PMID- 29687170
OWN - NLM
STAT- MEDLINE
DCOM- 20181226
LR  - 20181226
IS  - 1573-5168 (Electronic)
IS  - 0920-1742 (Linking)
VI  - 44
IP  - 4
DP  - 2018 Aug
TI  - Identification, characterization, and expression analysis of adiponectin 
      receptors in black carp Mylopharyngodon piceus in response to dietary 
      carbohydrate.
PG  - 1127-1141
LID - 10.1007/s10695-018-0501-5 [doi]
AB  - In the present study, three cDNAs of AdipoRs (MpAdipoR1a, MpAdipoR1b, and 
      MpAdipoR2) were identified from juvenile black carp Mylopharyngodon piceus. There 
      were 375, 378, and 356 amino acids in the MpAdipoR1a, MpAdipoR1b, and MpAdipoR2, 
      respectively. BLAST analysis reveals that MpAdipoRs share high identities with 
      other known AdipoRs from zebrafish, rainbow trout, human, etc. And there were all 
      seven transmembrane regions in the amino acid sequences of MpAdipoR1s and 
      MpAdipoR2, respectively. The relative expression levels of MpAdipoR1s were higher 
      in the liver, blood, brain, and eyes in black carp (p < 0.05). Relatively higher 
      expression of MpAdipoR2 was detected in the liver (p < 0.05) and then in the 
      adipose tissues and blood by real-time PCR assays. The relative expression levels 
      of AdipoR mRNA in the liver, muscle, brain, blood, and adipose tissues were 
      detected by real-time PCR in black carp fed with four levels of dietary 
      carbohydrate (CHO) (10.65, 19.43, 28.84, and 37.91%) for 9 weeks, respectively. 
      The expression levels of MpAdipoR1s in the liver, muscle, brain, and blood were 
      induced and reached to the maximum at optimal dietary CHO (19.43 or 28.84%) and 
      then were decreased at 37.91% dietary CHO, although there were no significant 
      differences on the expression levels of MpAdipoR1a in the liver between 19.43, 
      28.84, and 37.91% dietary CHO. Similarly, MpAdipoR2 were also induced to the 
      maximal levels in the liver, muscle, brain, and blood at optimal dietary CHO 
      (19.43 or 28.84%) compared with that at 10.65% dietary CHO, although no 
      significant differences were observed on the expression levels of MpAdipoR2 in 
      the liver and muscle between groups fed 19.43 and 37.91% dietary CHO (p > 0.05). 
      However, the expression levels of MpAdipoRs in the adipose tissues were 
      significantly downregulated at excessive dietary CHO (37.91%) compared with other 
      groups (p < 0.05). These results indicated that MpAdipoRs were inducible proteins 
      and might enhance the understanding of their vital roles in the regulation of 
      glucose metabolic homeostasis in black carp.
FAU - Wu, Chenglong
AU  - Wu C
AD  - College of Life Science, Huzhou University, 759 East 2nd Road, Huzhou, 313000, 
      People's Republic of China. wuchenglong007@163.com.
FAU - Gao, Jun'e
AU  - Gao J
AD  - College of Life Science, Huzhou University, 759 East 2nd Road, Huzhou, 313000, 
      People's Republic of China.
FAU - Chen, Lian
AU  - Chen L
AD  - College of Life Science, Huzhou University, 759 East 2nd Road, Huzhou, 313000, 
      People's Republic of China.
FAU - Shao, Xianping
AU  - Shao X
AD  - College of Life Science, Huzhou University, 759 East 2nd Road, Huzhou, 313000, 
      People's Republic of China.
FAU - Ye, Jinyun
AU  - Ye J
AD  - College of Life Science, Huzhou University, 759 East 2nd Road, Huzhou, 313000, 
      People's Republic of China.
LA  - eng
GR  - 31672669, 31202008/National Natural Science Foundation of China/
GR  - LY12C19002/Zhejiang Provincial Natural Science Foundation of China/
GR  - pd2013410/the Young Academic Leaders in Colleges and Universities in Zhejiang 
      Province/
GR  - CARS-46/the Earmarked Fund for China Agriculture Research System/
PT  - Journal Article
DEP - 20180423
PL  - Netherlands
TA  - Fish Physiol Biochem
JT  - Fish physiology and biochemistry
JID - 100955049
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Fish Proteins)
RN  - 0 (Receptors, Adiponectin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carps/genetics/growth & development/*metabolism
MH  - Cloning, Molecular
MH  - Dietary Carbohydrates/*administration & dosage
MH  - Fish Proteins/genetics/*metabolism
MH  - *Gene Expression Regulation
MH  - Organ Specificity
MH  - Phylogeny
MH  - Receptors, Adiponectin/genetics/*metabolism
MH  - Sequence Homology
OTO - NOTNLM
OT  - Adiponectin receptor
OT  - Dietary carbohydrate
OT  - Mylopharyngodon piceus
OT  - cDNA cloning
OT  - mRNA expression
EDAT- 2018/04/25 06:00
MHDA- 2018/12/27 06:00
CRDT- 2018/04/25 06:00
PHST- 2017/03/12 00:00 [received]
PHST- 2018/04/11 00:00 [accepted]
PHST- 2018/04/25 06:00 [pubmed]
PHST- 2018/12/27 06:00 [medline]
PHST- 2018/04/25 06:00 [entrez]
AID - 10.1007/s10695-018-0501-5 [pii]
AID - 10.1007/s10695-018-0501-5 [doi]
PST - ppublish
SO  - Fish Physiol Biochem. 2018 Aug;44(4):1127-1141. doi: 10.1007/s10695-018-0501-5. 
      Epub 2018 Apr 23.