PMID- 29671751
OWN - NLM
STAT- MEDLINE
DCOM- 20190906
LR  - 20190906
IS  - 1361-6528 (Electronic)
IS  - 0957-4484 (Linking)
VI  - 29
IP  - 28
DP  - 2018 Jul 13
TI  - Single molecule localization imaging of telomeres and centromeres using 
      fluorescence in situ hybridization and semiconductor quantum dots.
PG  - 285602
LID - 10.1088/1361-6528/aabf72 [doi]
AB  - Single molecule localization microscopy (SMLM) is a powerful tool for imaging 
      biological targets at the nanoscale. In this report, we present SMLM imaging of 
      telomeres and centromeres using fluorescence in situ hybridization (FISH). The 
      FISH probes were fabricated by decorating CdSSe/ZnS quantum dots (QDs) with 
      telomere or centromere complementary DNA strands. SMLM imaging experiments using 
      commercially available peptide nucleic acid (PNA) probes labeled with organic 
      fluorophores were also conducted to demonstrate the advantages of using QDs FISH 
      probes. Compared with the PNA probes, the QDs probes have the following merits. 
      First, the fluorescence blinking of QDs can be realized in aqueous solution or 
      PBS buffer without thiol, which is a key buffer component for organic 
      fluorophores' blinking. Second, fluorescence blinking of the QDs probe needs only 
      one excitation light (i.e. 405 nm). While fluorescence blinking of the organic 
      fluorophores usually requires two illumination lights, that is, the activation 
      light (i.e. 405 nm) and the imaging light. Third, the high quantum yield, 
      multiple switching times and a good optical stability make the QDs more suitable 
      for long-term imaging. The localization precision achieved in telomeres and 
      centromeres imaging experiments is about 30 nm, which is far beyond the 
      diffraction limit. SMLM has enabled new insights into telomeres or centromeres on 
      the molecular level, and it is even possible to determine the length of telomere 
      and become a potential technique for telomere-related investigation.
FAU - Wang, Le
AU  - Wang L
AD  - Advanced Photonics Center, Southeast University, Nanjing 210096, Jiangsu, 
      People's Republic of China.
FAU - Zong, Shenfei
AU  - Zong S
FAU - Wang, Zhuyuan
AU  - Wang Z
FAU - Lu, Ju
AU  - Lu J
FAU - Chen, Chen
AU  - Chen C
FAU - Zhang, Ruohu
AU  - Zhang R
FAU - Cui, Yiping
AU  - Cui Y
LA  - eng
PT  - Journal Article
DEP - 20180419
PL  - England
TA  - Nanotechnology
JT  - Nanotechnology
JID - 101241272
RN  - 0 (Cadmium Compounds)
RN  - 0 (Molecular Probes)
RN  - 0 (Sulfides)
RN  - 0 (Zinc Compounds)
RN  - 057EZR4Z7Q (cadmium sulfide)
RN  - KPS085631O (zinc sulfide)
SB  - IM
MH  - Cadmium Compounds/chemistry
MH  - Centromere/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lasers
MH  - Molecular Probes/chemistry
MH  - Quantum Dots/*chemistry/ultrastructure
MH  - *Semiconductors
MH  - Single Molecule Imaging/*methods
MH  - Sulfides/chemistry
MH  - Telomere/*metabolism
MH  - Zinc Compounds/chemistry
EDAT- 2018/04/20 06:00
MHDA- 2019/09/07 06:00
CRDT- 2018/04/20 06:00
PHST- 2018/04/20 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
PHST- 2018/04/20 06:00 [entrez]
AID - 10.1088/1361-6528/aabf72 [doi]
PST - ppublish
SO  - Nanotechnology. 2018 Jul 13;29(28):285602. doi: 10.1088/1361-6528/aabf72. Epub 
      2018 Apr 19.