PMID- 29675336
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2162-1918 (Print)
IS  - 2162-1934 (Electronic)
IS  - 2162-1918 (Linking)
VI  - 7
IP  - 4
DP  - 2018 Apr 1
TI  - Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds.
PG  - 105-113
LID - 10.1089/wound.2017.0770 [doi]
AB  - Objective: The bacterial composition and distribution were evaluated in acute 
      standardized epidermal wounds and uninjured skin by a molecular in situ 
      technology benchmarked to conventional culturing. This was done to reveal whether 
      bacterial biofilm is present in acute wounds. Approach: On the buttock of 26 
      healthy volunteers, 28 suction blisters were made and de-roofed. Four wounds were 
      biopsied immediately after wounding, whereas the remaining 24 wounds were treated 
      daily with sterile deionized water and covered with a moisture-retaining 
      dressing. On day 4 post-wounding, swabs were obtained for culturing from the 
      wounds and adjacent skin, and the wounds including adjacent skin were excised. 
      Tissue sections were stained with peptide nucleic acid (PNA) fluorescence in situ 
      hybridization (FISH) probes, counterstained by 4',6-diamidino-2-phenylindole, and 
      evaluated by confocal laser scanning microscopy (CLSM). Results: No bacterial 
      aggregates were detected at day 0. At day 4, coagulase-negative staphylococci 
      (CoNS) were the sole bacteria identified by CLSM/PNA-FISH and culturing. CoNS was 
      isolated from 78% of the wound swabs and 48% of the skin swabs. Bacterial 
      aggregates (5-150 mum) were detected by PNA-FISH/CLSM in the split stratum corneum 
      and fibrin deposits at the wound edges and in the stratum corneum and the hair 
      follicles of the adjacent skin. The bacterial aggregates were more common 
      (p = 0.0084) and larger (p = 0.0083) at wound edges than in the adjacent skin. 
      Innovation: Bacterial aggregates can establish in all wound types and may have 
      clinical significance in acute wounds. Conclusion: Bacterial aggregates were 
      observed at the edges of acute epidermal wounds, indicating initiated 
      establishment of a biofilm.
FAU - Bay, Lene
AU  - Bay L
AD  - Costerton Biofilm Center, Department of Immunology and Microbiology, University 
      of Copenhagen, Copenhagen, Denmark.
FAU - Kragh, Kasper N
AU  - Kragh KN
AD  - Costerton Biofilm Center, Department of Immunology and Microbiology, University 
      of Copenhagen, Copenhagen, Denmark.
FAU - Eickhardt, Steffen R
AU  - Eickhardt SR
AD  - Costerton Biofilm Center, Department of Immunology and Microbiology, University 
      of Copenhagen, Copenhagen, Denmark.
FAU - Poulsen, Steen S
AU  - Poulsen SS
AD  - Endocrinology, Department of Biomedical Science, University of Copenhagen, 
      Copenhagen, Denmark.
FAU - Gjerdrum, Lise Mette R
AU  - Gjerdrum LMR
AD  - Department of Pathology, Zealand University Hospital, Roskilde, Denmark.
FAU - Ghathian, Khaled
AU  - Ghathian K
AD  - Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark.
FAU - Calum, Henrik
AU  - Calum H
AD  - Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark.
FAU - Agren, Magnus S
AU  - Agren MS
AD  - Copenhagen Wound Healing Center and Digestive Disease Center, Bispebjerg 
      Hospital, University of Copenhagen, Copenhagen, Denmark.
FAU - Bjarnsholt, Thomas
AU  - Bjarnsholt T
AD  - Costerton Biofilm Center, Department of Immunology and Microbiology, University 
      of Copenhagen, Copenhagen, Denmark.
AD  - Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Adv Wound Care (New Rochelle)
JT  - Advances in wound care
JID - 101590593
PMC - PMC5905854
OTO - NOTNLM
OT  - PNA-FISH
OT  - acute wounds
OT  - bacterial aggregates
OT  - biofilm
OT  - confocal microscopy
OT  - standardized epidermal wounds
EDAT- 2018/04/21 06:00
MHDA- 2018/04/21 06:01
PMCR- 2018/04/01
CRDT- 2018/04/21 06:00
PHST- 2017/10/23 00:00 [received]
PHST- 2017/12/09 00:00 [accepted]
PHST- 2018/04/21 06:00 [entrez]
PHST- 2018/04/21 06:00 [pubmed]
PHST- 2018/04/21 06:01 [medline]
PHST- 2018/04/01 00:00 [pmc-release]
AID - 10.1089/wound.2017.0770 [pii]
AID - 10.1089/wound.2017.0770 [doi]
PST - ppublish
SO  - Adv Wound Care (New Rochelle). 2018 Apr 1;7(4):105-113. doi: 
      10.1089/wound.2017.0770.