PMID- 29675316
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220129
IS  - 2156-7085 (Print)
IS  - 2156-7085 (Electronic)
IS  - 2156-7085 (Linking)
VI  - 9
IP  - 4
DP  - 2018 Apr 1
TI  - Widefield standing wave microscopy of red blood cell membrane morphology with 
      high temporal resolution.
PG  - 1745-1761
LID - 10.1364/BOE.9.001745 [doi]
AB  - We report the first demonstration of widefield standing wave (SW) microscopy of 
      fluorescently labelled red blood cells at high speeds that allow for the rapid 
      imaging of membrane deformations. Using existing and custom MATLAB functions, we 
      also present a method to generate 2D and 3D reconstructions of the SW data for 
      improved visualization of the cell. We compare our technique with standard 
      widefield epifluorescence imaging and show that the SW technique not only reveals 
      more topographical information about the specimen but does so without increasing 
      toxicity or the rate of photobleaching and could make this a powerful technique 
      for the diagnosis or study of red blood cell morphology and biomechanical 
      characteristics.
FAU - Tinning, Peter W
AU  - Tinning PW
AD  - Department of Physics, SUPA, University of Strathclyde, Glasgow, G4 ONG, UK.
AD  - peter.tinning@strath.ac.uk.
FAU - Scrimgeour, Ross
AU  - Scrimgeour R
AD  - Department of Physics, SUPA, University of Strathclyde, Glasgow, G4 ONG, UK.
AD  - ross.scrimgeour@strath.ac.uk.
FAU - McConnell, Gail
AU  - McConnell G
AD  - Department of Physics, SUPA, University of Strathclyde, Glasgow, G4 ONG, UK.
LA  - eng
GR  - MR/K015583/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20180316
PL  - United States
TA  - Biomed Opt Express
JT  - Biomedical optics express
JID - 101540630
PMC - PMC5905920
OTO - NOTNLM
OT  - (100.3020) Image reconstruction-restoration
OT  - (170.0180) Microscopy
OT  - (170.2520) Fluorescence microscopy
OT  - (170.3880) Medical and biological imaging
OT  - (230.3670) Light-emitting diodes
COIS- The authors declare that there are no conflicts of interest related to this 
      article.
EDAT- 2018/04/21 06:00
MHDA- 2018/04/21 06:01
PMCR- 2018/03/16
CRDT- 2018/04/21 06:00
PHST- 2017/12/26 00:00 [received]
PHST- 2018/03/09 00:00 [revised]
PHST- 2018/03/09 00:00 [accepted]
PHST- 2018/04/21 06:00 [entrez]
PHST- 2018/04/21 06:00 [pubmed]
PHST- 2018/04/21 06:01 [medline]
PHST- 2018/03/16 00:00 [pmc-release]
AID - 318125 [pii]
AID - 10.1364/BOE.9.001745 [doi]
PST - epublish
SO  - Biomed Opt Express. 2018 Mar 16;9(4):1745-1761. doi: 10.1364/BOE.9.001745. 
      eCollection 2018 Apr 1.