PMID- 29667550
OWN - NLM
STAT- MEDLINE
DCOM- 20181003
LR  - 20190318
IS  - 1873-4316 (Electronic)
IS  - 1389-2010 (Linking)
VI  - 19
IP  - 1
DP  - 2018
TI  - In vitro Studies on Cytotoxic, DNA Protecting, Antibiofilm and Antibacterial 
      Effects of Biogenic Silver Nanoparticles Prepared with Bergenia ciliata Rhizome 
      Extract.
PG  - 68-78
LID - 10.2174/1389201019666180417160049 [doi]
AB  - BACKGROUND: Many health hazardous diseases are caused by clinical pathogens. 
      Drugresistant microbes are one of the major health problems in the world. To 
      overcome the effect of infectious diseases new antimicrobial agent from nature 
      has been explored which is environmentally friendly, less costly and more 
      effective for the development of next-generation drugs. Bergenia ciliata and 
      silver nitrate both have medicinal properties. OBJECTIVES: The aim of the current 
      research was to evaluate the cytotoxic, and antibacterial effect of green 
      synthesized nanoparticles using Bergenia ciliata rhizome against clinical 
      bacterial pathogens. METHODS: Extract of Bergenia ciliata was prepared by 
      maceration technique. Silver nanoparticles were synthesized using Bergenia 
      ciliata rhizome extract. Synthesized silver nanoparticles were confirmed by 
      UV-vis spectrophotometer, Scanning electron microscope (SEM) and Fourier 
      Transform Infrared Spectroscopy (FTIR). The antibacterial, anti-biofilm, cell 
      proliferation inhibition, DNA protection, brine shrimp lethality effects of 
      synthesized nanoparticles were investigated. RESULTS: UV-vis spectrophotometer 
      indicated the prelaminar synthesis of silver nanoparticles at 400 nm. The 
      spherical shape of synthesized nanoparticles with 35 nm size was confirmed using 
      SEM. Greatest zone of inhibition (6.0 +/- 0.0 mm to 8.3 +/- 0.57 mm) was recorded 
      against all tested pathogens compared with the B. ciliata aqueous extract. 
      Anti-biofilm analysis and MTT assay supported the results of the antibacterial 
      activity. Silver nanoparticles protect the DNA degradation. CONCLUSION: Green 
      synthesized nanoparticles had potent antibacterial activity and may provide a 
      basis for the development of the new antibacterial drug.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.org.
FAU - Zia, Ghazna
AU  - Zia G
AD  - Microbial Biotechnology Laboratory, Department of Zoology, University of Azad 
      Jammu and Kashmir, Muzaffarabad, 13100, Pakistan.
FAU - Sadia, Haleema
AU  - Sadia H
AD  - Microbial Biotechnology Laboratory, Department of Zoology, University of Azad 
      Jammu and Kashmir, Muzaffarabad, 13100, Pakistan.
FAU - Nazir, Shabnam
AU  - Nazir S
AD  - Microbial Biotechnology Laboratory, Department of Zoology, University of Azad 
      Jammu and Kashmir, Muzaffarabad, 13100, Pakistan.
FAU - Ejaz, Kiren
AU  - Ejaz K
AD  - Microbial Biotechnology Laboratory, Department of Zoology, University of Azad 
      Jammu and Kashmir, Muzaffarabad, 13100, Pakistan.
FAU - Ali, Shaukat
AU  - Ali S
AD  - Microbial Biotechnology Laboratory, Department of Zoology, University of Azad 
      Jammu and Kashmir, Muzaffarabad, 13100, Pakistan.
FAU - Ihsan-Ul-Haq
AU  - Ihsan-Ul-Haq
AD  - Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan.
FAU - Iqbal, Tariq
AU  - Iqbal T
AD  - Department of Physics, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, 
      Pakistan.
FAU - Khan, Muhammad A R
AU  - Khan MAR
AD  - Department of Physics, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, 
      Pakistan.
FAU - Raza, Abida
AU  - Raza A
AD  - National Institute for Lasers and Optronics (NILOP), Pakistan Atomic Energy 
      Commission, Islamabad, Pakistan.
FAU - Andleeb, Saiqa
AU  - Andleeb S
AD  - Microbial Biotechnology Laboratory, Department of Zoology, University of Azad 
      Jammu and Kashmir, Muzaffarabad, 13100, Pakistan.
LA  - eng
PT  - Letter
PL  - Netherlands
TA  - Curr Pharm Biotechnol
JT  - Current pharmaceutical biotechnology
JID - 100960530
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Plant Extracts)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/chemistry/isolation & purification/*pharmacology
MH  - Artemia/drug effects
MH  - Bacteria/drug effects/growth & development
MH  - Biofilms/*drug effects/growth & development
MH  - DNA Fragmentation/*drug effects
MH  - Green Chemistry Technology/methods
MH  - Humans
MH  - Metal Nanoparticles/*administration & dosage/chemistry
MH  - Plant Extracts/chemistry/isolation & purification/*pharmacology
MH  - Plants, Medicinal
MH  - Rhizome
MH  - Silver/*pharmacology
MH  - Spectroscopy, Fourier Transform Infrared/methods
OTO - NOTNLM
OT  - Anti-biofilm assay
OT  - FTIR
OT  - UV-vis spectrophotometer
OT  - antibacterial assay
OT  - brine shrimp assay
OT  - cell viability assay
OT  - protein kinase inhibitor assay
OT  - scanning electron microscope.
EDAT- 2018/04/19 06:00
MHDA- 2018/10/04 06:00
CRDT- 2018/04/19 06:00
PHST- 2017/04/10 00:00 [received]
PHST- 2017/11/05 00:00 [revised]
PHST- 2018/04/16 00:00 [accepted]
PHST- 2018/04/19 06:00 [pubmed]
PHST- 2018/10/04 06:00 [medline]
PHST- 2018/04/19 06:00 [entrez]
AID - CPB-EPUB-89790 [pii]
AID - 10.2174/1389201019666180417160049 [doi]
PST - ppublish
SO  - Curr Pharm Biotechnol. 2018;19(1):68-78. doi: 10.2174/1389201019666180417160049.