PMID- 29676074
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2092-7355 (Print)
IS  - 2092-7363 (Electronic)
IS  - 2092-7355 (Linking)
VI  - 10
IP  - 3
DP  - 2018 May
TI  - Immune Characterization of Bone Marrow-Derived Models of Mucosal and Connective 
      Tissue Mast Cells.
PG  - 268-277
LID - 10.4168/aair.2018.10.3.268 [doi]
AB  - PURPOSE: It is well appreciated that mast cells (MCs) demonstrate tissue-specific 
      imprinting, with different biochemical and functional properties between 
      connective tissue MCs (CTMCs) and mucosal MCs (MMCs). Although in vitro systems 
      have been developed to model these different subsets, there has been limited 
      investigation into the functional characteristics of the 2 major MC subsets. 
      Here, we report the immunologic characterization of 2 MCs subsets developed in 
      vitro from bone marrow progenitors modeling MMCs and CTMCs. METHODS: We grew bone 
      marrow for 4 weeks in the presence of transforming growth factor (TGF)-beta, 
      interleukin (IL)-9, IL-3, and stem cell factor (SCF) to generate MMCs, and IL-4, 
      IL-3, and SCF to generate CTMCs. RESULTS: CTMCs and MMCs differed in growth rate 
      and protease content, but their immune characteristics were remarkably similar. 
      Both subsets responded to immunoglobulin E (IgE)-mediated activation with 
      signaling, degranulation, and inflammatory cytokine release, although differences 
      between subsets were noted in IL-10. CTMCs and MMCs showed a similar toll-like 
      receptor (TLR) expression profile, dominated by expression of TLR4, TLR6, or both 
      subsets were responsive to lipopolysaccharide (LPS), but not poly(I:C). CTMCs and 
      MMCs express receptors for IL-33 and thymic stromal lymphopoietin (TSLP), and 
      respond to these cytokines alone or with modified activation in response to IgE 
      cross-linking. CONCLUSIONS: The results of this paper show the immunologic 
      characterization of bone marrow-derived MMCs and CTMCs, providing useful 
      protocols for in vitro modeling of MC subsets.
CI  - Copyright (c) 2018 The Korean Academy of Asthma, Allergy and Clinical Immunology . 
      The Korean Academy of Pediatric Allergy and Respiratory Disease.
FAU - Benede, Sara
AU  - Benede S
AUID- ORCID: 0000-0002-9288-9438
AD  - Department of Pediatrics, Mindich Child Health and Development Institute, 
      Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. 
      sbenede@ucm.es.
FAU - Cody, Evan
AU  - Cody E
AUID- ORCID: 0000-0003-0445-1119
AD  - Department of Pediatrics, Mindich Child Health and Development Institute, 
      Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
FAU - Agashe, Charuta
AU  - Agashe C
AUID- ORCID: 0000-0001-8820-8655
AD  - Department of Pediatrics, Mindich Child Health and Development Institute, 
      Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
FAU - Berin, M Cecilia
AU  - Berin MC
AUID- ORCID: 0000-0002-9051-9249
AD  - Department of Pediatrics, Mindich Child Health and Development Institute, 
      Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Allergy Asthma Immunol Res
JT  - Allergy, asthma & immunology research
JID - 101518382
PMC - PMC5911446
OTO - NOTNLM
OT  - Mucosal mast cells
OT  - connective tissue mast cells
OT  - mast cell subsets
COIS- There are no financial or other issues that might lead to conflict of interest.
EDAT- 2018/04/21 06:00
MHDA- 2018/04/21 06:01
PMCR- 2018/05/01
CRDT- 2018/04/21 06:00
PHST- 2017/08/18 00:00 [received]
PHST- 2017/12/08 00:00 [revised]
PHST- 2017/12/12 00:00 [accepted]
PHST- 2018/04/21 06:00 [entrez]
PHST- 2018/04/21 06:00 [pubmed]
PHST- 2018/04/21 06:01 [medline]
PHST- 2018/05/01 00:00 [pmc-release]
AID - 10.268 [pii]
AID - 10.4168/aair.2018.10.3.268 [doi]
PST - ppublish
SO  - Allergy Asthma Immunol Res. 2018 May;10(3):268-277. doi: 
      10.4168/aair.2018.10.3.268.