
TI  - [Unfractionated heparin inhibits the activation of endothelial cells by 
      decreasing the degree of HOXA9 reduced expression].
PG  - 302-305
LID - 10.3760/cma.j.issn.2095-4352.2018.04.003 [doi]
AB  - OBJECTIVE: To study unfractionated heparin (UFH) effect on the expression of 
      HOXA9 in activation of human umbilical vein endothelial cells (HUVECs) induced by 
      lipopolysaccharide (LPS). METHODS: HUVECs were cultured and they were randomly 
      divided into four groups (n = 5) for the challenge respectively: (1) control 
      group (with an equal volume of phosphate buffer saline); (2) LPS group (LPS 10 
      mg/L); (3) UFH group (UFH 10 kU/L); (4) UFH+LPS group (10 kU/L UFH 30 minutes + 
      LPS 10 mg/L). After treatment for 3 hours, the expressions of HOXA9, E-selectin 
      and nuclear factor-kappaB (NK-kappaB) in endothelial cells were detected by Western Blot. 
      RESULTS: Compared with the control group, the expression of HOXA9 in LPS group 
      was significantly decreased, the expressions of E-selectin and NF-kappaB were 
      significantly increased (HOXA9/beta-actin: 0.082+/-0.009 vs. 0.199+/-0.067, 
      E-selectin/beta-actin: 0.113+/-0.055 vs. 0.047+/-0.030, NF-kappaB/beta-actin: 0.845+/-0.025 vs. 
      0.664+/-0.092, all P < 0.05). Compared with LPS group, the expression of HOXA9 in 
      UFH+LPS group was significantly increased, the expressions of E-selectin and 
      NF-kappaB were significantly decreased (HOXA9/beta-actin: 0.190+/-0.096 vs. 0.082+/-0.009, 
      E-selecin/beta-actin: 0.057+/-0.017 vs. 0.113+/-0.055, NF-kappaB/beta-actin: 0.544+/-0.060 vs. 
      0.845+/-0.025, all P < 0.05). Each protein expression of UFH group were in 
      accordance with the control group. CONCLUSIONS: In LPS stimulated endothelial 
      cells, HOXA9 expression is down regulated, E-expression is reduced, and 
      endothelial cell activation is inhibited. UFH can inhibit the activation of 
      endothelial cells by decreasing the degree of HOXA9 reduced expression.
FAU - Yang, Rui
AU  - Yang R
AD  - Department of Critical Care Medicine, the First Hospital of China Medical 
      University, Shenyang 110001, Liaoning, China. Corresponding author: Ma Xiaochun, 
      Email: mxc2972@163.com.
FAU - Zhang, Xiaojuan
AU  - Zhang X
FAU - Liang, Yingjian
AU  - Liang Y
FAU - Ma, Xiaochun
AU  - Ma X
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhonghua wei zhong bing ji jiu yi xue
JID - 101604552
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (homeobox protein HOXA9)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Cells, Cultured
MH  - Heparin
MH  - Homeodomain Proteins
MH  - *Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Lipopolysaccharides
MH  - NF-kappa B
EDAT- 2018/04/18 06:00
MHDA- 2018/10/20 06:00
CRDT- 2018/04/18 06:00
PHST- 2018/04/18 06:00 [entrez]
PHST- 2018/04/18 06:00 [pubmed]
PHST- 2018/10/20 06:00 [medline]
AID - 10.3760/cma.j.issn.2095-4352.2018.04.003 [doi]
PST - ppublish
SO  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2018 Apr;30(4):302-305. doi: 
      10.3760/cma.j.issn.2095-4352.2018.04.003.