PMID- 29677430 OWN - NLM STAT- MEDLINE DCOM- 20180516 LR - 20180816 IS - 0033-2240 (Print) IS - 0033-2240 (Linking) VI - 73 IP - 8 DP - 2016 TI - Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) blood levels in patients with acute carbon monoxide poisoning - a preliminary observations. PG - 552-9 AB - BACKGROUND: Neurotrophins are the family of proteins which stimulate and regulate the process of neurogenesis. Several factors belong to the family, mainly nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT 3), and neurotrophin-4/5 (NT-4/5). Acute poisoning with carbon monoxide (CO), which usually is accompanied by neurologic symptoms, can potentially change the secretion profile of neurotrophins. Aim of the study. The main goal of the study is to assess the changes of NGF and BDNF plasma levels during an acute phase of CO poisoning as well as immediately after recovery. Additionally, the relationship among neurotrophin levels and selected aspects of clinical course of CO poisoning were studied. MATERIALS AND METHODS: The study group consisted of 18 patients (mean age: 31.8+/-10.3 years) hospitalized in Toxicology Department of University Hospital in Cracow because of acute CO poisoning. There were 10 women (mean age: 30.2+/-6.9 years) and 8 men (mean age 33.9+/-13.7 years) in the group. The levels of NGF and BDNF were evaluated using immunoenzymatic method (ELISA) in plasma samples taken thrice in each patient. The sample 1. was taken during hospital admission, the sample 2. about 12-36 hours after admission, and the sample 3. just before the hospital discharging (usually, on the 3rd-4th day). The clinical data were collected from patients' anamnesis, physical examination and neuropsychological evaluation. The statistical analysis were performed using tools comprised in STATISTICA 12.0 PL (StatSoft Polska, Cracow, Poland) software. RESULTS: The majority of NGF plasma levels were less than 14 pg/mL (values below the limit of quantification), contrary to the sole case of 34.3 pg/mL. BDNF plasma levels ranged from 4.8 ng/mL to above 48 ng/mL, i.e. they were higher than the upper limit of measurement range for the plasma dilution which had been used. The comparison of NGF and BDNF plasma levels in the study group with their analogues in healthy volunteers taken from the literature indicates that NGF level declines and BDNF level rises in patients with CO poisoning. The profile of BDNF concentrations in the majority of patients formed the characteristic pattern: BDNF sample 1. > BDNF sample 2. < BDNF sample 3. Taking all the values of BDNF higher than 48 ng/mL as equal to 48 ng/ mL, the statistically significant difference among 3 sample series was found according to BDNF levels. Maintaining the above mentioned assumption, the statistically significant negative correlation between the number of higher cognitive functions disturbed in one patient at the same time and the BDNF levels in sample series 2 was discovered, as well as the weak correlations between BDNF level in sample series 1 and carboxyhaemoglobin or lactate level. Moreover, weak but statistically significant correlations were present between the duration of CO exposure and BDNF levels in each sample series. CONCLUSIONS: The NGF plasma level is probably declined, while the BDNF plasma level is increased in patients with acute CO poisoning. The concentration-time curve for the plasma BDNF may sometimes undergo fluctuations with two peaks on its course. Plasma BDNF level may serve as a biological marker of disturbed higher cognitive functions in acute CO poisoning. Some clinical aspects of CO poisoning (duration of exposure, HbCO and lactate blood levels) may influence BDNF level. FAU - Ciszowski, Krzysztof AU - Ciszowski K FAU - Gomolka, Ewa AU - Gomolka E FAU - Gawlikowski, Tomasz AU - Gawlikowski T FAU - Szpak, Dorota AU - Szpak D FAU - Potoczek, Anna AU - Potoczek A FAU - Boba, Magdalena AU - Boba M LA - eng PT - Journal Article PL - Poland TA - Przegl Lek JT - Przeglad lekarski JID - 19840720R RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (NGF protein, human) RN - 7171WSG8A2 (BDNF protein, human) RN - 9061-61-4 (Nerve Growth Factor) SB - IM MH - Adult MH - Brain-Derived Neurotrophic Factor/*blood MH - Carbon Monoxide Poisoning/*blood MH - Enzyme-Linked Immunosorbent Assay MH - Female MH - Humans MH - Male MH - Middle Aged MH - Nerve Growth Factor/*blood MH - Young Adult EDAT- 2016/01/01 00:00 MHDA- 2018/05/17 06:00 CRDT- 2018/04/21 06:00 PHST- 2018/04/21 06:00 [entrez] PHST- 2016/01/01 00:00 [pubmed] PHST- 2018/05/17 06:00 [medline] PST - ppublish SO - Przegl Lek. 2016;73(8):552-9.