PMID- 29673101
OWN - NLM
STAT- MEDLINE
DCOM- 20191025
LR  - 20240327
IS  - 2045-7634 (Electronic)
IS  - 2045-7634 (Linking)
VI  - 7
IP  - 6
DP  - 2018 Jun
TI  - Identification of four plasma microRNAs as potential biomarkers in the diagnosis 
      of male lung squamous cell carcinoma patients in China.
PG  - 2370-2381
LID - 10.1002/cam4.1490 [doi]
AB  - Dysregulated microRNAs (miRNAs) in the plasma of patients with lung squamous cell 
      carcinoma (LSCC) might serve as biomarkers for LSCC diagnosis. The expression of 
      miRNAs was performed using quantitative reverse transcription-polymerase chain 
      reaction (qRT-PCR) on the basis of Exiqon panels in the initial screening phase 
      including three male LSCC pool samples and one normal control (NC) pool sample 
      (per 10 samples were pooled as one pool sample). After the training (32 LSCC vs. 
      31 NCs), the testing (55 LSCC vs. 55 NCs), and the external validation (15 LSCC 
      vs. 15 NCs) stages via qRT-PCR, a four-miRNA signature (miR-181a-5p, miR-21-5p, 
      miR-106a-5p, and miR-93-5p) was identified for LSCC detection. Areas under the 
      receiver operating characteristic (ROC) curve (AUC) of the four-miRNA panel for 
      the training, the testing, and the external validation phases were 0.795, 0.827, 
      and 0.914, respectively. Then, the four miRNAs were explored in LSCC tissue 
      samples (23 LSCC vs. 23 NCs), and their expression was significantly 
      up-regulated. However, none of the four miRNAs found significantly up-regulated 
      in plasma exosomes expect miR-93-5p with borderline significance (16 LSCC vs. 16 
      NCs). In summary, our study established a four-miRNA peripheral plasma signature, 
      which contributed to diagnosing male LSCC patients in China to a certain degree.
CI  - (c) 2018 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.
FAU - Shan, Xia
AU  - Shan X
AD  - Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 
      300 Guangzhou Road, Nanjing, 210029, China.
AD  - Department of Respiration, The Affiliated Jiangning Hospital of Nanjing Medical 
      University, Nanjing, 210000, China.
FAU - Zhang, Huo
AU  - Zhang H
AD  - Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 
      300 Guangzhou Road, Nanjing, 210029, China.
FAU - Zhang, Lan
AU  - Zhang L
AD  - Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 
      300 Guangzhou Road, Nanjing, 210029, China.
AD  - Department of Radiation Oncology, Suzhou Municipal Hospital, Suzhou Cancer 
      Center, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, 
      Jiangsu, 215001, China.
FAU - Zhou, Xin
AU  - Zhou X
AUID- ORCID: 0000-0002-6566-9018
AD  - Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 
      300 Guangzhou Road, Nanjing, 210029, China.
FAU - Wang, Tongshan
AU  - Wang T
AD  - Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 
      300 Guangzhou Road, Nanjing, 210029, China.
FAU - Zhang, JinYing
AU  - Zhang J
AD  - Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 
      300 Guangzhou Road, Nanjing, 210029, China.
FAU - Shu, Yongqian
AU  - Shu Y
AD  - Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 
      300 Guangzhou Road, Nanjing, 210029, China.
FAU - Zhu, Wei
AU  - Zhu W
AUID- ORCID: 0000-0002-5744-5962
AD  - Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 
      300 Guangzhou Road, Nanjing, 210029, China.
AD  - Department of Oncology, The Affiliated Jiangsu Shengze Hospital of Nanjing 
      Medical University, No.1399 West Road, Wujiang District, Suzhou 215000, China.
FAU - Wen, Wei
AU  - Wen W
AD  - Department of Thoracic Surgery, First Affiliated Hospital of Nanjing Medical 
      University, 300 Guangzhou Road, Nanjing 210029, China.
FAU - Liu, Ping
AU  - Liu P
AD  - Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 
      300 Guangzhou Road, Nanjing, 210029, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180419
PL  - United States
TA  - Cancer Med
JT  - Cancer medicine
JID - 101595310
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - China
MH  - Humans
MH  - Lung Neoplasms/*genetics/pathology
MH  - Male
MH  - MicroRNAs/*genetics
PMC - PMC6010830
OTO - NOTNLM
OT  - Diagnostic biomarker
OT  - lung squamous cell carcinoma
OT  - microRNA
OT  - plasma
OT  - qRT-PCR
EDAT- 2018/04/20 06:00
MHDA- 2019/10/28 06:00
PMCR- 2018/04/19
CRDT- 2018/04/20 06:00
PHST- 2018/01/15 00:00 [received]
PHST- 2018/03/15 00:00 [revised]
PHST- 2018/03/20 00:00 [accepted]
PHST- 2018/04/20 06:00 [pubmed]
PHST- 2019/10/28 06:00 [medline]
PHST- 2018/04/20 06:00 [entrez]
PHST- 2018/04/19 00:00 [pmc-release]
AID - CAM41490 [pii]
AID - 10.1002/cam4.1490 [doi]
PST - ppublish
SO  - Cancer Med. 2018 Jun;7(6):2370-2381. doi: 10.1002/cam4.1490. Epub 2018 Apr 19.