PMID- 29679543
OWN - NLM
STAT- MEDLINE
DCOM- 20190506
LR  - 20200930
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1691
DP  - 2018 Jul 15
TI  - Retrograde influences of SCG axotomy on uninjured preganglionic neurons.
PG  - 44-54
LID - S0006-8993(18)30202-6 [pii]
LID - 10.1016/j.brainres.2018.04.014 [doi]
AB  - There is evidence that neuronal injury can affect uninjured neurons in the same 
      neural circuit. The overall goal of this study was to understand the effects of 
      peripheral nerve injury on uninjured neurons located in the central nervous 
      system (CNS). As a model, we examined whether axotomy (transection of 
      postganglionic axons) of the superior cervical ganglion (SCG) affected the 
      uninjured, preganglionic neurons that innervate the SCG. At 7 days post-injury a 
      reduction in choline acetyltransferase (ChAT) and synaptophysin immunoreactivity 
      in the SCG, both markers for preganglionic axons, was observed, and this 
      reduction persisted at 8 and 12 weeks post-injury. No changes were observed in 
      the number or size of the parent cell bodies in the intermediolateral cell column 
      (IML) of the spinal cord, yet synaptic input to the IML neurons was decreased at 
      both 8 and 12 weeks post-injury. In order to understand the mechanisms underlying 
      these changes, protein levels of brain-derived neurotrophic factor (BDNF) and 
      tyrosine receptor kinase B (TrkB) were examined and reductions were observed at 
      7 days post-injury in both the SCG and spinal cord. Taken together these results 
      suggest that axotomy of the SCG led to reduced BDNF in the SCG and spinal cord, 
      which in turn influenced ChAT and synaptophysin expression in the SCG and also 
      contributed to the altered synaptic input to the IML neurons. More generally 
      these findings provide evidence that the effects of peripheral injury can cascade 
      into the CNS and affect uninjured neurons.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Gannon, Sean M
AU  - Gannon SM
AD  - Center for Neuroscience and Behavior, Miami University, Oxford, OH 45056, United 
      States; Department of Biology, Miami University, Oxford, OH 45056, United States.
FAU - Hawk, Kiel
AU  - Hawk K
AD  - Center for Neuroscience and Behavior, Miami University, Oxford, OH 45056, United 
      States; Graduate Program in Cell, Molecular, and Structural Biology, Miami 
      University, Oxford, OH 45056, United States.
FAU - Walsh, Brian F
AU  - Walsh BF
AD  - Department of Biology, Miami University, Oxford, OH 45056, United States.
FAU - Coulibaly, Aminata
AU  - Coulibaly A
AD  - Center for Neuroscience and Behavior, Miami University, Oxford, OH 45056, United 
      States; Graduate Program in Cell, Molecular, and Structural Biology, Miami 
      University, Oxford, OH 45056, United States.
FAU - Isaacson, Lori G
AU  - Isaacson LG
AD  - Center for Neuroscience and Behavior, Miami University, Oxford, OH 45056, United 
      States; Graduate Program in Cell, Molecular, and Structural Biology, Miami 
      University, Oxford, OH 45056, United States; Department of Biology, Miami 
      University, Oxford, OH 45056, United States. Electronic address: 
      isaacslg@miamioh.edu.
LA  - eng
GR  - R15 NS051206/NS/NINDS NIH HHS/United States
GR  - R15 NS095314/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20180419
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Ntrk2 protein, rat)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Autonomic Nervous System Diseases/etiology/*pathology
MH  - Autonomic Pathways
MH  - Axotomy
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Functional Laterality/*physiology
MH  - Gene Expression Regulation/physiology
MH  - Neurons/metabolism/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/metabolism
MH  - Superior Cervical Ganglion/*injuries/*pathology
MH  - Time Factors
PMC - PMC6082408
MID - NIHMS963562
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor (BDNF)
OT  - Choline acetyltransferase (ChAT)
OT  - Full length TrkB receptor (TrkB.FL)
OT  - Intermediolateral cell column (IML)
OT  - Spinal cord
OT  - Synaptophysin
OT  - Truncated TrkB receptor (TrkB.T1)
EDAT- 2018/04/22 06:00
MHDA- 2019/05/07 06:00
PMCR- 2019/07/15
CRDT- 2018/04/22 06:00
PHST- 2017/10/06 00:00 [received]
PHST- 2018/04/06 00:00 [revised]
PHST- 2018/04/13 00:00 [accepted]
PHST- 2018/04/22 06:00 [pubmed]
PHST- 2019/05/07 06:00 [medline]
PHST- 2018/04/22 06:00 [entrez]
PHST- 2019/07/15 00:00 [pmc-release]
AID - S0006-8993(18)30202-6 [pii]
AID - 10.1016/j.brainres.2018.04.014 [doi]
PST - ppublish
SO  - Brain Res. 2018 Jul 15;1691:44-54. doi: 10.1016/j.brainres.2018.04.014. Epub 2018 
      Apr 19.