PMID- 29680389
OWN - NLM
STAT- MEDLINE
DCOM- 20181001
LR  - 20181001
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 234
DP  - 2018 Apr
TI  - Testing of umbilical cords by real time PCR is suitable for assessing vertical 
      transmission of porcine reproductive and respiratory syndrome virus under field 
      conditions.
PG  - 27-29
LID - S1090-0233(18)30008-X [pii]
LID - 10.1016/j.tvjl.2018.01.008 [doi]
AB  - The objective of this study was to test the suitability of umbilical cord (UC) 
      sampling and ear vein swabbing (EVS) as alternatives to jugular vein bleeding 
      (JVB) for the assessment of vertical transmission of porcine reproductive and 
      respiratory syndrome virus (PRRSV). Twelve farms suspected to be PRRSV-positive 
      unstable were selected and the three types of samples were obtained from 21 
      batches of newborn piglets (n=387). The proportions of positive results, viral 
      loads and time spent to collect the samples were compared. UC yielded the highest 
      detection rate, with 76 positives compared to 55 JVB- and 45 EV-positive results 
      (P<0.05). Average Ct values were 26.6+/-8.5 for JVB, 30.8+/-6.4 for EV and 32.1+/-4.85 
      for UC (P<0.01). UC was the fastest collection method (mean 24s vs. 55s for EV 
      and 72s for JVB; P<0.05). In this study, UC testing was a faster and more 
      sensitive alternative to JVB or EV for the detection of PRRSV in newborn piglets.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Martin-Valls, Gerard E
AU  - Martin-Valls GE
AD  - Department Sanitat i Anatomia Animals, Facultat de Veterinaria, Universitat 
      Autonoma de Barcelona, 08193 Cerdanyola del Valles, Spain; Centre de Recerca en 
      Sanitat Animal (CReSA-IRTA-UAB), Edifici CRESA, Campus UAB, 08193 Cerdanyola del 
      Valles, Spain. Electronic address: gerard.martin@uab.cat.
FAU - Hidalgo, Mireia
AU  - Hidalgo M
AD  - Centre de Recerca en Sanitat Animal (CReSA-IRTA-UAB), Edifici CRESA, Campus UAB, 
      08193 Cerdanyola del Valles, Spain.
FAU - Cano, Esmeralda
AU  - Cano E
AD  - Centre de Recerca en Sanitat Animal (CReSA-IRTA-UAB), Edifici CRESA, Campus UAB, 
      08193 Cerdanyola del Valles, Spain.
FAU - Mateu, Enric
AU  - Mateu E
AD  - Department Sanitat i Anatomia Animals, Facultat de Veterinaria, Universitat 
      Autonoma de Barcelona, 08193 Cerdanyola del Valles, Spain; Centre de Recerca en 
      Sanitat Animal (CReSA-IRTA-UAB), Edifici CRESA, Campus UAB, 08193 Cerdanyola del 
      Valles, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180201
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
SB  - IM
MH  - Animals
MH  - Female
MH  - Infectious Disease Transmission, Vertical/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*transmission
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Pregnancy
MH  - Real-Time Polymerase Chain Reaction/*veterinary
MH  - Swine
MH  - Umbilical Cord/*virology
OTO - NOTNLM
OT  - Diagnosis
OT  - Monitoring
OT  - PRRSV
OT  - Vertical transmission
EDAT- 2018/04/24 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/04/23 06:00
PHST- 2017/07/24 00:00 [received]
PHST- 2018/01/25 00:00 [revised]
PHST- 2018/01/28 00:00 [accepted]
PHST- 2018/04/23 06:00 [entrez]
PHST- 2018/04/24 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
AID - S1090-0233(18)30008-X [pii]
AID - 10.1016/j.tvjl.2018.01.008 [doi]
PST - ppublish
SO  - Vet J. 2018 Apr;234:27-29. doi: 10.1016/j.tvjl.2018.01.008. Epub 2018 Feb 1.