PMID- 29672206
OWN - NLM
STAT- MEDLINE
DCOM- 20190730
LR  - 20240329
IS  - 1551-5044 (Electronic)
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 66
IP  - 8
DP  - 2018 Aug
TI  - Full Karyotype Interphase Cell Analysis.
PG  - 595-606
LID - 10.1369/0022155418771613 [doi]
AB  - Aneuploidy seems to play not only a decisive role in embryonal development but 
      also in tumorigenesis where chromosomal and genomic instability reflect a 
      universal feature of malignant tumors. The cost of whole genome sequencing has 
      fallen significantly, but it is still prohibitive for many institutions and 
      clinical settings. No applied, cost-effective, and efficient technique has been 
      introduced yet aiming at research to assess the ploidy status of all 24 different 
      human chromosomes in interphases simultaneously, especially in single cells. 
      Here, we present the selection of human probe DNA and a technique using multistep 
      fluorescence in situ hybridization (FISH) employing four sets of six labeled FISH 
      probes able to delineate all 24 human chromosomes in interphase cells. This full 
      karyotype analysis approach will provide additional diagnostic potential for 
      single cell analysis. The use of spectral imaging (SIm) has enabled the use of up 
      to eight different fluorochrome labels simultaneously. Thus, scoring can be 
      easily assessed by visual inspection, because SIm permits computer-assigned and 
      distinguishable pseudo-colors to each probe during image processing. This enables 
      full karyotype analysis by FISH of single-cell interphase nuclei.
FAU - Baumgartner, Adi
AU  - Baumgartner A
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, University of 
      California, San Francisco, California.
AD  - Life Sciences Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, 
      California.
AD  - Biomedical Science, School of Health Sciences, York St John University, York, 
      United Kingdom.
FAU - Ferlatte Hartshorne, Christy
AU  - Ferlatte Hartshorne C
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, University of 
      California, San Francisco, California.
FAU - Polyzos, Aris A
AU  - Polyzos AA
AD  - Life Sciences Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, 
      California.
FAU - Weier, Heinz-Ulrich G
AU  - Weier HG
AD  - Life Sciences Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, 
      California.
FAU - Weier, Jingly Fung
AU  - Weier JF
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, University of 
      California, San Francisco, California.
AD  - Dermatopathology Service, University of California, San Francisco, California.
AD  - Life Sciences Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, 
      California.
FAU - O'Brien, Ben
AU  - O'Brien B
AUID- ORCID: 0000-0001-7024-2337
AD  - Life Sciences Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, 
      California.
AD  - Department of Perioperative Medicine, St Bartholomew's Hospital & Barts Heart 
      Centre, London, United Kingdom.
AD  - Outcomes Research Consortium, Cleveland Clinic, Cleveland, Ohio.
LA  - eng
GR  - K25 HD041425/HD/NICHD NIH HHS/United States
GR  - R01 HD045736/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20180419
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (DNA Probes)
MH  - *Aneuploidy
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - DNA Probes/genetics
MH  - Humans
MH  - Image Processing, Computer-Assisted/methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Interphase
MH  - *Karyotype
MH  - Karyotyping/*methods
MH  - Male
MH  - Plasmids/genetics
MH  - Single-Cell Analysis/methods
PMC - PMC6071177
OTO - NOTNLM
OT  - DNA probes
OT  - FISH
OT  - aneuploidy
OT  - chromosome enumeration
OT  - full karyotype analysis
OT  - interphase nuclei
OT  - spectral imaging
COIS- Competing Interests: The author(s) declared no potential conflicts of interest 
      with respect to the research, authorship, and/or publication of this article.
EDAT- 2018/04/20 06:00
MHDA- 2019/07/31 06:00
PMCR- 2019/08/01
CRDT- 2018/04/20 06:00
PHST- 2018/04/20 06:00 [pubmed]
PHST- 2019/07/31 06:00 [medline]
PHST- 2018/04/20 06:00 [entrez]
PHST- 2019/08/01 00:00 [pmc-release]
AID - 10.1369_0022155418771613 [pii]
AID - 10.1369/0022155418771613 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2018 Aug;66(8):595-606. doi: 10.1369/0022155418771613. Epub 
      2018 Apr 19.