PMID- 29672782
OWN - NLM
STAT- MEDLINE
DCOM- 20190618
LR  - 20190618
IS  - 1439-3980 (Electronic)
IS  - 0722-1819 (Linking)
VI  - 50
IP  - 2
DP  - 2018 Apr
TI  - [Comparison of the regenerative cytokine profile of adipose-derived stromal cells 
      (ASCs) harvested via abdominoplasty, suction-assisted liposuction (SAL) and 
      ultrasound-assisted liposuction (UAL)].
PG  - 74-82
LID - 10.1055/s-0043-118596 [doi]
AB  - INTRODUCTION: Mesenchymal stem cells are already used in numerous areas of 
      medicine. In the field of plastic surgery, the main focus of recent research has 
      been to utilise adipose-derived stromal cells (ASCs), mainly harvested via 
      liposuction. The ability to release regenerative cytokines is supposed to be the 
      key mechanism for the therapeutic efficacy of ASCs. There are currently a variety 
      of methods and devices in clinical use for harvesting lipoaspirate and 
      consequently ASCs. For most of these devices, the effect on the regenerative 
      cytokine profile of ASCs is unknown. The aim of this study is to compare the 
      expression of key regenerative cytokines of ASCs derived by three standard 
      procedures of plastic surgery: abdominoplasty, suction-assisted liposuction (SAL) 
      and ultrasound-assisted liposuction (UAL). PATIENTS AND METHODS: UAL and SAL 
      lipoaspirates were obtained from 3 healthy female volunteers before 
      abdominoplasty was performed in the same patients. ASCs were isolated from UAL 
      and SAL lipoaspirates as well as from abdominoplasty resections and RNA 
      expression of important regenerative cytokines (HGF (hepatocyte growth factor), 
      FGF-2 (basic fibroblast growth factor), MCP-1 (monocyte chemotactic protein 1), 
      SDF-1 (stromal cell-derived factor 1) and VEGF (vascular endothelial growth 
      factor) was compared. RESULTS: There was no difference in the expression of HGF, 
      FGF-2, SDF-1 and VEGF between the individual samples, but we were able to 
      demonstrate significantly increased expression of MCP-1 in ASCs obtained from UAL 
      lipoaspirate. CONCLUSION: UAL and SAL lipoaspirates are suitable for obtaining 
      functional ASCs and are comparable to minimally manipulated cells from 
      abdominoplasty resections.
CI  - (c) Georg Thieme Verlag KG Stuttgart . New York.
FAU - Aitzetmuller, Matthias Michael
AU  - Aitzetmuller MM
AD  - Abteilung fur Experimentelle Plastische Chirugie, Klinik fur Plastische Chirurgie 
      und Handchirugie, Klinikum rechts der Isar der Technischen Universitat Munchen.
FAU - Machens, Hans-Gunther
AU  - Machens HG
AD  - Abteilung fur Experimentelle Plastische Chirugie, Klinik fur Plastische Chirurgie 
      und Handchirugie, Klinikum rechts der Isar der Technischen Universitat Munchen.
FAU - Schilling, Arndt F
AU  - Schilling AF
AD  - Abteilung fur Forschung und Entwicklung, Klinik fur Unfallchirurgie, Orthopadie 
      und Plastische Chirurgie Universitatsklinikum Gottingen.
FAU - Duscher, Dominik
AU  - Duscher D
AD  - Abteilung fur Experimentelle Plastische Chirugie, Klinik fur Plastische Chirurgie 
      und Handchirugie, Klinikum rechts der Isar der Technischen Universitat Munchen.
LA  - ger
PT  - Journal Article
TT  - Vergleich des Regenerativen Zytokinprofils von Adipose Derived Stromal Cells 
      (ASCs) Gewonnen Mittels Abdominoplastik, Suction Assisted Liposuction (SAL) und 
      Ultrasound Assisted Liposuction (UAL).
DEP - 20180419
PL  - Germany
TA  - Handchir Mikrochir Plast Chir
JT  - Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen 
      Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen 
      Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ 
      der V...
JID - 8302815
RN  - 0 (Cytokines)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - *Abdominoplasty
MH  - *Adipocytes
MH  - *Adipose Tissue/cytology
MH  - *Cytokines/analysis
MH  - Female
MH  - Humans
MH  - *Lipectomy
MH  - *Mesenchymal Stem Cells
MH  - Suction
MH  - Surgery, Plastic
MH  - Vascular Endothelial Growth Factor A
COIS- Die Autoren geben an, dass kein Interessenkonflikt besteht.
EDAT- 2018/04/20 06:00
MHDA- 2019/06/19 06:00
CRDT- 2018/04/20 06:00
PHST- 2018/04/20 06:00 [entrez]
PHST- 2018/04/20 06:00 [pubmed]
PHST- 2019/06/19 06:00 [medline]
AID - 10.1055/s-0043-118596 [doi]
PST - ppublish
SO  - Handchir Mikrochir Plast Chir. 2018 Apr;50(2):74-82. doi: 10.1055/s-0043-118596. 
      Epub 2018 Apr 19.