PMID- 2968368
OWN - NLM
STAT- MEDLINE
DCOM- 19880803
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 81
IP  - 6
DP  - 1988 Jun
TI  - Synthesis of atrial natriuretic polypeptide in human failing hearts. Evidence for 
      altered processing of atrial natriuretic polypeptide precursor and augmented 
      synthesis of beta-human ANP.
PG  - 1962-70
AB  - To elucidate the synthesis of atrial natriuretic polypeptide (ANP) in the failing 
      heart, 20 human right auricles obtained at cardiovascular surgery were studied. 
      The concentration of alpha-human ANP-like immunoreactivity (alpha-hANP-LI) in 
      human right auricles ranged from 13.8 to 593.5 micrograms/g, and the tissue 
      alpha-hANP-LI concentration in severe congestive heart failure (CHF) (New York 
      Heart Association [NYHA] functional class III and class IV) (235.4 +/- 57.2 
      micrograms/g) was much higher than that in mild CHF (NYHA class I and class II) 
      (52.5 +/- 15.6 micrograms/g). Atrial alpha-hANP-LI levels were significantly 
      correlated with plasma concentrations of alpha-hANP-LI in these patients (r = 
      0.84, P less than 0.01). High performance gel permeation chromatography and 
      reverse phase high performance liquid chromatography coupled with 
      radioimmunoassay for ANP revealed that the alpha-hANP-LI in the human auricle 
      consisted of three major components of ANP, gamma-human ANP (gamma-hANP), 
      beta-human ANP (beta-hANP) and alpha-human ANP (alpha-hANP). Comparing 
      percentages of gamma-hANP, beta-hANP, and alpha-hANP in alpha-hANP-LI in severe 
      CHF with those in mild CHF, the predominant component of alpha-hANP-LI was 
      gamma-hANP in mild CHF, whereas beta-hANP and/or alpha-hANP were prevailing in 
      severe CHF and, especially, beta-hANP was markedly increased in human failing 
      hearts. These results demonstrate that the total ANP concentration in the atrium 
      of the human heart is increased in severe CHF and that the increase of ANP in the 
      human failing heart is mainly due to the increase of small molecular weight forms 
      of ANP, beta-hANP, and alpha-hANP, especially beta-hANP, and indicate that the 
      processing of ANP precursor, or gamma-hANP, in the human failing heart differs 
      from that in the normal heart, suggesting that the failing heart augments 
      synthesis and secretion of ANP as one of its own compensatory responses.
FAU - Sugawara, A
AU  - Sugawara A
AD  - Department of Medicine, Kyoto University School of Medicine, Japan.
FAU - Nakao, K
AU  - Nakao K
FAU - Morii, N
AU  - Morii N
FAU - Yamada, T
AU  - Yamada T
FAU - Itoh, H
AU  - Itoh H
FAU - Shiono, S
AU  - Shiono S
FAU - Saito, Y
AU  - Saito Y
FAU - Mukoyama, M
AU  - Mukoyama M
FAU - Arai, H
AU  - Arai H
FAU - Nishimura, K
AU  - Nishimura K
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Protein Precursors)
RN  - 85637-73-6 (Atrial Natriuretic Factor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Atrial Natriuretic Factor/analysis/*biosynthesis
MH  - Child, Preschool
MH  - Chromatography, Gel
MH  - Chromatography, High Pressure Liquid
MH  - Female
MH  - Heart Atria/analysis/metabolism
MH  - Heart Failure/*metabolism
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Myocardium/analysis/*metabolism
MH  - Protein Precursors/*metabolism
MH  - Radioimmunoassay
PMC - PMC442649
EDAT- 1988/06/01 00:00
MHDA- 1988/06/01 00:01
PMCR- 1988/06/01
CRDT- 1988/06/01 00:00
PHST- 1988/06/01 00:00 [pubmed]
PHST- 1988/06/01 00:01 [medline]
PHST- 1988/06/01 00:00 [entrez]
PHST- 1988/06/01 00:00 [pmc-release]
AID - 10.1172/JCI113544 [doi]
PST - ppublish
SO  - J Clin Invest. 1988 Jun;81(6):1962-70. doi: 10.1172/JCI113544.