PMID- 29666957
OWN - NLM
STAT- MEDLINE
DCOM- 20180924
LR  - 20181202
IS  - 1573-6776 (Electronic)
IS  - 0141-5492 (Linking)
VI  - 40
IP  - 6
DP  - 2018 Jun
TI  - Conditioned medium from umbilical cord mesenchymal stem cells improves nasal 
      mucosa damage by radiation.
PG  - 999-1007
LID - 10.1007/s10529-018-2553-0 [doi]
AB  - OBJECTIVES: To explore therapeutic effects of conditioned medium from human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) on nasal mucosa radiation damage 
      both in vivo and in vitro. RESULTS: The mucus cilia clearance time (7 and 
      30 days), degree of mucosal edema (7, 30, 90 and 180 days), cilia coverage 
      (180 days) of concentrated conditioned medium group improved compared with 
      radiotherapy control group. The proliferation and migration abilities of 
      irradiated and non-irradiated nasal epithelial cells significantly increased 
      after culture in bronchial epithelial cell growth medium (BEGM) containing 10% 
      conditioned medium of hUC-MSCs compared to cells cultured in BEGM alone. 
      CONCLUSIONS: Soluble factors secreted by hUC-MSCs may promote nasal epithelial 
      cell proliferation and migration. Intranasal administration of hUC-MSC 
      conditioned medium effectively repairs nasal mucosa radiation damage.
FAU - Duan, Hong-Gang
AU  - Duan HG
AD  - Department of Otolaryngology, Second Affiliated Hospital, College of Medicine, 
      Zhejiang University, Hangzhou, China.
FAU - Ji, Fang
AU  - Ji F
AUID- ORCID: 0000-0002-0918-1173
AD  - Department of Neurology, The First Affiliated Hospital, Zhejiang University 
      School of Medicine, Qin-chun Road 79, Hangzhou, 310003, China. 
      1506065@zju.edu.cn.
FAU - Zheng, Chun-Quan
AU  - Zheng CQ
AD  - Department of Otolaryngology, Affiliated Eye and Ear, Nose and Throat Hospital, 
      Shanghai Medical College, Fudan University, Shanghai, China.
FAU - Li, Jing
AU  - Li J
AD  - Department of Otolaryngology, Hangzhou First People's Hospital, Hangzhou, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Otolaryngology, Second Affiliated Hospital, College of Medicine, 
      Zhejiang University, Hangzhou, China.
LA  - eng
GR  - 2016KYB124/Medicine and Health General Research Program of Zhejiang Province/
PT  - Journal Article
DEP - 20180417
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (Culture Media, Conditioned)
SB  - IM
MH  - Animals
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Cilia/drug effects
MH  - Culture Media, Conditioned/*pharmacology
MH  - Female
MH  - Guinea Pigs
MH  - Humans
MH  - Male
MH  - *Mesenchymal Stem Cells/cytology/metabolism
MH  - *Nasal Mucosa/cytology/drug effects/injuries/radiation effects
MH  - *Radiation Injuries
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - Conditioned medium
OT  - Cytokines
OT  - Mesenchymal stem cells
OT  - Nasal mucosa
OT  - Radiation damage
EDAT- 2018/04/19 06:00
MHDA- 2018/09/25 06:00
CRDT- 2018/04/19 06:00
PHST- 2017/12/03 00:00 [received]
PHST- 2018/04/06 00:00 [accepted]
PHST- 2018/04/19 06:00 [pubmed]
PHST- 2018/09/25 06:00 [medline]
PHST- 2018/04/19 06:00 [entrez]
AID - 10.1007/s10529-018-2553-0 [pii]
AID - 10.1007/s10529-018-2553-0 [doi]
PST - ppublish
SO  - Biotechnol Lett. 2018 Jun;40(6):999-1007. doi: 10.1007/s10529-018-2553-0. Epub 
      2018 Apr 17.