PMID- 29684808
OWN - NLM
STAT- MEDLINE
DCOM- 20181121
LR  - 20190501
IS  - 1432-0436 (Electronic)
IS  - 0301-4681 (Print)
IS  - 0301-4681 (Linking)
VI  - 101
DP  - 2018 May-Jun
TI  - Tissue interactions and estrogenic response during human female fetal 
      reproductive tract development.
PG  - 39-45
LID - S0301-4681(18)30034-3 [pii]
LID - 10.1016/j.diff.2018.04.002 [doi]
AB  - The role of tissue interactions was explored to determine whether epithelial 
      differentiation within the developing human reproductive tract is induced and 
      specified by mesenchyme in tissue recombinants composed of mouse vaginal 
      mesenchyme + human uterine tubal epithelium (mVgM+hTubE). The tissue recombinants 
      were grown in DES-treated ovariectomized athymic mice. After 2-4 weeks of in vivo 
      growth, several vaginal specific features were expressed in the human tubal 
      epithelium. The mesenchyme-induced effects included morphological change as well 
      as expression of several immunohistochemical markers. Although the 
      mesenchyme-induced shift in vaginal differentiation in the human tubal epithelium 
      was not complete, the partial induction of vaginal markers in human tubal 
      epithelium verifies the importance of mesenchymal-epithelial interactions in 
      development of the human female reproductive tract. In a separate experiment, 
      DES-induction of uterine epithelial progesterone receptor (PGR) and estrogen 
      receptor 1 (ESR1) was explored in tissue recombinants composed of wild-type or 
      Esr1KO mouse uterine mesenchyme + human fetal uterine epithelium (wt UtM+hUtE and 
      Esr1KO UtM+hUtE). The rationale of this experiment was to determine whether 
      DES-induction of PGR and ESR1 is mediated directly via epithelial ESR1 or 
      indirectly (paracrine mechanism) via mesenchymal ESR1. DES-induction of uterine 
      epithelial ESR1 and PGR in Esr1KO UtM+hUtE tissue recombinants (devoid of 
      mesenchymal ESR1) formally eliminates the paracrine mechanism and demonstrates 
      that DES induction of human uterine epithelial ESR1 and PGR is directly mediated 
      via epithelial ESR1.
CI  - Copyright (c) 2018 International Society of Differentiation. Published by Elsevier 
      B.V. All rights reserved.
FAU - Cunha, Gerald R
AU  - Cunha GR
AD  - Department of Urology, University of California, 400 Parnassus Avenue, San 
      Francisco, CA 94143, United States. Electronic address: Gerald.Cunha@ucsf.edu.
FAU - Kurita, Takeshi
AU  - Kurita T
AD  - Department of Cancer Biology and Genetics, College of Medicine, Comprehensive 
      Cancer Center, Ohio State University, 812 Biomedical Research Tower, 460 W. 12th 
      Avenue, Columbus, OH 43210, United States.
FAU - Cao, Mei
AU  - Cao M
AD  - Department of Urology, University of California, 400 Parnassus Avenue, San 
      Francisco, CA 94143, United States.
FAU - Shen, Joel
AU  - Shen J
AD  - Department of Urology, University of California, 400 Parnassus Avenue, San 
      Francisco, CA 94143, United States.
FAU - Cooke, Paul S
AU  - Cooke PS
AD  - Department of Physiological Sciences, College of Veterinary Medicine, University 
      of Florida, 1333 Center Drive, Gainesville, FL 32610, United States.
FAU - Robboy, Stanley J
AU  - Robboy SJ
AD  - Department of Pathology, Duke University Medical Center, DUMC 3712, Durham, NC 
      27710, United States.
FAU - Baskin, Laurence S
AU  - Baskin LS
AD  - Department of Urology, University of California, 400 Parnassus Avenue, San 
      Francisco, CA 94143, United States.
LA  - eng
GR  - R01 CA154358/CA/NCI NIH HHS/United States
GR  - R01 DK058105/DK/NIDDK NIH HHS/United States
GR  - R21 HD088006/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20180418
PL  - England
TA  - Differentiation
JT  - Differentiation; research in biological diversity
JID - 0401650
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Receptors, Progesterone)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Epithelial Cells/cytology
MH  - Epithelium/*growth & development
MH  - Estrogen Receptor alpha/metabolism
MH  - Female
MH  - Genitalia, Female
MH  - Humans
MH  - Mesoderm/cytology
MH  - Mice
MH  - Receptors, Progesterone/*metabolism
MH  - Uterus/*growth & development/metabolism
PMC - PMC5993605
MID - NIHMS961365
OTO - NOTNLM
OT  - Diethylstilbestrol
OT  - Estrogen receptor
OT  - Estrogenic response
OT  - Human female fetal reproductive tract
OT  - Mesenchymal-epithelial interactions
OT  - Progesterone receptor
EDAT- 2018/04/24 06:00
MHDA- 2018/11/22 06:00
PMCR- 2019/05/01
CRDT- 2018/04/24 06:00
PHST- 2018/03/15 00:00 [received]
PHST- 2018/04/11 00:00 [revised]
PHST- 2018/04/16 00:00 [accepted]
PHST- 2018/04/24 06:00 [pubmed]
PHST- 2018/11/22 06:00 [medline]
PHST- 2018/04/24 06:00 [entrez]
PHST- 2019/05/01 00:00 [pmc-release]
AID - S0301-4681(18)30034-3 [pii]
AID - 10.1016/j.diff.2018.04.002 [doi]
PST - ppublish
SO  - Differentiation. 2018 May-Jun;101:39-45. doi: 10.1016/j.diff.2018.04.002. Epub 
      2018 Apr 18.