PMID- 29683472
OWN - NLM
STAT- MEDLINE
DCOM- 20190405
LR  - 20190405
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 592
IP  - 10
DP  - 2018 May
TI  - Proteomic profiling of Ganoderma tsugae ethanol extract-induced adipogenesis 
      displaying browning features.
PG  - 1643-1666
LID - 10.1002/1873-3468.13061 [doi]
AB  - Ganoderma is classified as a top grade traditional Chinese medicine for promoting 
      human health by regulating 'vital energy'. Its potency towards metabolism and 
      energy homeostasis, particularly, metabolic adaptations of adipocytes, needs to 
      be re-evaluated through an evidence-based study. Here, the triterpenoid-rich 
      Ganoderma tsugae ethanol extract (GTEE) was found to contribute towards 
      adipogenesis accompanied with elevated intracellular lipid metabolic flux. 
      Additionally, proteomic profiling revealed GTEE-upregulated mitochondrial 
      remodeling and chemical energy redox modifications, which display UCP1-positive 
      browning fat-selective features and a NADH-mediated adaptive mechanism. 
      GTEE-treated mice with diet-induced obesity also resulted in the amelioration of 
      white adipocyte hypertrophy and the appearance of UCP1-positive browning 
      adipocytes. Our novel findings unravel that GTEE could promote intracellular 
      metabolic flexibility and plasticity followed by the induction of adipocyte 
      browning.
CI  - (c) 2018 Federation of European Biochemical Societies.
FAU - Tseng, Hsiu-Hsueh
AU  - Tseng HH
AD  - Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, 
      Taiwan, ROC.
FAU - Yeh, Wan-Chun
AU  - Yeh WC
AD  - Department of Biological Science and Technology, College of Biopharmaceutical and 
      Food Sciences, China Medical University, Taichung, Taiwan, ROC.
FAU - Tu, Yun-Chen
AU  - Tu YC
AD  - Department of Biological Science and Technology, College of Biopharmaceutical and 
      Food Sciences, China Medical University, Taichung, Taiwan, ROC.
FAU - Yang, Bi-Fen
AU  - Yang BF
AD  - Department of Biological Science and Technology, College of Biopharmaceutical and 
      Food Sciences, China Medical University, Taichung, Taiwan, ROC.
FAU - Lai, Yen-Ting
AU  - Lai YT
AD  - Department of Biological Science and Technology, College of Biopharmaceutical and 
      Food Sciences, China Medical University, Taichung, Taiwan, ROC.
FAU - Lee, Hsien-Kuang
AU  - Lee HK
AD  - Graduate Institute of Basic Medical Sciences, College of Medicine, China Medical 
      University, Taichung, Taiwan, ROC.
FAU - Yang, Yo-Chang
AU  - Yang YC
AD  - Department of Biological Science and Technology, College of Biopharmaceutical and 
      Food Sciences, China Medical University, Taichung, Taiwan, ROC.
FAU - Huang, Hui-Chi
AU  - Huang HC
AD  - Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, 
      College of Chinese Medicine, China Medical University, Taichung, Taiwan, ROC.
FAU - Lee, Yi-Jen
AU  - Lee YJ
AD  - Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, 
      Taiwan, ROC.
FAU - Ou, Chien-Chih
AU  - Ou CC
AD  - Department of Pharmacology, National Defense Medical Center, Taipei, Taiwan, ROC.
FAU - Kuo, Han-Peng
AU  - Kuo HP
AD  - Research and Development Unit, Sinphar Group, I-Lan, Taiwan, ROC.
FAU - Kuo, Yueh-Hsiung
AU  - Kuo YH
AD  - Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, 
      College of Chinese Medicine, China Medical University, Taichung, Taiwan, ROC.
AD  - Tsuzuki Institute for Traditional Medicine, College of Pharmacy, China Medical 
      University, Taichung, Taiwan, ROC.
FAU - Kao, Ming-Ching
AU  - Kao MC
AD  - Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, 
      Taiwan, ROC.
AD  - Department of Biological Science and Technology, College of Biopharmaceutical and 
      Food Sciences, China Medical University, Taichung, Taiwan, ROC.
FAU - Liu, Jah-Yao
AU  - Liu JY
AD  - Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, 
      Taiwan, ROC.
AD  - Department of Obstetrics & Gynecology, Tri-Service General Hospital, National 
      Defense Medical Center, Taipei, Taiwan, ROC.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180511
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Uncoupling Protein 1)
RN  - 0U46U6E8UK (NAD)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes, Brown/metabolism
MH  - Adipocytes, White/metabolism
MH  - Adipogenesis/*drug effects
MH  - Animals
MH  - Blotting, Western
MH  - Diet
MH  - Drugs, Chinese Herbal/chemistry/*pharmacology
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Ethanol/chemistry
MH  - Fungal Proteins/*metabolism
MH  - Ganoderma/chemistry/*metabolism
MH  - Male
MH  - Mice
MH  - NAD/metabolism
MH  - Obesity/prevention & control
MH  - Organelle Biogenesis
MH  - Oxidation-Reduction
MH  - *Proteomics
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Uncoupling Protein 1/metabolism
OTO - NOTNLM
OT  - Ganoderma tsugae
OT  - UCP1
OT  - adipogenesis
OT  - lipid metabolism
OT  - metabolic adaptations
OT  - proteomics
EDAT- 2018/04/24 06:00
MHDA- 2019/04/06 06:00
CRDT- 2018/04/24 06:00
PHST- 2017/12/14 00:00 [received]
PHST- 2018/03/16 00:00 [revised]
PHST- 2018/03/31 00:00 [accepted]
PHST- 2018/04/24 06:00 [pubmed]
PHST- 2019/04/06 06:00 [medline]
PHST- 2018/04/24 06:00 [entrez]
AID - 10.1002/1873-3468.13061 [doi]
PST - ppublish
SO  - FEBS Lett. 2018 May;592(10):1643-1666. doi: 10.1002/1873-3468.13061. Epub 2018 
      May 11.