PMID- 29675371
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2222-3959 (Print)
IS  - 2227-4898 (Electronic)
IS  - 2222-3959 (Linking)
VI  - 11
IP  - 4
DP  - 2018
TI  - Mesenchymal stem cells-derived exosomes ameliorate blue light stimulation in 
      retinal pigment epithelium cells and retinal laser injury by VEGF-dependent 
      mechanism.
PG  - 559-566
LID - 10.18240/ijo.2018.04.04 [doi]
AB  - AIM: To observe the effect of exosomes derived from human umbilical cord blood 
      mesenchymal stem cells (hUCMSCs) on the expression of vascular endothelial growth 
      factor-A (VEGF-A) in blue light injured human retinal pigment epithelial (RPE) 
      cells and laser-induced choroidal neovascularization (CNV) in rats. METHODS: 
      Exosomes were isolated from hUCMSCs and characterized by transmission electron 
      microscope and Western blot. MSCs-derived exosomes were cultured with RPE cells 
      exposed to blue light. The mRNA and protein expression of VEGF-A were determined 
      by real time-polymerase chain reaction (PCR) and Western blot, respectively. 
      Immunofluorescence assay was used for the detection of the expression level of 
      VEGF-A. We injected different doses of MSCs-derived exosomes intravitreally to 
      observe and compare their effects in a mouse model of laser-induced retinal 
      injury. The histological structure of CNV in rats was inspected by 
      hematoxylin-eosin (HE) staining and fundus fluorescein angiography. The 
      expression of VEGF-A was detected by immunohistochemistry. RESULTS: Exosomes 
      exhibited the typical characteristic morphology (cup-shaped) and size (diameter 
      between 50 and 150 nm). The exosomes marker, CD63, and hUCMSCs marker, CD90, 
      showed a robust presence. In vitro, MSCs-derived exosomes downregulated the 
      mRNA(Exo-L: t=6.485, 7.959, 9.286; Exo-M: t=7.517, 10.170, 13.413; Exo-H: 
      t=10.317, 12.234, 14.592, P<0.05) and protein (Exo-L: t=2.945, 4.477, 6.657; 
      Exo-M: t=4.713, 6.421, 8.836; Exo-H: t=6.539, 12.194, 12.783; P<0.05) expression 
      of VEGF-A in RPE cells after blue light stimulation. In vivo, we found that the 
      MSCs-derived exosomes reduced damage, distinctly downregulated VEGF-A (Exo-H: 
      t=0.957, 1.382; P<0.05), and gradually improved the histological structures of 
      CNV for a better visual function (Exo-L: 0.346, Exo-M: 3.382, Exo-H: 8.571; 
      P<0.05). CONCLUSION: MSCs-derived exosomes ameliorate blue light stimulation in 
      RPE cells and laser-induced retinal injury via downregulation of VEGF-A.
FAU - He, Guang-Hui
AU  - He GH
AD  - Tianjin Eye Hospital; Tianjin Key Lab of Ophthalmology and Visual Science; 
      Tianjin Eye Institute; Clinical College of Ophthalmology, Tianjin Medical 
      University, Tianjin 300020, China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Tianjin Eye Hospital; Tianjin Key Lab of Ophthalmology and Visual Science; 
      Tianjin Eye Institute; Clinical College of Ophthalmology, Tianjin Medical 
      University, Tianjin 300020, China.
FAU - Ma, Ying-Xue
AU  - Ma YX
AD  - Tianjin Eye Hospital; Tianjin Key Lab of Ophthalmology and Visual Science; 
      Tianjin Eye Institute; Clinical College of Ophthalmology, Tianjin Medical 
      University, Tianjin 300020, China.
FAU - Yang, Jing
AU  - Yang J
AD  - Tianjin Eye Hospital; Tianjin Key Lab of Ophthalmology and Visual Science; 
      Tianjin Eye Institute; Clinical College of Ophthalmology, Tianjin Medical 
      University, Tianjin 300020, China.
FAU - Chen, Li
AU  - Chen L
AD  - Tianjin Eye Hospital; Tianjin Key Lab of Ophthalmology and Visual Science; 
      Tianjin Eye Institute; Clinical College of Ophthalmology, Tianjin Medical 
      University, Tianjin 300020, China.
FAU - Song, Jian
AU  - Song J
AD  - Tianjin Eye Hospital; Tianjin Key Lab of Ophthalmology and Visual Science; 
      Tianjin Eye Institute; Clinical College of Ophthalmology, Tianjin Medical 
      University, Tianjin 300020, China.
FAU - Chen, Song
AU  - Chen S
AD  - Tianjin Eye Hospital; Tianjin Key Lab of Ophthalmology and Visual Science; 
      Tianjin Eye Institute; Clinical College of Ophthalmology, Tianjin Medical 
      University, Tianjin 300020, China.
LA  - eng
PT  - Journal Article
DEP - 20180418
PL  - China
TA  - Int J Ophthalmol
JT  - International journal of ophthalmology
JID - 101553860
PMC - PMC5902357
OTO - NOTNLM
OT  - choroidal neovascularization
OT  - exosome
OT  - human umbilical cord mesenchymal stem cell
OT  - retinal pigment epithelial cell
OT  - vascular endothelial growth factor
EDAT- 2018/04/21 06:00
MHDA- 2018/04/21 06:01
PMCR- 2018/04/18
CRDT- 2018/04/21 06:00
PHST- 2017/08/04 00:00 [received]
PHST- 2018/02/07 00:00 [accepted]
PHST- 2018/04/21 06:00 [entrez]
PHST- 2018/04/21 06:00 [pubmed]
PHST- 2018/04/21 06:01 [medline]
PHST- 2018/04/18 00:00 [pmc-release]
AID - ijo-11-04-559 [pii]
AID - 10.18240/ijo.2018.04.04 [doi]
PST - epublish
SO  - Int J Ophthalmol. 2018 Apr 18;11(4):559-566. doi: 10.18240/ijo.2018.04.04. 
      eCollection 2018.