PMID- 29673661
OWN - NLM
STAT- MEDLINE
DCOM- 20181002
LR  - 20220409
IS  - 1875-6263 (Electronic)
IS  - 1028-4559 (Linking)
VI  - 57
IP  - 2
DP  - 2018 Apr
TI  - Vitamin C attenuates negative effects of vitrification on sperm parameters, 
      chromatin quality, apoptosis and acrosome reaction in neat and prepared 
      normozoospermic samples.
PG  - 200-204
LID - S1028-4559(18)30024-X [pii]
LID - 10.1016/j.tjog.2018.02.006 [doi]
AB  - OBJECTIVE: Aim of this study was to evaluate the effects of vitamin C on sperm 
      parameters, sperm chromatin quality and apoptosis resulted of vitrification in 
      neat semen and prepared spermatozoa of normozoospermic samples. MATERIAL AND 
      METHODS: Forty semen samples from normozoospermic men were included in this 
      prospective study. Each sample was divided into five groups. Group I: control or 
      fresh semen, group II: semen prepared by swim-up method and then vitrified, group 
      III: neat semen was vitrified, group IV: vitamin C (600 muM) was added to prepared 
      spermatozoa and then vitrified and group V: vitamin C (600 muM) was added to neat 
      semen and then vitrified. After warming, sperm analysis was done accordingly. For 
      evaluating the sperm chromatin/DNA integrity status and acrosome reaction, we 
      used toluidine blue (TB), acridine orange (AO), terminal transferase mediated 
      deoxyuridine triphosphate biotin end labeling (TUNEL) and double staining tests. 
      RESULTS: All of the sperm parameters (count, motility, morphology and viability) 
      had significant differences (P < 0.05) between different groups, especially in 
      group IV. Data showed sperm chromatin damages and acrosome reaction abnormality 
      increased resulted of vitrification, but, in the groups that added vitamin C (IV, 
      V) rate of damages was decreased and this was notable in the group IV. 
      CONCLUSION: Vitamin C can attenuate the detrimental effects of vitrification on 
      sperm parameters, chromatin quality and rate of apoptosis in both neat semen and 
      prepared spermatozoa of normozoospermic samples.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - Mangoli, Esmat
AU  - Mangoli E
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Talebi, Ali Reza
AU  - Talebi AR
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran. Electronic address: prof_talebi@Hotmail.com.
FAU - Anvari, Morteza
AU  - Anvari M
AD  - Department of Biology, Shahid Sadoughi University of Medical Sciences, Yazd, 
      Iran; Department of Anatomical Sciences, Shahid Sadoughi University of Medical 
      Sciences, Yazd, Iran.
FAU - Taheri, Fatemeh
AU  - Taheri F
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Vatanparast, Mahboobeh
AU  - Vatanparast M
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Rahiminia, Tahereh
AU  - Rahiminia T
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Hosseini, Akram
AU  - Hosseini A
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Taiwan J Obstet Gynecol
JT  - Taiwanese journal of obstetrics & gynecology
JID - 101213819
RN  - 0 (Chromatin)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Acrosome Reaction/*drug effects
MH  - Apoptosis/*drug effects
MH  - Ascorbic Acid/*pharmacology
MH  - Cell Survival/drug effects
MH  - Chromatin/*drug effects
MH  - Cryopreservation/*methods
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Semen Preservation/methods
MH  - Sperm Count
MH  - Sperm Motility
MH  - Spermatozoa/*drug effects/physiology
OTO - NOTNLM
OT  - Chromatin
OT  - Human sperm
OT  - Spermatozoa
OT  - Vitamin C
OT  - Vitrification
EDAT- 2018/04/21 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/04/21 06:00
PHST- 2017/10/05 00:00 [accepted]
PHST- 2018/04/21 06:00 [entrez]
PHST- 2018/04/21 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
AID - S1028-4559(18)30024-X [pii]
AID - 10.1016/j.tjog.2018.02.006 [doi]
PST - ppublish
SO  - Taiwan J Obstet Gynecol. 2018 Apr;57(2):200-204. doi: 10.1016/j.tjog.2018.02.006.