PMID- 29679285
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1752-153X (Print)
IS  - 1752-153X (Electronic)
IS  - 1752-153X (Linking)
VI  - 12
IP  - 1
DP  - 2018 Apr 20
TI  - Exploration of Phyllanthus acidus mediated silver nanoparticles and its activity 
      against infectious bacterial pathogen.
PG  - 42
LID - 10.1186/s13065-018-0412-7 [doi]
LID - 42
AB  - In our present investigation, synthesis of nontoxic, eco friendly and cost 
      effective silver nanoparticles, Phyllanthus acidus (P. acidus) was used as 
      starting material. The influence of phyto-constituents present in aqueous 
      extracts of Phyllanthus acidus was found to be effective in reduction of silver 
      nitrate to free silver nanoparticles (PA-AgNPs). HPTLC finger print analysis 
      reveals the presence of flavonoid, quercetin in aqueous extracts of Phyllanthus 
      acidus. Surface plasmon racemonance exhibited lambda max at 462 nm through UV-Vis 
      spectroscopy. Zeta size revealed that the size of nanoparticles were with in the 
      range of 65-250 nm with polydisperse index (PDI) of 0.451. The negative charge of 
      zeta potential value (- 16.4) indicates repulsion among PA-AgNPs with their 
      excellent stability. FESEM-EDAX, XRD and TEM analysis confirmed the presence of 
      nano-crystalline PA-AgNPs with different morphological textures. Further, 
      PA-AgNPs has shown potent antibacterial effect on E. coli cells. The greater 
      antibacterial effect (viable and dead cells) of PA-AgNPs were confirmed by using 
      acridine orange (AO) dye which can able to provide insight of healthy as well as 
      damaged DNA. Live cells emit florescence green and dead cells (treated with 
      PA-AgNPS at 20 and 40 microg/ml) appear as pale orange red colour. Post treatment, 
      investigations of PA-AgNPs on E. coli cells under SEM was found to be effective 
      against cell membrane damages which leads to cell death or cell growth arrest. 
      Hence, from the above findings, we strongly recommend silver nanoparticles from 
      Phyllanthus acidus can be used as a potential source for antimicrobial agent for 
      chronic infections and also against other harmful microorganisms.
FAU - Sowmya, Cherukuri
AU  - Sowmya C
AD  - Drug Delivery and Nanotechnology Laboratory (DDNL), Department of Pharmaceutics, 
      Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil, 
      Virudhunagar Dist, Srivilliputtur, 626126, Tamil Nadu, India.
FAU - Lavakumar, Vuppalapati
AU  - Lavakumar V
AD  - Drug Delivery and Nanotechnology Laboratory (DDNL), Department of Pharmaceutics, 
      Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil, 
      Virudhunagar Dist, Srivilliputtur, 626126, Tamil Nadu, India. 
      lavanyalavakumar@gmail.com.
FAU - Venkateshan, Narayanan
AU  - Venkateshan N
AD  - Drug Delivery and Nanotechnology Laboratory (DDNL), Department of Pharmaceutics, 
      Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil, 
      Virudhunagar Dist, Srivilliputtur, 626126, Tamil Nadu, India.
FAU - Ravichandiran, Velayutham
AU  - Ravichandiran V
AD  - National Institute of Pharamceutical Education and Research, NIPER - Kolkata at 
      Indian Institute of Chemical Biology, Raja S. C. Mullick Road, Jadavpur, 700032, 
      Kolkata, India.
FAU - Saigopal, D V R
AU  - Saigopal DVR
AD  - Department of Virology, Sri Venkateswara University, Tirupati, 517570, Andhra 
      Pradesh, India.
LA  - eng
PT  - Journal Article
DEP - 20180420
PL  - England
TA  - Chem Cent J
JT  - Chemistry Central journal
JID - 101314213
PMC - PMC5910440
OTO - NOTNLM
OT  - Antibacterial activity
OT  - Cell surface morphology studies
OT  - Fluorescence microscopy studies
OT  - Phyllanthus acidus
OT  - Silver nanoparticles
EDAT- 2018/04/22 06:00
MHDA- 2018/04/22 06:01
PMCR- 2018/04/20
CRDT- 2018/04/22 06:00
PHST- 2017/09/29 00:00 [received]
PHST- 2018/04/13 00:00 [accepted]
PHST- 2018/04/22 06:00 [entrez]
PHST- 2018/04/22 06:00 [pubmed]
PHST- 2018/04/22 06:01 [medline]
PHST- 2018/04/20 00:00 [pmc-release]
AID - 10.1186/s13065-018-0412-7 [pii]
AID - 412 [pii]
AID - 10.1186/s13065-018-0412-7 [doi]
PST - epublish
SO  - Chem Cent J. 2018 Apr 20;12(1):42. doi: 10.1186/s13065-018-0412-7.