PMID- 29681910
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 9
DP  - 2018
TI  - Specific Fluorescence in Situ Hybridization (FISH) Test to Highlight Colonization 
      of Xylem Vessels by Xylella fastidiosa in Naturally Infected Olive Trees (Olea 
      europaea L.).
PG  - 431
LID - 10.3389/fpls.2018.00431 [doi]
LID - 431
AB  - The colonization behavior of the Xylella fastidiosa strain CoDiRO, the causal 
      agent of olive quick decline syndrome (OQDS), within the xylem of Olea europaea 
      L. is still quite controversial. As previous literature suggests, even if xylem 
      vessel occlusions in naturally infected olive plants were observed, cell 
      aggregation in the formation of occlusions had a minimal role. This observation 
      left some open questions about the whole behavior of the CoDiRO strain and its 
      actual role in OQDS pathogenesis. In order to evaluate the extent of bacterial 
      infection in olive trees and the role of bacterial aggregates in vessel 
      occlusions, we tested a specific fluorescence in situ hybridization (FISH) probe 
      (KO 210) for X. fastidiosa and quantified the level of infection and vessel 
      occlusion in both petioles and branches of naturally infected and non-infected 
      olive trees. All symptomatic petioles showed colonization by X. fastidiosa, 
      especially in the larger innermost vessels. In several cases, the vessels 
      appeared completely occluded by a biofilm containing bacterial cells and 
      extracellular matrix and the frequent colonization of adjacent vessels suggested 
      a horizontal movement of the bacteria. Infected symptomatic trees had 21.6 +/- 
      10.7% of petiole vessels colonized by the pathogen, indicating an irregular 
      distribution in olive tree xylem. Thus, our observations point out the primary 
      role of the pathogen in olive vessel occlusions. Furthermore, our findings 
      indicate that the KO 210 FISH probe is suitable for the specific detection of X. 
      fastidiosa.
FAU - Cardinale, Massimiliano
AU  - Cardinale M
AD  - Institute of Applied Microbiology, Research Center for BioSystems, Land Use, and 
      Nutrition, Justus-Liebig-University Giessen, Giessen, Germany.
FAU - Luvisi, Andrea
AU  - Luvisi A
AD  - Department of Biological and Environmental Sciences and Technologies, University 
      of Salento, Lecce, Italy.
FAU - Meyer, Joana B
AU  - Meyer JB
AD  - WSL Swiss Federal Research Institute, Birmensdorf, Switzerland.
FAU - Sabella, Erika
AU  - Sabella E
AD  - Department of Biological and Environmental Sciences and Technologies, University 
      of Salento, Lecce, Italy.
FAU - De Bellis, Luigi
AU  - De Bellis L
AD  - Department of Biological and Environmental Sciences and Technologies, University 
      of Salento, Lecce, Italy.
FAU - Cruz, Albert C
AU  - Cruz AC
AD  - Department of Computer and Electrical Engineering and Computer Science, 
      California State University, Bakersfield, CA, United States.
FAU - Ampatzidis, Yiannis
AU  - Ampatzidis Y
AD  - Department of Agricultural and Biological Engineering, Southwest Florida Research 
      and Education Center, University of Florida, Gainesville, FL, United States.
FAU - Cherubini, Paolo
AU  - Cherubini P
AD  - WSL Swiss Federal Research Institute, Birmensdorf, Switzerland.
LA  - eng
PT  - Journal Article
DEP - 20180406
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC5897508
OTO - NOTNLM
OT  - fluorescence in situ hybridization-confocal laser scanning microscopy (FISH-CLSM)
OT  - olive quick decline syndrome (OQDS)
OT  - phytopathogen
OT  - vessel occlusion
OT  - xylem
EDAT- 2018/04/24 06:00
MHDA- 2018/04/24 06:01
PMCR- 2018/01/01
CRDT- 2018/04/24 06:00
PHST- 2018/01/07 00:00 [received]
PHST- 2018/03/21 00:00 [accepted]
PHST- 2018/04/24 06:00 [entrez]
PHST- 2018/04/24 06:00 [pubmed]
PHST- 2018/04/24 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2018.00431 [doi]
PST - epublish
SO  - Front Plant Sci. 2018 Apr 6;9:431. doi: 10.3389/fpls.2018.00431. eCollection 
      2018.