PMID- 29686531
OWN - NLM
STAT- MEDLINE
DCOM- 20180917
LR  - 20181114
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2018
DP  - 2018
TI  - Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition 
      of ASC Phosphorylation.
PG  - 6054069
LID - 10.1155/2018/6054069 [doi]
LID - 6054069
AB  - Artemisia princeps var. orientalis (Asteraceae, A. princeps) is a well-known 
      traditional medicinal herb used for treating various inflammatory disorders in 
      Korea, Japan, China, and other Asian countries. In the present study, we 
      investigated the effects of A. princeps extract (APO) on interleukin- (IL-) 1beta 
      regulation and inflammasome activation in bone marrow-derived macrophages (BMDMs) 
      and monosodium urate- (MSU-) induced peritonitis mouse model in vivo. The APO 
      treatment to BMDMs primed with lipopolysaccharide (LPS) attenuated the NLRP3 and 
      AIM2 inflammasome activation induced by danger signals, such as ATP, nigericin, 
      silica crystals, and poly (dA:dT), respectively. Mechanistic study revealed that 
      APO suppressed the ASC oligomerization and speck formation, which are required 
      for inflammasome activation. APO treatment also reduced the ASC phosphorylation 
      induced by the combination of LPS and a tyrosine phosphatase inhibitor. In vivo 
      evaluation revealed that intraperitoneal administration of APO reduced IL-1beta 
      levels, significantly (p < 0.05) and dose dependently, in the MSU-induced 
      peritonitis mouse model. In conclusion, our study is the first to report that the 
      extract of A. princeps inhibits inflammasome activation through the modulation of 
      ASC phosphorylation. Therefore, APO might be developed as therapeutic potential 
      in the treatment of inflammasome-mediated inflammatory disorders, such as gouty 
      arthritis.
FAU - Kwak, Su-Bin
AU  - Kwak SB
AD  - Department of Applied Life Science, Graduate School, Konkuk University, Chungju, 
      Republic of Korea.
FAU - Koppula, Sushruta
AU  - Koppula S
AD  - Department of Applied Life Science, Graduate School, Konkuk University, Chungju, 
      Republic of Korea.
FAU - In, Eun-Jung
AU  - In EJ
AD  - Department of Applied Life Science, Graduate School, Konkuk University, Chungju, 
      Republic of Korea.
FAU - Sun, Xiao
AU  - Sun X
AD  - Department of Applied Life Science, Graduate School, Konkuk University, Chungju, 
      Republic of Korea.
FAU - Kim, Young-Kyu
AU  - Kim YK
AD  - Department of Applied Life Science, Graduate School, Konkuk University, Chungju, 
      Republic of Korea.
FAU - Kim, Myong-Ki
AU  - Kim MK
AUID- ORCID: 0000-0002-2956-7006
AD  - Department of Food Science and Engineering, Seowon University, Cheongju, Republic 
      of Korea.
FAU - Lee, Kwang-Ho
AU  - Lee KH
AUID- ORCID: 0000-0002-3677-0977
AD  - Department of Applied Life Science, Graduate School, Konkuk University, Chungju, 
      Republic of Korea.
AD  - Department of Biotechnology, College of Biomedical & Health Science, Research 
      Institute of Inflammatory Diseases, 268 Chungwon-daero, Chungju, Republic of 
      Korea.
FAU - Kang, Tae-Bong
AU  - Kang TB
AUID- ORCID: 0000-0003-1441-9470
AD  - Department of Applied Life Science, Graduate School, Konkuk University, Chungju, 
      Republic of Korea.
AD  - Department of Biotechnology, College of Biomedical & Health Science, Research 
      Institute of Inflammatory Diseases, 268 Chungwon-daero, Chungju, Republic of 
      Korea.
LA  - eng
PT  - Journal Article
DEP - 20180304
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Aim2 protein, mouse)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Artemisia/*chemistry
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Antibody Technique
MH  - Inflammasomes/*drug effects/*metabolism
MH  - Interleukin-1beta/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/genetics/*metabolism
MH  - Phosphorylation/drug effects
MH  - Plant Extracts/chemistry/*therapeutic use
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC5857320
EDAT- 2018/04/25 06:00
MHDA- 2018/09/18 06:00
PMCR- 2018/03/04
CRDT- 2018/04/25 06:00
PHST- 2017/06/30 00:00 [received]
PHST- 2017/12/14 00:00 [revised]
PHST- 2018/01/03 00:00 [accepted]
PHST- 2018/04/25 06:00 [entrez]
PHST- 2018/04/25 06:00 [pubmed]
PHST- 2018/09/18 06:00 [medline]
PHST- 2018/03/04 00:00 [pmc-release]
AID - 10.1155/2018/6054069 [doi]
PST - epublish
SO  - Mediators Inflamm. 2018 Mar 4;2018:6054069. doi: 10.1155/2018/6054069. 
      eCollection 2018.