PMID- 29667321
OWN - NLM
STAT- MEDLINE
DCOM- 20180731
LR  - 20181202
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 33
IP  - 7
DP  - 2018 Jul
TI  - The ethanol extraction of prepared Psoralea corylifolia induces apoptosis and 
      autophagy and alteres genes expression assayed by cDNA microarray in human 
      prostate cancer PC-3 cells.
PG  - 770-788
LID - 10.1002/tox.22564 [doi]
AB  - Prostate cancer is the most common male reproductive system cancer. The 
      prevalence of prostate cancer in Europe and the United States is higher than that 
      in the Asian region. However, the treatment of prostate cancer remains 
      unsatisfactory. Psoralea corylifolia has been used to cure this disease as 
      Chinese medicine in the Asian region. In this study, we analyzed the components 
      of ethanol extraction of unprepared and prepared P. corylifolia by HPLC. Psoralen 
      and isopsoralen content from the prepared P. corylifolia is twofold higher than 
      that from unprepared, so we use the prepared extraction in this study. However, 
      the effects of the ethanol extraction of P. corylifolia (PCE) on PC-3 human 
      prostate cancer cells remain unclear. PC-3 cells were treated with PCE for 
      different time periods and cells were examined for cell morphological change and 
      total viable cells by using contrast phase microscopy and flow cytometer, 
      respectively. Results indicated that PCE induced cell morphological changes and 
      cytotoxic effect in PC-3 cells in dose-dependent manners. PCE induced chromatin 
      condensation of PC-3 cells dose-dependently. PCE also induced apoptosis and 
      autophagy in PC-3 by western blotting and acridine orange (AO) staining, 
      respectively. Furthermore, a complementary DNA microarray analysis demonstrated 
      that PCE treatment led to 944 genes upregulation and 872 genes downregulation. 
      For example, the DNA damage-associated gene DNA-damage-inducible transcript 3 
      (DDIT 3) had a 62.1-fold upregulation and CDK1 2.68-fold downregulation. The 
      differential genes were classified according to the Gene Ontology. Furthermore, 
      GeneGo software was used for the key genes involved and their possible 
      interaction pathways. Those genes were affected by P. corylifolia, which provided 
      information for the understanding of the antiprostate cancer mechanism at the 
      genetic level and provide additional targets for the treatments of human prostate 
      cancer.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Lin, Chia-Hsin
AU  - Lin CH
AD  - Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, 
      China Medical University, Taichung, 404, Taiwan.
FAU - Funayama, Shinji
AU  - Funayama S
AD  - Department of Kampo Pharmaceutical Sciences, Nihon Pharmaceutical University 
      Saitama, Saitama, Japan.
FAU - Peng, Shu-Fen
AU  - Peng SF
AD  - Department of Medical Research, China Medical University Hospital, China Medical 
      University, Taichung, 404, Taiwan.
FAU - Kuo, Chao-Lin
AU  - Kuo CL
AD  - Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, 
      China Medical University, Taichung, 404, Taiwan.
FAU - Chung, Jing-Gung
AU  - Chung JG
AUID- ORCID: 0000-0002-7303-2287
AD  - Department of Biological Science and Technology, China Medical University, 404, 
      Taiwan, Taichung.
AD  - Department of Biotechnology, Asia University, Taichung, 413, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20180418
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - 0 (Furocoumarins)
RN  - 0 (Plant Extracts)
RN  - 147336-12-7 (Transcription Factor CHOP)
RN  - 3K9958V90M (Ethanol)
RN  - CZZ080D7BD (angelicin)
RN  - EC 2.7.11.22 (CDC2 Protein Kinase)
RN  - KTZ7ZCN2EX (Ficusin)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Autophagy/*drug effects
MH  - CDC2 Protein Kinase/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Down-Regulation/drug effects
MH  - Ethanol/chemistry
MH  - Ficusin/chemistry/isolation & purification/pharmacology
MH  - Furocoumarins/chemistry/isolation & purification/pharmacology
MH  - Humans
MH  - Male
MH  - Oligonucleotide Array Sequence Analysis
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Prostatic Neoplasms/metabolism/pathology
MH  - Psoralea/*chemistry/metabolism
MH  - Transcription Factor CHOP/genetics/metabolism
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - PC-3
OT  - Psoralea corylifolia
OT  - apoptosis
OT  - autophagy
OT  - prostate cancer
EDAT- 2018/04/19 06:00
MHDA- 2018/08/01 06:00
CRDT- 2018/04/19 06:00
PHST- 2017/09/18 00:00 [received]
PHST- 2018/03/25 00:00 [revised]
PHST- 2018/04/01 00:00 [accepted]
PHST- 2018/04/19 06:00 [pubmed]
PHST- 2018/08/01 06:00 [medline]
PHST- 2018/04/19 06:00 [entrez]
AID - 10.1002/tox.22564 [doi]
PST - ppublish
SO  - Environ Toxicol. 2018 Jul;33(7):770-788. doi: 10.1002/tox.22564. Epub 2018 Apr 
      18.